Association between EPHA5 methylation status in peripheral blood leukocytes and the risk and prognosis of gastric cancer
Purpose Altered DNA methylation, genetic alterations, and environmental factors are involved in tumorigenesis. As a tumor suppressor gene, abnormal EPHA5 methylation was found in gastric cancer (GC) tissues and was linked to the initiation, progression and prognosis of GC. In this study, the EPHA5 m...
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ftdoajarticles:oai:doaj.org/article:fb45d9d66b224f48b914eb59a5d61019 2024-01-07T09:45:49+01:00 Association between EPHA5 methylation status in peripheral blood leukocytes and the risk and prognosis of gastric cancer Xu Han Tianyu Liu Jiabao Zhai Chang Liu Wanyu Wang Chuang Nie Qi Wang Xiaojie Zhu Haibo Zhou Wenjing Tian 2022-09-01T00:00:00Z https://doi.org/10.7717/peerj.13774 https://doaj.org/article/fb45d9d66b224f48b914eb59a5d61019 EN eng PeerJ Inc. https://peerj.com/articles/13774.pdf https://peerj.com/articles/13774/ https://doaj.org/toc/2167-8359 doi:10.7717/peerj.13774 2167-8359 https://doaj.org/article/fb45d9d66b224f48b914eb59a5d61019 PeerJ, Vol 10, p e13774 (2022) Gastric cancer EPHA5 methylation Peripheral blood leukocytes Environmental factors Medicine R Biology (General) QH301-705.5 article 2022 ftdoajarticles https://doi.org/10.7717/peerj.13774 2023-12-10T01:53:53Z Purpose Altered DNA methylation, genetic alterations, and environmental factors are involved in tumorigenesis. As a tumor suppressor gene, abnormal EPHA5 methylation was found in gastric cancer (GC) tissues and was linked to the initiation, progression and prognosis of GC. In this study, the EPHA5 methylation level in peripheral blood leukocytes (PBLs) was detected to explore its relationship with GC risk and prognosis. Methods A total of 366 GC cases and 374 controls were selected as the subjects of this study to collect their environmental factors, and the EPHA5 methylation status was detected through the methylation-sensitive high-resolution melting method. Logistic regression analysis was utilized to evaluate the associations among EPHA5 methylation, environmental factors and GC risk. Meanwhile, the propensity score (PS) was used to adjust the imbalance of some independent variables. Results After PS adjustment, EPHA5 Pm (positive methylation) was more likely to increase the GC risk than EPHA5 Nm (negative methylation) (ORb = 1.827, 95% CI [1.202–2.777], P = 0.005). EPHA5 Pm had a more significant association with GC risk in the elderly (ORa = 2.785, 95% CI [1.563–4.961], P = 0.001) and H. pylori-negative groups (ORa = 2.758, 95% CI [1.369–5.555], P = 0.005). Moreover, the combined effects of EPHA5 Pm and H. pylori infection (ORca = 3.543, 95% CI [2.233–5.621], P < 0.001), consumption of alcohol (ORca = 2.893, 95% CI [1.844–4.539], P < 0.001), and salty food intake (ORca = 4.018, 95% CI [2.538–6.362], P < 0.001) on increasing the GC risk were observed. In addition, no convincing association was found between EPHA5 Pm and the GC prognosis. Conclusions EPHA5 methylation in PBLs and its combined effects with environmental risk factors are related to the GC risk. Article in Journal/Newspaper Orca Directory of Open Access Journals: DOAJ Articles Ora ENVELOPE(7.517,7.517,62.581,62.581) PeerJ 10 e13774 |
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English |
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Gastric cancer EPHA5 methylation Peripheral blood leukocytes Environmental factors Medicine R Biology (General) QH301-705.5 |
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Gastric cancer EPHA5 methylation Peripheral blood leukocytes Environmental factors Medicine R Biology (General) QH301-705.5 Xu Han Tianyu Liu Jiabao Zhai Chang Liu Wanyu Wang Chuang Nie Qi Wang Xiaojie Zhu Haibo Zhou Wenjing Tian Association between EPHA5 methylation status in peripheral blood leukocytes and the risk and prognosis of gastric cancer |
topic_facet |
Gastric cancer EPHA5 methylation Peripheral blood leukocytes Environmental factors Medicine R Biology (General) QH301-705.5 |
description |
Purpose Altered DNA methylation, genetic alterations, and environmental factors are involved in tumorigenesis. As a tumor suppressor gene, abnormal EPHA5 methylation was found in gastric cancer (GC) tissues and was linked to the initiation, progression and prognosis of GC. In this study, the EPHA5 methylation level in peripheral blood leukocytes (PBLs) was detected to explore its relationship with GC risk and prognosis. Methods A total of 366 GC cases and 374 controls were selected as the subjects of this study to collect their environmental factors, and the EPHA5 methylation status was detected through the methylation-sensitive high-resolution melting method. Logistic regression analysis was utilized to evaluate the associations among EPHA5 methylation, environmental factors and GC risk. Meanwhile, the propensity score (PS) was used to adjust the imbalance of some independent variables. Results After PS adjustment, EPHA5 Pm (positive methylation) was more likely to increase the GC risk than EPHA5 Nm (negative methylation) (ORb = 1.827, 95% CI [1.202–2.777], P = 0.005). EPHA5 Pm had a more significant association with GC risk in the elderly (ORa = 2.785, 95% CI [1.563–4.961], P = 0.001) and H. pylori-negative groups (ORa = 2.758, 95% CI [1.369–5.555], P = 0.005). Moreover, the combined effects of EPHA5 Pm and H. pylori infection (ORca = 3.543, 95% CI [2.233–5.621], P < 0.001), consumption of alcohol (ORca = 2.893, 95% CI [1.844–4.539], P < 0.001), and salty food intake (ORca = 4.018, 95% CI [2.538–6.362], P < 0.001) on increasing the GC risk were observed. In addition, no convincing association was found between EPHA5 Pm and the GC prognosis. Conclusions EPHA5 methylation in PBLs and its combined effects with environmental risk factors are related to the GC risk. |
format |
Article in Journal/Newspaper |
author |
Xu Han Tianyu Liu Jiabao Zhai Chang Liu Wanyu Wang Chuang Nie Qi Wang Xiaojie Zhu Haibo Zhou Wenjing Tian |
author_facet |
Xu Han Tianyu Liu Jiabao Zhai Chang Liu Wanyu Wang Chuang Nie Qi Wang Xiaojie Zhu Haibo Zhou Wenjing Tian |
author_sort |
Xu Han |
title |
Association between EPHA5 methylation status in peripheral blood leukocytes and the risk and prognosis of gastric cancer |
title_short |
Association between EPHA5 methylation status in peripheral blood leukocytes and the risk and prognosis of gastric cancer |
title_full |
Association between EPHA5 methylation status in peripheral blood leukocytes and the risk and prognosis of gastric cancer |
title_fullStr |
Association between EPHA5 methylation status in peripheral blood leukocytes and the risk and prognosis of gastric cancer |
title_full_unstemmed |
Association between EPHA5 methylation status in peripheral blood leukocytes and the risk and prognosis of gastric cancer |
title_sort |
association between epha5 methylation status in peripheral blood leukocytes and the risk and prognosis of gastric cancer |
publisher |
PeerJ Inc. |
publishDate |
2022 |
url |
https://doi.org/10.7717/peerj.13774 https://doaj.org/article/fb45d9d66b224f48b914eb59a5d61019 |
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ENVELOPE(7.517,7.517,62.581,62.581) |
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Ora |
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op_source |
PeerJ, Vol 10, p e13774 (2022) |
op_relation |
https://peerj.com/articles/13774.pdf https://peerj.com/articles/13774/ https://doaj.org/toc/2167-8359 doi:10.7717/peerj.13774 2167-8359 https://doaj.org/article/fb45d9d66b224f48b914eb59a5d61019 |
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https://doi.org/10.7717/peerj.13774 |
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