Sequence Variability of pXO1-Located Pathogenicity Genes of Bacillus anthracis Natural Strains of Different Geographic Origin
The main pathogenic factor of Bacillus anthracis is a three-component toxin encoded by the pagA , lef , and cya genes, which are located on the pXO1 plasmid. The atxA gene, which encodes the primary regulator of pathogenicity factor expression, is located on the same plasmid. In this work, we evalua...
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2021
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ftdoajarticles:oai:doaj.org/article:fc1aa2cb4bb54895b1537668fcbde10b 2023-05-15T14:57:40+02:00 Sequence Variability of pXO1-Located Pathogenicity Genes of Bacillus anthracis Natural Strains of Different Geographic Origin Yulia Goncharova Irina Bahtejeva Galina Titareva Tatiana Kravchenko Anastasia Lev Ivan Dyatlov Vitalii Timofeev 2021-11-01T00:00:00Z https://doi.org/10.3390/pathogens10121556 https://doaj.org/article/fc1aa2cb4bb54895b1537668fcbde10b EN eng MDPI AG https://www.mdpi.com/2076-0817/10/12/1556 https://doaj.org/toc/2076-0817 doi:10.3390/pathogens10121556 2076-0817 https://doaj.org/article/fc1aa2cb4bb54895b1537668fcbde10b Pathogens, Vol 10, Iss 1556, p 1556 (2021) Bacillus anthracis pathogenicity factors genotyping Medicine R article 2021 ftdoajarticles https://doi.org/10.3390/pathogens10121556 2022-12-30T19:55:24Z The main pathogenic factor of Bacillus anthracis is a three-component toxin encoded by the pagA , lef , and cya genes, which are located on the pXO1 plasmid. The atxA gene, which encodes the primary regulator of pathogenicity factor expression, is located on the same plasmid. In this work, we evaluated the polymorphism of the pagA , lef , cya , and atxA genes for 85 B. anthracis strains from different evolutionary lineages and canSNP groups. We have found a strong correlation of 19 genotypes with the main evolutionary lineages, but the correlation with the canSNP group of the strain was not as strong. We have detected several genetic markers indicating the geographical origin of the strains, for example, their source from the steppe zone of the former USSR. We also found that strains of the B.Br.001/002 group caused an anthrax epidemic in Russia in 2016 and strains isolated during paleontological excavations in the Russian Arctic have the same genotype as the strains of the B.Br.CNEVA group circulating in Central Europe. This data could testify in favor of the genetic relationship of these two groups of strains and hypothesize the ways of distribution of their ancestral forms between Europe and the Arctic. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Paga ENVELOPE(70.833,70.833,73.267,73.267) Pathogens 10 12 1556 |
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Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Bacillus anthracis pathogenicity factors genotyping Medicine R |
spellingShingle |
Bacillus anthracis pathogenicity factors genotyping Medicine R Yulia Goncharova Irina Bahtejeva Galina Titareva Tatiana Kravchenko Anastasia Lev Ivan Dyatlov Vitalii Timofeev Sequence Variability of pXO1-Located Pathogenicity Genes of Bacillus anthracis Natural Strains of Different Geographic Origin |
topic_facet |
Bacillus anthracis pathogenicity factors genotyping Medicine R |
description |
The main pathogenic factor of Bacillus anthracis is a three-component toxin encoded by the pagA , lef , and cya genes, which are located on the pXO1 plasmid. The atxA gene, which encodes the primary regulator of pathogenicity factor expression, is located on the same plasmid. In this work, we evaluated the polymorphism of the pagA , lef , cya , and atxA genes for 85 B. anthracis strains from different evolutionary lineages and canSNP groups. We have found a strong correlation of 19 genotypes with the main evolutionary lineages, but the correlation with the canSNP group of the strain was not as strong. We have detected several genetic markers indicating the geographical origin of the strains, for example, their source from the steppe zone of the former USSR. We also found that strains of the B.Br.001/002 group caused an anthrax epidemic in Russia in 2016 and strains isolated during paleontological excavations in the Russian Arctic have the same genotype as the strains of the B.Br.CNEVA group circulating in Central Europe. This data could testify in favor of the genetic relationship of these two groups of strains and hypothesize the ways of distribution of their ancestral forms between Europe and the Arctic. |
format |
Article in Journal/Newspaper |
author |
Yulia Goncharova Irina Bahtejeva Galina Titareva Tatiana Kravchenko Anastasia Lev Ivan Dyatlov Vitalii Timofeev |
author_facet |
Yulia Goncharova Irina Bahtejeva Galina Titareva Tatiana Kravchenko Anastasia Lev Ivan Dyatlov Vitalii Timofeev |
author_sort |
Yulia Goncharova |
title |
Sequence Variability of pXO1-Located Pathogenicity Genes of Bacillus anthracis Natural Strains of Different Geographic Origin |
title_short |
Sequence Variability of pXO1-Located Pathogenicity Genes of Bacillus anthracis Natural Strains of Different Geographic Origin |
title_full |
Sequence Variability of pXO1-Located Pathogenicity Genes of Bacillus anthracis Natural Strains of Different Geographic Origin |
title_fullStr |
Sequence Variability of pXO1-Located Pathogenicity Genes of Bacillus anthracis Natural Strains of Different Geographic Origin |
title_full_unstemmed |
Sequence Variability of pXO1-Located Pathogenicity Genes of Bacillus anthracis Natural Strains of Different Geographic Origin |
title_sort |
sequence variability of pxo1-located pathogenicity genes of bacillus anthracis natural strains of different geographic origin |
publisher |
MDPI AG |
publishDate |
2021 |
url |
https://doi.org/10.3390/pathogens10121556 https://doaj.org/article/fc1aa2cb4bb54895b1537668fcbde10b |
long_lat |
ENVELOPE(70.833,70.833,73.267,73.267) |
geographic |
Arctic Paga |
geographic_facet |
Arctic Paga |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Pathogens, Vol 10, Iss 1556, p 1556 (2021) |
op_relation |
https://www.mdpi.com/2076-0817/10/12/1556 https://doaj.org/toc/2076-0817 doi:10.3390/pathogens10121556 2076-0817 https://doaj.org/article/fc1aa2cb4bb54895b1537668fcbde10b |
op_doi |
https://doi.org/10.3390/pathogens10121556 |
container_title |
Pathogens |
container_volume |
10 |
container_issue |
12 |
container_start_page |
1556 |
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1766329802106601472 |