Improved methods for haemozoin quantification in tissues yield organ-and parasite-specific information in malaria-infected mice
Abstract Background Despite intensive research, malaria remains a major health concern for non-immune residents and travelers in malaria-endemic regions. Efficient adjunctive therapies against life-threatening complications such as severe malarial anaemia, encephalopathy, placental malaria or respir...
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ftdoajarticles:oai:doaj.org/article:fb3a1d7b9ecc43ccacaeb925cb6e725d 2023-05-15T15:16:03+02:00 Improved methods for haemozoin quantification in tissues yield organ-and parasite-specific information in malaria-infected mice Deroost Katrien Lays Natacha Noppen Sam Martens Erik Opdenakker Ghislain Van den Steen Philippe E 2012-05-01T00:00:00Z https://doi.org/10.1186/1475-2875-11-166 https://doaj.org/article/fb3a1d7b9ecc43ccacaeb925cb6e725d EN eng BMC http://www.malariajournal.com/content/11/1/166 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-11-166 1475-2875 https://doaj.org/article/fb3a1d7b9ecc43ccacaeb925cb6e725d Malaria Journal, Vol 11, Iss 1, p 166 (2012) Chemo-luminescence Densitometry Haemozoin quantification Malaria pigment Plasmodium Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2012 ftdoajarticles https://doi.org/10.1186/1475-2875-11-166 2022-12-31T08:10:20Z Abstract Background Despite intensive research, malaria remains a major health concern for non-immune residents and travelers in malaria-endemic regions. Efficient adjunctive therapies against life-threatening complications such as severe malarial anaemia, encephalopathy, placental malaria or respiratory problems are still lacking. Therefore, new insights into the pathogenesis of severe malaria are imperative. Haemozoin (Hz) or malaria pigment is produced during intra-erythrocytic parasite replication, released in the circulation after schizont rupture and accumulates inside multiple organs. Many in vitro and ex vivo immunomodulating effects are described for Hz but in vivo data are limited. This study aimed to improve methods for Hz quantification in tissues and to investigate the accumulation of Hz in different organs from mice infected with Plasmodium parasites with a varying degree of virulence. Methods An improved method for extraction of Hz from tissues was elaborated and coupled to an optimized, quantitative, microtiter plate-based luminescence assay with a high sensitivity. In addition, a technique for measuring Hz by semi-quantitative densitometry, applicable on transmitted light images, was developed. The methods were applied to measure Hz in various organs of C57BL/6 J mice infected with Plasmodium berghei ANKA, P. berghei NK65 or Plasmodium chabaudi AS. The used statistical methods were the Mann–Whitney U test and Pearsons correlation analysis. Results Most Hz was detected in livers and spleens, lower levels in lungs and kidneys, whereas sub-nanomolar amounts were observed in brains and hearts from infected mice, irrespectively of the parasite strain used. Furthermore, total Hz contents correlated with peripheral parasitaemia and were significantly higher in mice with a lethal P. berghei ANKA or P. berghei NK65-infection than in mice with a self-resolving P. chabaudi AS-infection, despite similar peripheral parasitaemia levels. Conclusions The developed techniques were useful to quantify Hz in ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 11 1 166 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Chemo-luminescence Densitometry Haemozoin quantification Malaria pigment Plasmodium Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Chemo-luminescence Densitometry Haemozoin quantification Malaria pigment Plasmodium Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Deroost Katrien Lays Natacha Noppen Sam Martens Erik Opdenakker Ghislain Van den Steen Philippe E Improved methods for haemozoin quantification in tissues yield organ-and parasite-specific information in malaria-infected mice |
topic_facet |
Chemo-luminescence Densitometry Haemozoin quantification Malaria pigment Plasmodium Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Despite intensive research, malaria remains a major health concern for non-immune residents and travelers in malaria-endemic regions. Efficient adjunctive therapies against life-threatening complications such as severe malarial anaemia, encephalopathy, placental malaria or respiratory problems are still lacking. Therefore, new insights into the pathogenesis of severe malaria are imperative. Haemozoin (Hz) or malaria pigment is produced during intra-erythrocytic parasite replication, released in the circulation after schizont rupture and accumulates inside multiple organs. Many in vitro and ex vivo immunomodulating effects are described for Hz but in vivo data are limited. This study aimed to improve methods for Hz quantification in tissues and to investigate the accumulation of Hz in different organs from mice infected with Plasmodium parasites with a varying degree of virulence. Methods An improved method for extraction of Hz from tissues was elaborated and coupled to an optimized, quantitative, microtiter plate-based luminescence assay with a high sensitivity. In addition, a technique for measuring Hz by semi-quantitative densitometry, applicable on transmitted light images, was developed. The methods were applied to measure Hz in various organs of C57BL/6 J mice infected with Plasmodium berghei ANKA, P. berghei NK65 or Plasmodium chabaudi AS. The used statistical methods were the Mann–Whitney U test and Pearsons correlation analysis. Results Most Hz was detected in livers and spleens, lower levels in lungs and kidneys, whereas sub-nanomolar amounts were observed in brains and hearts from infected mice, irrespectively of the parasite strain used. Furthermore, total Hz contents correlated with peripheral parasitaemia and were significantly higher in mice with a lethal P. berghei ANKA or P. berghei NK65-infection than in mice with a self-resolving P. chabaudi AS-infection, despite similar peripheral parasitaemia levels. Conclusions The developed techniques were useful to quantify Hz in ... |
format |
Article in Journal/Newspaper |
author |
Deroost Katrien Lays Natacha Noppen Sam Martens Erik Opdenakker Ghislain Van den Steen Philippe E |
author_facet |
Deroost Katrien Lays Natacha Noppen Sam Martens Erik Opdenakker Ghislain Van den Steen Philippe E |
author_sort |
Deroost Katrien |
title |
Improved methods for haemozoin quantification in tissues yield organ-and parasite-specific information in malaria-infected mice |
title_short |
Improved methods for haemozoin quantification in tissues yield organ-and parasite-specific information in malaria-infected mice |
title_full |
Improved methods for haemozoin quantification in tissues yield organ-and parasite-specific information in malaria-infected mice |
title_fullStr |
Improved methods for haemozoin quantification in tissues yield organ-and parasite-specific information in malaria-infected mice |
title_full_unstemmed |
Improved methods for haemozoin quantification in tissues yield organ-and parasite-specific information in malaria-infected mice |
title_sort |
improved methods for haemozoin quantification in tissues yield organ-and parasite-specific information in malaria-infected mice |
publisher |
BMC |
publishDate |
2012 |
url |
https://doi.org/10.1186/1475-2875-11-166 https://doaj.org/article/fb3a1d7b9ecc43ccacaeb925cb6e725d |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 11, Iss 1, p 166 (2012) |
op_relation |
http://www.malariajournal.com/content/11/1/166 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-11-166 1475-2875 https://doaj.org/article/fb3a1d7b9ecc43ccacaeb925cb6e725d |
op_doi |
https://doi.org/10.1186/1475-2875-11-166 |
container_title |
Malaria Journal |
container_volume |
11 |
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166 |
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1766346365841965056 |