Bacteria associated with planktonic diatoms from Lake Baikal

• Published in: Acta Biologica Sibirica
• Main Authors: I. S. Mikhailov, Y. R. Zakharova, N. A. Volokitina, D. P. Petrova, Y. V. Likhoshway
• Format: Article in Journal/Newspaper
• Language: English
• Published: Altai State University 2018
• Subjects: diatoms; bacteria; 16S rRNA sequencing; Lake Baikal; Biology (General); QH301-705.5; Sibirica
• Online Access: https://doi.org/10.14258/abs.v4.i4.4880; https://doaj.org/article/fb3f2446cace4837bd038cc8a87e2ab1

Algal-bacterial associations were studied in unialgal xenic cultures of Synedra acus subsp. radians, Asterionella formosa and Fragillaria crotonensis planktonic diatoms from Lake Baikal, using epifluorescent and scanning electron microscopy. It was found that rod- and ovoid-shaped bacteria colonized cell walls of diatoms. Cloning and sequencing of fragments of 16S rRNA gene in diatom cultures revealed members of Gammaproteobacteria (Pseudomonas sp.), Betaproteobacteria (Janthinobacterium sp., Hydrogenophaga sp., Methylophilus sp.), Bacteroidetes (Flavobacterium sp., Pedobacter sp.), and Acinobacteria (Nocardioides sp.).