Reactive Oxygen Species Partly Mediate DNA Methylation in Responses to Different Heavy Metals in Pokeweed
DNA methylation is a rapid response strategy promoting plant survival under heavy metal (HM) stress. However, the roles of DNA methylation underlying plant adaptation to HM stress remain largely unknown. Here, we used pokeweed, a hyperaccumulator of manganese (Mn) and cadmium (Cd), to explore respon...
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ftdoajarticles:oai:doaj.org/article:fb9d78e9222e4268bed1e984c623e3f2 2023-05-15T16:01:43+02:00 Reactive Oxygen Species Partly Mediate DNA Methylation in Responses to Different Heavy Metals in Pokeweed Minyu Jing Hanchao Zhang Mingyue Wei Yongwei Tang Yan Xia Yahua Chen Zhenguo Shen Chen Chen 2022-04-01T00:00:00Z https://doi.org/10.3389/fpls.2022.845108 https://doaj.org/article/fb9d78e9222e4268bed1e984c623e3f2 EN eng Frontiers Media S.A. https://www.frontiersin.org/articles/10.3389/fpls.2022.845108/full https://doaj.org/toc/1664-462X 1664-462X doi:10.3389/fpls.2022.845108 https://doaj.org/article/fb9d78e9222e4268bed1e984c623e3f2 Frontiers in Plant Science, Vol 13 (2022) heavy metal pokeweed reactive oxygen species DNA methylation methylase and demethylase Plant culture SB1-1110 article 2022 ftdoajarticles https://doi.org/10.3389/fpls.2022.845108 2022-12-31T08:05:12Z DNA methylation is a rapid response strategy promoting plant survival under heavy metal (HM) stress. However, the roles of DNA methylation underlying plant adaptation to HM stress remain largely unknown. Here, we used pokeweed, a hyperaccumulator of manganese (Mn) and cadmium (Cd), to explore responses of plant to HM stress at phenotypic, transcriptional and DNA methylation levels. Mn- and Cd-specific response patterns were detected in pokeweed. The growth of pokeweed was both inhibited with exposure to excess Mn/Cd, but pokeweed distinguished Mn and Cd with different subcellular distributions, ROS scavenging systems, transcriptional patterns including genes involved in DNA methylation, and differentially methylated loci (DML). The number of DML between Mn/Cd treated and untreated samples increased with increased Mn/Cd concentrations. Meanwhile, pretreatment with NADPH oxidase inhibitors prior to HM exposure markedly reduced HM-induced reactive oxygen species (ROS), which caused reductions in expressions of DNA methylase and demethylase in pretreated samples. The increased levels of HM-induced demethylation were suppressed with alleviated ROS stress, and a series of HM-related methylated loci were also ROS-related. Taken together, our study demonstrates that different HMs affect different DNA methylation sites in a dose-dependent manner and changes in DNA methylation under Mn/Cd stress are partly mediated by HM-induced ROS. Article in Journal/Newspaper DML Directory of Open Access Journals: DOAJ Articles Frontiers in Plant Science 13 |
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Directory of Open Access Journals: DOAJ Articles |
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language |
English |
topic |
heavy metal pokeweed reactive oxygen species DNA methylation methylase and demethylase Plant culture SB1-1110 |
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heavy metal pokeweed reactive oxygen species DNA methylation methylase and demethylase Plant culture SB1-1110 Minyu Jing Hanchao Zhang Mingyue Wei Yongwei Tang Yan Xia Yahua Chen Zhenguo Shen Chen Chen Reactive Oxygen Species Partly Mediate DNA Methylation in Responses to Different Heavy Metals in Pokeweed |
topic_facet |
heavy metal pokeweed reactive oxygen species DNA methylation methylase and demethylase Plant culture SB1-1110 |
description |
DNA methylation is a rapid response strategy promoting plant survival under heavy metal (HM) stress. However, the roles of DNA methylation underlying plant adaptation to HM stress remain largely unknown. Here, we used pokeweed, a hyperaccumulator of manganese (Mn) and cadmium (Cd), to explore responses of plant to HM stress at phenotypic, transcriptional and DNA methylation levels. Mn- and Cd-specific response patterns were detected in pokeweed. The growth of pokeweed was both inhibited with exposure to excess Mn/Cd, but pokeweed distinguished Mn and Cd with different subcellular distributions, ROS scavenging systems, transcriptional patterns including genes involved in DNA methylation, and differentially methylated loci (DML). The number of DML between Mn/Cd treated and untreated samples increased with increased Mn/Cd concentrations. Meanwhile, pretreatment with NADPH oxidase inhibitors prior to HM exposure markedly reduced HM-induced reactive oxygen species (ROS), which caused reductions in expressions of DNA methylase and demethylase in pretreated samples. The increased levels of HM-induced demethylation were suppressed with alleviated ROS stress, and a series of HM-related methylated loci were also ROS-related. Taken together, our study demonstrates that different HMs affect different DNA methylation sites in a dose-dependent manner and changes in DNA methylation under Mn/Cd stress are partly mediated by HM-induced ROS. |
format |
Article in Journal/Newspaper |
author |
Minyu Jing Hanchao Zhang Mingyue Wei Yongwei Tang Yan Xia Yahua Chen Zhenguo Shen Chen Chen |
author_facet |
Minyu Jing Hanchao Zhang Mingyue Wei Yongwei Tang Yan Xia Yahua Chen Zhenguo Shen Chen Chen |
author_sort |
Minyu Jing |
title |
Reactive Oxygen Species Partly Mediate DNA Methylation in Responses to Different Heavy Metals in Pokeweed |
title_short |
Reactive Oxygen Species Partly Mediate DNA Methylation in Responses to Different Heavy Metals in Pokeweed |
title_full |
Reactive Oxygen Species Partly Mediate DNA Methylation in Responses to Different Heavy Metals in Pokeweed |
title_fullStr |
Reactive Oxygen Species Partly Mediate DNA Methylation in Responses to Different Heavy Metals in Pokeweed |
title_full_unstemmed |
Reactive Oxygen Species Partly Mediate DNA Methylation in Responses to Different Heavy Metals in Pokeweed |
title_sort |
reactive oxygen species partly mediate dna methylation in responses to different heavy metals in pokeweed |
publisher |
Frontiers Media S.A. |
publishDate |
2022 |
url |
https://doi.org/10.3389/fpls.2022.845108 https://doaj.org/article/fb9d78e9222e4268bed1e984c623e3f2 |
genre |
DML |
genre_facet |
DML |
op_source |
Frontiers in Plant Science, Vol 13 (2022) |
op_relation |
https://www.frontiersin.org/articles/10.3389/fpls.2022.845108/full https://doaj.org/toc/1664-462X 1664-462X doi:10.3389/fpls.2022.845108 https://doaj.org/article/fb9d78e9222e4268bed1e984c623e3f2 |
op_doi |
https://doi.org/10.3389/fpls.2022.845108 |
container_title |
Frontiers in Plant Science |
container_volume |
13 |
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1766397463758897152 |