The response of Thalassiosira pseudonana to long-term exposure to increased CO2 and decreased pH.
The effect of ocean acidification conditions has been investigated in cultures of the diatom Thalassiosira pseudonana CCMP1335. Expected end-of-the-century pCO(2) (aq) concentrations of 760 µatm (equivalent to pH 7.8) were compared with present-day condition (380 µatm CO(2), pH 8.1). Batch culture p...
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ftdoajarticles:oai:doaj.org/article:fc9892889ed040379b259ccf8cab2d47 2023-05-15T17:51:35+02:00 The response of Thalassiosira pseudonana to long-term exposure to increased CO2 and decreased pH. Katharine J Crawfurd John A Raven Glen L Wheeler Emily J Baxter Ian Joint 2011-01-01T00:00:00Z https://doi.org/10.1371/journal.pone.0026695 https://doaj.org/article/fc9892889ed040379b259ccf8cab2d47 EN eng Public Library of Science (PLoS) https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22053201/pdf/?tool=EBI https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0026695 https://doaj.org/article/fc9892889ed040379b259ccf8cab2d47 PLoS ONE, Vol 6, Iss 10, p e26695 (2011) Medicine R Science Q article 2011 ftdoajarticles https://doi.org/10.1371/journal.pone.0026695 2022-12-31T05:28:27Z The effect of ocean acidification conditions has been investigated in cultures of the diatom Thalassiosira pseudonana CCMP1335. Expected end-of-the-century pCO(2) (aq) concentrations of 760 µatm (equivalent to pH 7.8) were compared with present-day condition (380 µatm CO(2), pH 8.1). Batch culture pH changed rapidly because of CO(2) (aq) assimilation and pH targets of 7.8 and 8.1 could not be sustained. Long-term (∼100 generation) pH-auxostat, continuous cultures could be maintained at target pH when cell density was kept low (<2×10(5) cells mL(-1)). After 3 months continuous culture, the C:N ratio was slightly decreased under high CO(2) conditions and red fluorescence per cell was slightly increased. However, no change was detected in photosynthetic efficiency (F(v)/F(m)) or functional cross section of PS II (σ(PSII)). Elevated pCO(2) has been predicted to be beneficial to diatoms due to reduced cost of carbon concentration mechanisms. There was reduced transcription of one putative δ-carbonic anhydrase (CA-4) after 3 months growth at increased CO(2) but 3 other δ-CAs and the small subunit of RUBISCO showed no change. There was no evidence of adaptation or clade selection of T. pseudonana after ∼100 generations at elevated CO(2). On the basis of this long-term culture, pH change of this magnitude in the future ocean may have little effect on T. pseudonana in the absence of genetic adaption. Article in Journal/Newspaper Ocean acidification Directory of Open Access Journals: DOAJ Articles PLoS ONE 6 10 e26695 |
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topic |
Medicine R Science Q |
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Medicine R Science Q Katharine J Crawfurd John A Raven Glen L Wheeler Emily J Baxter Ian Joint The response of Thalassiosira pseudonana to long-term exposure to increased CO2 and decreased pH. |
topic_facet |
Medicine R Science Q |
description |
The effect of ocean acidification conditions has been investigated in cultures of the diatom Thalassiosira pseudonana CCMP1335. Expected end-of-the-century pCO(2) (aq) concentrations of 760 µatm (equivalent to pH 7.8) were compared with present-day condition (380 µatm CO(2), pH 8.1). Batch culture pH changed rapidly because of CO(2) (aq) assimilation and pH targets of 7.8 and 8.1 could not be sustained. Long-term (∼100 generation) pH-auxostat, continuous cultures could be maintained at target pH when cell density was kept low (<2×10(5) cells mL(-1)). After 3 months continuous culture, the C:N ratio was slightly decreased under high CO(2) conditions and red fluorescence per cell was slightly increased. However, no change was detected in photosynthetic efficiency (F(v)/F(m)) or functional cross section of PS II (σ(PSII)). Elevated pCO(2) has been predicted to be beneficial to diatoms due to reduced cost of carbon concentration mechanisms. There was reduced transcription of one putative δ-carbonic anhydrase (CA-4) after 3 months growth at increased CO(2) but 3 other δ-CAs and the small subunit of RUBISCO showed no change. There was no evidence of adaptation or clade selection of T. pseudonana after ∼100 generations at elevated CO(2). On the basis of this long-term culture, pH change of this magnitude in the future ocean may have little effect on T. pseudonana in the absence of genetic adaption. |
format |
Article in Journal/Newspaper |
author |
Katharine J Crawfurd John A Raven Glen L Wheeler Emily J Baxter Ian Joint |
author_facet |
Katharine J Crawfurd John A Raven Glen L Wheeler Emily J Baxter Ian Joint |
author_sort |
Katharine J Crawfurd |
title |
The response of Thalassiosira pseudonana to long-term exposure to increased CO2 and decreased pH. |
title_short |
The response of Thalassiosira pseudonana to long-term exposure to increased CO2 and decreased pH. |
title_full |
The response of Thalassiosira pseudonana to long-term exposure to increased CO2 and decreased pH. |
title_fullStr |
The response of Thalassiosira pseudonana to long-term exposure to increased CO2 and decreased pH. |
title_full_unstemmed |
The response of Thalassiosira pseudonana to long-term exposure to increased CO2 and decreased pH. |
title_sort |
response of thalassiosira pseudonana to long-term exposure to increased co2 and decreased ph. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2011 |
url |
https://doi.org/10.1371/journal.pone.0026695 https://doaj.org/article/fc9892889ed040379b259ccf8cab2d47 |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_source |
PLoS ONE, Vol 6, Iss 10, p e26695 (2011) |
op_relation |
https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22053201/pdf/?tool=EBI https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0026695 https://doaj.org/article/fc9892889ed040379b259ccf8cab2d47 |
op_doi |
https://doi.org/10.1371/journal.pone.0026695 |
container_title |
PLoS ONE |
container_volume |
6 |
container_issue |
10 |
container_start_page |
e26695 |
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