Diagnostic significance of immunoglobulin G avidity in symptomatic and asymptomatic West Nile virus infection
Abstract INTRODUCTION West Nile virus (WNV) immunoglobulin M (IgM) antibodies have been shown to persist for up to 500 days in certain patients. To evaluate the usefulness of immunoglobulin G (IgG) avidity assessment in the diagnosis of WNV infection, we analyzed 54 WNV IgM- and/or IgG-positive seru...
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ftdoajarticles:oai:doaj.org/article:fcc05a0df37d478babfe867b678463f6 2023-05-15T15:12:18+02:00 Diagnostic significance of immunoglobulin G avidity in symptomatic and asymptomatic West Nile virus infection Tatjana Vilibic-Cavlek Branimir Kristofic Vladimir Savic Branko Kolaric Ljubo Barbic Irena Tabain Ljiljana Peric Dario Sabadi Bozana Miklausic Tanja Potocnik-Hunjadi Sanja Zember Vladimir Stevanovic Eddy Listes Giovanni Savini https://doi.org/10.1590/0037-8682-0482-2017 https://doaj.org/article/fcc05a0df37d478babfe867b678463f6 EN eng Sociedade Brasileira de Medicina Tropical (SBMT) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822018000500591&lng=en&tlng=en https://doaj.org/toc/1678-9849 1678-9849 doi:10.1590/0037-8682-0482-2017 https://doaj.org/article/fcc05a0df37d478babfe867b678463f6 Revista da Sociedade Brasileira de Medicina Tropical, Vol 51, Iss 5, Pp 591-595 West Nile virus Diagnosis IgG avidity Arctic medicine. Tropical medicine RC955-962 article ftdoajarticles https://doi.org/10.1590/0037-8682-0482-2017 2022-12-31T02:57:32Z Abstract INTRODUCTION West Nile virus (WNV) immunoglobulin M (IgM) antibodies have been shown to persist for up to 500 days in certain patients. To evaluate the usefulness of immunoglobulin G (IgG) avidity assessment in the diagnosis of WNV infection, we analyzed 54 WNV IgM- and/or IgG-positive serum samples from 39 patients with neuroinvasive disease and 15 asymptomatic cases tested during a seroprevalence investigation. METHODS Serological tests (WNV IgM/IgG antibody detection, IgG avidity) were performed using commercially available enzyme-linked immunosorbent assays. RESULTS WNV IgM antibodies were detected in 47 (87%) samples. Acute/recent WNV infection was confirmed based on low/borderline avidity index (AI) in 44 IgM-positive samples (93.6%). In three IgM-positive samples (6.4%), high IgG AIs were detected, thus indicating persisting IgM antibodies from previous infections. All IgM-negative samples showed high AIs. Patients with WNV neuroinvasive disease tested within 30 days showed low AIs. In six patients tested 34-50 days after disease onset, AI was borderline (42%-60%), suggesting earlier WNV IgG maturation. Samples with the highest IgM values were associated with the lowest AIs (Spearman's rho coefficient -0.767, p < 0.001). CONCLUSIONS Our results indicate that IgG avidity differentiates current/recent WNV infection from persistent IgM seropositivity from the previous WNV transmission season both in patients with WNV neuroinvasive disease and in asymptomatic persons. A strong negative correlation between IgM antibody levels and AI indicates that in cases with very high IgM levels, determination of IgG avidity may not be necessary. As many patients showed rapid avidity maturation, low IgG avidity is indicative of WNV infection within the previous month. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Rho ENVELOPE(-63.000,-63.000,-64.300,-64.300) Revista da Sociedade Brasileira de Medicina Tropical 51 5 591 595 |
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Directory of Open Access Journals: DOAJ Articles |
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English |
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West Nile virus Diagnosis IgG avidity Arctic medicine. Tropical medicine RC955-962 |
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West Nile virus Diagnosis IgG avidity Arctic medicine. Tropical medicine RC955-962 Tatjana Vilibic-Cavlek Branimir Kristofic Vladimir Savic Branko Kolaric Ljubo Barbic Irena Tabain Ljiljana Peric Dario Sabadi Bozana Miklausic Tanja Potocnik-Hunjadi Sanja Zember Vladimir Stevanovic Eddy Listes Giovanni Savini Diagnostic significance of immunoglobulin G avidity in symptomatic and asymptomatic West Nile virus infection |
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West Nile virus Diagnosis IgG avidity Arctic medicine. Tropical medicine RC955-962 |
description |
Abstract INTRODUCTION West Nile virus (WNV) immunoglobulin M (IgM) antibodies have been shown to persist for up to 500 days in certain patients. To evaluate the usefulness of immunoglobulin G (IgG) avidity assessment in the diagnosis of WNV infection, we analyzed 54 WNV IgM- and/or IgG-positive serum samples from 39 patients with neuroinvasive disease and 15 asymptomatic cases tested during a seroprevalence investigation. METHODS Serological tests (WNV IgM/IgG antibody detection, IgG avidity) were performed using commercially available enzyme-linked immunosorbent assays. RESULTS WNV IgM antibodies were detected in 47 (87%) samples. Acute/recent WNV infection was confirmed based on low/borderline avidity index (AI) in 44 IgM-positive samples (93.6%). In three IgM-positive samples (6.4%), high IgG AIs were detected, thus indicating persisting IgM antibodies from previous infections. All IgM-negative samples showed high AIs. Patients with WNV neuroinvasive disease tested within 30 days showed low AIs. In six patients tested 34-50 days after disease onset, AI was borderline (42%-60%), suggesting earlier WNV IgG maturation. Samples with the highest IgM values were associated with the lowest AIs (Spearman's rho coefficient -0.767, p < 0.001). CONCLUSIONS Our results indicate that IgG avidity differentiates current/recent WNV infection from persistent IgM seropositivity from the previous WNV transmission season both in patients with WNV neuroinvasive disease and in asymptomatic persons. A strong negative correlation between IgM antibody levels and AI indicates that in cases with very high IgM levels, determination of IgG avidity may not be necessary. As many patients showed rapid avidity maturation, low IgG avidity is indicative of WNV infection within the previous month. |
format |
Article in Journal/Newspaper |
author |
Tatjana Vilibic-Cavlek Branimir Kristofic Vladimir Savic Branko Kolaric Ljubo Barbic Irena Tabain Ljiljana Peric Dario Sabadi Bozana Miklausic Tanja Potocnik-Hunjadi Sanja Zember Vladimir Stevanovic Eddy Listes Giovanni Savini |
author_facet |
Tatjana Vilibic-Cavlek Branimir Kristofic Vladimir Savic Branko Kolaric Ljubo Barbic Irena Tabain Ljiljana Peric Dario Sabadi Bozana Miklausic Tanja Potocnik-Hunjadi Sanja Zember Vladimir Stevanovic Eddy Listes Giovanni Savini |
author_sort |
Tatjana Vilibic-Cavlek |
title |
Diagnostic significance of immunoglobulin G avidity in symptomatic and asymptomatic West Nile virus infection |
title_short |
Diagnostic significance of immunoglobulin G avidity in symptomatic and asymptomatic West Nile virus infection |
title_full |
Diagnostic significance of immunoglobulin G avidity in symptomatic and asymptomatic West Nile virus infection |
title_fullStr |
Diagnostic significance of immunoglobulin G avidity in symptomatic and asymptomatic West Nile virus infection |
title_full_unstemmed |
Diagnostic significance of immunoglobulin G avidity in symptomatic and asymptomatic West Nile virus infection |
title_sort |
diagnostic significance of immunoglobulin g avidity in symptomatic and asymptomatic west nile virus infection |
publisher |
Sociedade Brasileira de Medicina Tropical (SBMT) |
url |
https://doi.org/10.1590/0037-8682-0482-2017 https://doaj.org/article/fcc05a0df37d478babfe867b678463f6 |
long_lat |
ENVELOPE(-63.000,-63.000,-64.300,-64.300) |
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Arctic Rho |
geographic_facet |
Arctic Rho |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista da Sociedade Brasileira de Medicina Tropical, Vol 51, Iss 5, Pp 591-595 |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822018000500591&lng=en&tlng=en https://doaj.org/toc/1678-9849 1678-9849 doi:10.1590/0037-8682-0482-2017 https://doaj.org/article/fcc05a0df37d478babfe867b678463f6 |
op_doi |
https://doi.org/10.1590/0037-8682-0482-2017 |
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Revista da Sociedade Brasileira de Medicina Tropical |
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51 |
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591 |
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