Microsatellite based molecular epidemiology of Leishmania infantum from re-emerging foci of visceral leishmaniasis in Armenia and pilot risk assessment by ecological niche modeling.
Background Visceral leishmaniasis (VL) is re-emerging in Armenia since 1999 with 167 cases recorded until 2019. The objectives of this study were (i) to determine for the first time the genetic diversity and population structure of the causative agent of VL in Armenia; (ii) to compare these genotype...
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ftdoajarticles:oai:doaj.org/article:fbd1ee821db942c4b0cf0852c70867d4 2023-05-15T15:16:43+02:00 Microsatellite based molecular epidemiology of Leishmania infantum from re-emerging foci of visceral leishmaniasis in Armenia and pilot risk assessment by ecological niche modeling. Katrin Kuhls Olga Moskalenko Anna Sukiasyan Dezdemonia Manukyan Gayane Melik-Andreasyan Liana Atshemyan Hripsime Apresyan Margarita Strelkova Anja Jaeschke Ralf Wieland Marcus Frohme Sofia Cortes Ara Keshishyan 2021-04-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0009288 https://doaj.org/article/fbd1ee821db942c4b0cf0852c70867d4 EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0009288 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0009288 https://doaj.org/article/fbd1ee821db942c4b0cf0852c70867d4 PLoS Neglected Tropical Diseases, Vol 15, Iss 4, p e0009288 (2021) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2021 ftdoajarticles https://doi.org/10.1371/journal.pntd.0009288 2022-12-31T05:03:46Z Background Visceral leishmaniasis (VL) is re-emerging in Armenia since 1999 with 167 cases recorded until 2019. The objectives of this study were (i) to determine for the first time the genetic diversity and population structure of the causative agent of VL in Armenia; (ii) to compare these genotypes with those from most endemic regions worldwide; (iii) to monitor the diversity of vectors in Armenia; (iv) to predict the distribution of the vectors and VL in time and space by ecological niche modeling. Methodology/principal findings Human samples from different parts of Armenia previously identified by ITS-1-RFLP as L. infantum were studied by Multilocus Microsatellite Typing (MLMT). These data were combined with previously typed L. infantum strains from the main global endemic regions for population structure analysis. Within the 23 Armenian L. infantum strains 22 different genotypes were identified. The combined analysis revealed that all strains belong to the worldwide predominating MON1-population, however most closely related to a subpopulation from Southeastern Europe, Maghreb, Middle East and Central Asia. The three observed Armenian clusters grouped within this subpopulation with strains from Greece/Turkey, and from Central Asia, respectively. Ecological niche modeling based on VL cases and collected proven vectors (P. balcanicus, P. kandelakii) identified Yerevan and districts Lori, Tavush, Syunik, Armavir, Ararat bordering Georgia, Turkey, Iran and Azerbaijan as most suitable for the vectors and with the highest risk for VL transmission. Due to climate change the suitable habitat for VL transmission will expand in future all over Armenia. Conclusions Genetic diversity and population structure of the causative agent of VL in Armenia were addressed for the first time. Further genotyping studies should be performed with samples from infected humans, animals and sand flies from all active foci including the neighboring countries to understand transmission cycles, re-emergence, spread, and epidemiology of VL ... Article in Journal/Newspaper Arctic Climate change Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 15 4 e0009288 |
institution |
Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Katrin Kuhls Olga Moskalenko Anna Sukiasyan Dezdemonia Manukyan Gayane Melik-Andreasyan Liana Atshemyan Hripsime Apresyan Margarita Strelkova Anja Jaeschke Ralf Wieland Marcus Frohme Sofia Cortes Ara Keshishyan Microsatellite based molecular epidemiology of Leishmania infantum from re-emerging foci of visceral leishmaniasis in Armenia and pilot risk assessment by ecological niche modeling. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Background Visceral leishmaniasis (VL) is re-emerging in Armenia since 1999 with 167 cases recorded until 2019. The objectives of this study were (i) to determine for the first time the genetic diversity and population structure of the causative agent of VL in Armenia; (ii) to compare these genotypes with those from most endemic regions worldwide; (iii) to monitor the diversity of vectors in Armenia; (iv) to predict the distribution of the vectors and VL in time and space by ecological niche modeling. Methodology/principal findings Human samples from different parts of Armenia previously identified by ITS-1-RFLP as L. infantum were studied by Multilocus Microsatellite Typing (MLMT). These data were combined with previously typed L. infantum strains from the main global endemic regions for population structure analysis. Within the 23 Armenian L. infantum strains 22 different genotypes were identified. The combined analysis revealed that all strains belong to the worldwide predominating MON1-population, however most closely related to a subpopulation from Southeastern Europe, Maghreb, Middle East and Central Asia. The three observed Armenian clusters grouped within this subpopulation with strains from Greece/Turkey, and from Central Asia, respectively. Ecological niche modeling based on VL cases and collected proven vectors (P. balcanicus, P. kandelakii) identified Yerevan and districts Lori, Tavush, Syunik, Armavir, Ararat bordering Georgia, Turkey, Iran and Azerbaijan as most suitable for the vectors and with the highest risk for VL transmission. Due to climate change the suitable habitat for VL transmission will expand in future all over Armenia. Conclusions Genetic diversity and population structure of the causative agent of VL in Armenia were addressed for the first time. Further genotyping studies should be performed with samples from infected humans, animals and sand flies from all active foci including the neighboring countries to understand transmission cycles, re-emergence, spread, and epidemiology of VL ... |
format |
Article in Journal/Newspaper |
author |
Katrin Kuhls Olga Moskalenko Anna Sukiasyan Dezdemonia Manukyan Gayane Melik-Andreasyan Liana Atshemyan Hripsime Apresyan Margarita Strelkova Anja Jaeschke Ralf Wieland Marcus Frohme Sofia Cortes Ara Keshishyan |
author_facet |
Katrin Kuhls Olga Moskalenko Anna Sukiasyan Dezdemonia Manukyan Gayane Melik-Andreasyan Liana Atshemyan Hripsime Apresyan Margarita Strelkova Anja Jaeschke Ralf Wieland Marcus Frohme Sofia Cortes Ara Keshishyan |
author_sort |
Katrin Kuhls |
title |
Microsatellite based molecular epidemiology of Leishmania infantum from re-emerging foci of visceral leishmaniasis in Armenia and pilot risk assessment by ecological niche modeling. |
title_short |
Microsatellite based molecular epidemiology of Leishmania infantum from re-emerging foci of visceral leishmaniasis in Armenia and pilot risk assessment by ecological niche modeling. |
title_full |
Microsatellite based molecular epidemiology of Leishmania infantum from re-emerging foci of visceral leishmaniasis in Armenia and pilot risk assessment by ecological niche modeling. |
title_fullStr |
Microsatellite based molecular epidemiology of Leishmania infantum from re-emerging foci of visceral leishmaniasis in Armenia and pilot risk assessment by ecological niche modeling. |
title_full_unstemmed |
Microsatellite based molecular epidemiology of Leishmania infantum from re-emerging foci of visceral leishmaniasis in Armenia and pilot risk assessment by ecological niche modeling. |
title_sort |
microsatellite based molecular epidemiology of leishmania infantum from re-emerging foci of visceral leishmaniasis in armenia and pilot risk assessment by ecological niche modeling. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2021 |
url |
https://doi.org/10.1371/journal.pntd.0009288 https://doaj.org/article/fbd1ee821db942c4b0cf0852c70867d4 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic Climate change |
genre_facet |
Arctic Climate change |
op_source |
PLoS Neglected Tropical Diseases, Vol 15, Iss 4, p e0009288 (2021) |
op_relation |
https://doi.org/10.1371/journal.pntd.0009288 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0009288 https://doaj.org/article/fbd1ee821db942c4b0cf0852c70867d4 |
op_doi |
https://doi.org/10.1371/journal.pntd.0009288 |
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PLOS Neglected Tropical Diseases |
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15 |
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4 |
container_start_page |
e0009288 |
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