Drug resistance of Mycobacterium tuberculosis strains in southern Brazil
INTRODUCTION: The aim of this work was to evaluate the prevalence of Mycobacterium tuberculosis (MT) strains with mutations that could result in resistance to the main drugs used in treatment in a region with one of the highest numbers of tuberculosis (TB) cases in southern Brazil. METHODS: Deoxyrib...
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ftdoajarticles:oai:doaj.org/article:fb8e37e0b4384e2c87c113a50a90342f 2023-05-15T15:10:15+02:00 Drug resistance of Mycobacterium tuberculosis strains in southern Brazil Laynara Katize Grutzmacher Eduardo Monguilhott Dalmarco Solange Lucia Blatt Caio Mauricio Mendes de Cordova 2012-02-01T00:00:00Z https://doi.org/10.1590/S0037-86822012000100018 https://doaj.org/article/fb8e37e0b4384e2c87c113a50a90342f EN eng Sociedade Brasileira de Medicina Tropical (SBMT) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822012000100018&lng=en&tlng=en https://doaj.org/toc/1678-9849 1678-9849 doi:10.1590/S0037-86822012000100018 https://doaj.org/article/fb8e37e0b4384e2c87c113a50a90342f Revista da Sociedade Brasileira de Medicina Tropical, Vol 45, Iss 1, Pp 95-99 (2012) Antibióticos Mycobacterium PCR-SSCP Resistência Tuberculose Arctic medicine. Tropical medicine RC955-962 article 2012 ftdoajarticles https://doi.org/10.1590/S0037-86822012000100018 2022-12-31T03:21:28Z INTRODUCTION: The aim of this work was to evaluate the prevalence of Mycobacterium tuberculosis (MT) strains with mutations that could result in resistance to the main drugs used in treatment in a region with one of the highest numbers of tuberculosis (TB) cases in southern Brazil. METHODS: Deoxyribonucleic acid (DNA) from 120 sputum samples from different patients suspicious of pulmonary tuberculosis who attended the Municipal Public Laboratory for Mycobacterium sp. diagnosis was directly amplified and analyzed by PCR-SSCP. The DNA was amplified in known hotspot mutation regions of the genes rpoB, ahpC, embB, katG, inhA, and pncA. RESULTS: The percentage of samples positive by culture was 9.2% (11/120); 5% (6/120) were positive by bacilloscopy and MT-PCR, and DNA fragments of the aforementioned resistance genes could be amplified from seven (7) of the eleven (11) samples with positive results, either by culture or PCR/bacilloscopy. All presented a SSCP pattern similar to a native, nonresistant genotype, with the ATCC strain 25177 as control, except for one sample (0.01%), which presented a SSCP profile demonstrating mutation at the embB gene. CONCLUSIONS: These results are consistent with the empirical observations by physicians treating TB patients in our region of a low occurrence of cases that are refractory to conventional treatment schemes, in contrast to other parts of the country. Continued surveillance, especially molecular, is essential to detect and monitor the outbreak of MT-resistant strains. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Revista da Sociedade Brasileira de Medicina Tropical 45 1 95 99 |
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Antibióticos Mycobacterium PCR-SSCP Resistência Tuberculose Arctic medicine. Tropical medicine RC955-962 |
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Antibióticos Mycobacterium PCR-SSCP Resistência Tuberculose Arctic medicine. Tropical medicine RC955-962 Laynara Katize Grutzmacher Eduardo Monguilhott Dalmarco Solange Lucia Blatt Caio Mauricio Mendes de Cordova Drug resistance of Mycobacterium tuberculosis strains in southern Brazil |
topic_facet |
Antibióticos Mycobacterium PCR-SSCP Resistência Tuberculose Arctic medicine. Tropical medicine RC955-962 |
description |
INTRODUCTION: The aim of this work was to evaluate the prevalence of Mycobacterium tuberculosis (MT) strains with mutations that could result in resistance to the main drugs used in treatment in a region with one of the highest numbers of tuberculosis (TB) cases in southern Brazil. METHODS: Deoxyribonucleic acid (DNA) from 120 sputum samples from different patients suspicious of pulmonary tuberculosis who attended the Municipal Public Laboratory for Mycobacterium sp. diagnosis was directly amplified and analyzed by PCR-SSCP. The DNA was amplified in known hotspot mutation regions of the genes rpoB, ahpC, embB, katG, inhA, and pncA. RESULTS: The percentage of samples positive by culture was 9.2% (11/120); 5% (6/120) were positive by bacilloscopy and MT-PCR, and DNA fragments of the aforementioned resistance genes could be amplified from seven (7) of the eleven (11) samples with positive results, either by culture or PCR/bacilloscopy. All presented a SSCP pattern similar to a native, nonresistant genotype, with the ATCC strain 25177 as control, except for one sample (0.01%), which presented a SSCP profile demonstrating mutation at the embB gene. CONCLUSIONS: These results are consistent with the empirical observations by physicians treating TB patients in our region of a low occurrence of cases that are refractory to conventional treatment schemes, in contrast to other parts of the country. Continued surveillance, especially molecular, is essential to detect and monitor the outbreak of MT-resistant strains. |
format |
Article in Journal/Newspaper |
author |
Laynara Katize Grutzmacher Eduardo Monguilhott Dalmarco Solange Lucia Blatt Caio Mauricio Mendes de Cordova |
author_facet |
Laynara Katize Grutzmacher Eduardo Monguilhott Dalmarco Solange Lucia Blatt Caio Mauricio Mendes de Cordova |
author_sort |
Laynara Katize Grutzmacher |
title |
Drug resistance of Mycobacterium tuberculosis strains in southern Brazil |
title_short |
Drug resistance of Mycobacterium tuberculosis strains in southern Brazil |
title_full |
Drug resistance of Mycobacterium tuberculosis strains in southern Brazil |
title_fullStr |
Drug resistance of Mycobacterium tuberculosis strains in southern Brazil |
title_full_unstemmed |
Drug resistance of Mycobacterium tuberculosis strains in southern Brazil |
title_sort |
drug resistance of mycobacterium tuberculosis strains in southern brazil |
publisher |
Sociedade Brasileira de Medicina Tropical (SBMT) |
publishDate |
2012 |
url |
https://doi.org/10.1590/S0037-86822012000100018 https://doaj.org/article/fb8e37e0b4384e2c87c113a50a90342f |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista da Sociedade Brasileira de Medicina Tropical, Vol 45, Iss 1, Pp 95-99 (2012) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822012000100018&lng=en&tlng=en https://doaj.org/toc/1678-9849 1678-9849 doi:10.1590/S0037-86822012000100018 https://doaj.org/article/fb8e37e0b4384e2c87c113a50a90342f |
op_doi |
https://doi.org/10.1590/S0037-86822012000100018 |
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Revista da Sociedade Brasileira de Medicina Tropical |
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45 |
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95 |
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99 |
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