A Proof-of-Concept Study to Develop a Peptide-Based Vaccine against Salmon Lice Infestation in Atlantic Salmon ( Salmo salar L.)
Proteins present in blood samples from Atlantic salmon ( Salmo salar ) infected with salmon lice ( Lepeophtheirus salmonis ) were analyzed using liquid chromatography–high-resolution mass spectrometry. Bioinformatic analyses revealed 1820 proteins, of which 58 were assigned to lice. Among these, per...
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MDPI AG
2024
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Online Access: | https://doi.org/10.3390/vaccines12050456 https://doaj.org/article/fb827666b0af4b7898b7e55a2b5b3a0a |
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ftdoajarticles:oai:doaj.org/article:fb827666b0af4b7898b7e55a2b5b3a0a 2024-09-15T17:56:14+00:00 A Proof-of-Concept Study to Develop a Peptide-Based Vaccine against Salmon Lice Infestation in Atlantic Salmon ( Salmo salar L.) Amritha Johny Pedro Ilardi Rolf Erik Olsen Bjørg Egelandsdal Erik Slinde 2024-04-01T00:00:00Z https://doi.org/10.3390/vaccines12050456 https://doaj.org/article/fb827666b0af4b7898b7e55a2b5b3a0a EN eng MDPI AG https://www.mdpi.com/2076-393X/12/5/456 https://doaj.org/toc/2076-393X doi:10.3390/vaccines12050456 2076-393X https://doaj.org/article/fb827666b0af4b7898b7e55a2b5b3a0a Vaccines, Vol 12, Iss 5, p 456 (2024) Lepeophtheirus salmonis Caligus rogercresseyi fish parasite blood feeding peptide epitope LC-MS/MS Medicine R article 2024 ftdoajarticles https://doi.org/10.3390/vaccines12050456 2024-08-05T17:49:20Z Proteins present in blood samples from Atlantic salmon ( Salmo salar ) infected with salmon lice ( Lepeophtheirus salmonis ) were analyzed using liquid chromatography–high-resolution mass spectrometry. Bioinformatic analyses revealed 1820 proteins, of which 58 were assigned to lice. Among these, peroxiredoxin-2, an antioxidant protein, was found relevant with respect to blood feeding of the parasite. The three-dimensional structure analysis of the protein revealed a surface amino acid sequence of interest. A 13-amino-acid peptide was selected as a potential antigen due to its predicted solubility, antigenicity, probable non-allergenic, and non-toxic nature. This peroxiredoxin-2-derived peptide was synthesized, combined with a commercially available adjuvant, and used for vaccination. The test vaccine demonstrated a 60–70% protection rate against early-stage Lepeophtheirus salmonis infection in a challenge trial in Norway. Additionally, the vaccine was tested against salmon lice ( Caligus rogercresseyi ) in Chile, where a remarkable 92% reduction in the number of adult lice was observed. Thus, in combination with the selected adjuvant, the peptide showed antigenic potential, making it a suitable candidate for future vaccine development. The approach described holds promise for the development of peptide vaccines against various ectoparasites feeding on blood or skin secretions of their hosts. Article in Journal/Newspaper Atlantic salmon Salmo salar Directory of Open Access Journals: DOAJ Articles Vaccines 12 5 456 |
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Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Lepeophtheirus salmonis Caligus rogercresseyi fish parasite blood feeding peptide epitope LC-MS/MS Medicine R |
spellingShingle |
Lepeophtheirus salmonis Caligus rogercresseyi fish parasite blood feeding peptide epitope LC-MS/MS Medicine R Amritha Johny Pedro Ilardi Rolf Erik Olsen Bjørg Egelandsdal Erik Slinde A Proof-of-Concept Study to Develop a Peptide-Based Vaccine against Salmon Lice Infestation in Atlantic Salmon ( Salmo salar L.) |
topic_facet |
Lepeophtheirus salmonis Caligus rogercresseyi fish parasite blood feeding peptide epitope LC-MS/MS Medicine R |
description |
Proteins present in blood samples from Atlantic salmon ( Salmo salar ) infected with salmon lice ( Lepeophtheirus salmonis ) were analyzed using liquid chromatography–high-resolution mass spectrometry. Bioinformatic analyses revealed 1820 proteins, of which 58 were assigned to lice. Among these, peroxiredoxin-2, an antioxidant protein, was found relevant with respect to blood feeding of the parasite. The three-dimensional structure analysis of the protein revealed a surface amino acid sequence of interest. A 13-amino-acid peptide was selected as a potential antigen due to its predicted solubility, antigenicity, probable non-allergenic, and non-toxic nature. This peroxiredoxin-2-derived peptide was synthesized, combined with a commercially available adjuvant, and used for vaccination. The test vaccine demonstrated a 60–70% protection rate against early-stage Lepeophtheirus salmonis infection in a challenge trial in Norway. Additionally, the vaccine was tested against salmon lice ( Caligus rogercresseyi ) in Chile, where a remarkable 92% reduction in the number of adult lice was observed. Thus, in combination with the selected adjuvant, the peptide showed antigenic potential, making it a suitable candidate for future vaccine development. The approach described holds promise for the development of peptide vaccines against various ectoparasites feeding on blood or skin secretions of their hosts. |
format |
Article in Journal/Newspaper |
author |
Amritha Johny Pedro Ilardi Rolf Erik Olsen Bjørg Egelandsdal Erik Slinde |
author_facet |
Amritha Johny Pedro Ilardi Rolf Erik Olsen Bjørg Egelandsdal Erik Slinde |
author_sort |
Amritha Johny |
title |
A Proof-of-Concept Study to Develop a Peptide-Based Vaccine against Salmon Lice Infestation in Atlantic Salmon ( Salmo salar L.) |
title_short |
A Proof-of-Concept Study to Develop a Peptide-Based Vaccine against Salmon Lice Infestation in Atlantic Salmon ( Salmo salar L.) |
title_full |
A Proof-of-Concept Study to Develop a Peptide-Based Vaccine against Salmon Lice Infestation in Atlantic Salmon ( Salmo salar L.) |
title_fullStr |
A Proof-of-Concept Study to Develop a Peptide-Based Vaccine against Salmon Lice Infestation in Atlantic Salmon ( Salmo salar L.) |
title_full_unstemmed |
A Proof-of-Concept Study to Develop a Peptide-Based Vaccine against Salmon Lice Infestation in Atlantic Salmon ( Salmo salar L.) |
title_sort |
proof-of-concept study to develop a peptide-based vaccine against salmon lice infestation in atlantic salmon ( salmo salar l.) |
publisher |
MDPI AG |
publishDate |
2024 |
url |
https://doi.org/10.3390/vaccines12050456 https://doaj.org/article/fb827666b0af4b7898b7e55a2b5b3a0a |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Vaccines, Vol 12, Iss 5, p 456 (2024) |
op_relation |
https://www.mdpi.com/2076-393X/12/5/456 https://doaj.org/toc/2076-393X doi:10.3390/vaccines12050456 2076-393X https://doaj.org/article/fb827666b0af4b7898b7e55a2b5b3a0a |
op_doi |
https://doi.org/10.3390/vaccines12050456 |
container_title |
Vaccines |
container_volume |
12 |
container_issue |
5 |
container_start_page |
456 |
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1810432445253681152 |