New Arctic Bacterial Isolates with Relevant Enzymatic Potential

• Published in: Molecules
• Main Authors: Michał Piegza, Wojciech Łaba, Miroslava Kačániová
• Format: Article in Journal/Newspaper
• Language: English
• Published: MDPI AG 2020
• Subjects: MALDI-TOF MS Biotyper; molecular; fluorescence; hydrolytic enzymes; Organic chemistry; QD241-441; Arctic; Spitzbergen
• Online Access: https://doi.org/10.3390/molecules25173930; https://doaj.org/article/fca9c34fef9545bfba80ceda6e49c8f1

Fragments of wood drifting in the vicinity of Spitzbergen were used for the isolation of microorganisms, carried out using atypical carbon sources: colloidal chitin, cellulose and carboxymethylcellulose, xylan, casein, tributrin and olive oil. Purified cultures were subjected to a three-step identification: with classical methods, using MALDI-TOF MS Biotyper whole-cell protein fingerprinting, and molecular analysis of 16S rDNA. Subsequently, a preliminary assessment of the enzymatic potential of isolates was carried out. As a result, cellulolytic activity was observed in more than 50% of the bacterial strains, exhibiting activity of 0.30–0.40 U/mL. Over 53% of the isolates demonstrated xylanolytic activity, of which the highest reached from 0.40 to 0.90 U. Polygalacturonase activity of 0.003–1.6 was also demonstrated in half of the bacterial strains studied. Proteolytic activity of isolates did not exceed 0.3 U. An important highlight was the ability of fluorescent dye production by certain strains, grown on skim milk-agar, but also on pure meat extract.