Testing the efficacy of a multi-component DNA-prime/DNA-boost vaccine against Trypanosoma cruzi infection in dogs.
Trypanosoma cruzi, the etiologic agent of Chagas Disease, is a major vector borne health problem in Latin America and an emerging infectious disease in the United States.We tested the efficacy of a multi-component DNA-prime/DNA-boost vaccine (TcVac1) against experimental T. cruzi infection in a cani...
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Online Access: | https://doi.org/10.1371/journal.pntd.0001050 https://doaj.org/article/fb0bfbc18f5e4f02b4366676719b137b |
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ftdoajarticles:oai:doaj.org/article:fb0bfbc18f5e4f02b4366676719b137b 2023-05-15T15:11:49+02:00 Testing the efficacy of a multi-component DNA-prime/DNA-boost vaccine against Trypanosoma cruzi infection in dogs. José E Aparicio-Burgos Laucel Ochoa-García José Antonio Zepeda-Escobar Shivali Gupta Monisha Dhiman José Simón Martínez Roberto Montes de Oca-Jiménez Margarita Val Arreola Alberto Barbabosa-Pliego Juan C Vázquez-Chagoyán Nisha Jain Garg 2011-01-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0001050 https://doaj.org/article/fb0bfbc18f5e4f02b4366676719b137b EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC3098890?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0001050 https://doaj.org/article/fb0bfbc18f5e4f02b4366676719b137b PLoS Neglected Tropical Diseases, Vol 5, Iss 5, p e1050 (2011) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2011 ftdoajarticles https://doi.org/10.1371/journal.pntd.0001050 2022-12-31T01:32:59Z Trypanosoma cruzi, the etiologic agent of Chagas Disease, is a major vector borne health problem in Latin America and an emerging infectious disease in the United States.We tested the efficacy of a multi-component DNA-prime/DNA-boost vaccine (TcVac1) against experimental T. cruzi infection in a canine model. Dogs were immunized with antigen-encoding plasmids and cytokine adjuvants, and two weeks after the last immunization, challenged with T. cruzi trypomastigotes. We measured antibody responses by ELISA and haemagglutination assay, parasitemia and infectivity to triatomines by xenodiagnosis, and performed electrocardiography and histology to assess myocardial damage and tissue pathology.Vaccination with TcVac1 elicited parasite-and antigen-specific IgM and IgG (IgG2>IgG1) responses. Upon challenge infection, TcVac1-vaccinated dogs, as compared to non-vaccinated controls dogs, responded to T. cruzi with a rapid expansion of antibody response, moderately enhanced CD8(+) T cell proliferation and IFN-γ production, and suppression of phagocytes' activity evidenced by decreased myeloperoxidase and nitrite levels. Subsequently, vaccinated dogs controlled the acute parasitemia by day 37 pi (44 dpi in non-vaccinated dogs), and exhibited a moderate decline in infectivity to triatomines. TcVac1-immunized dogs did not control the myocardial parasite burden and electrocardiographic and histopatholgic cardiac alterations that are the hallmarks of acute Chagas disease. During the chronic stage, TcVac1-vaccinated dogs exhibited a moderate decline in cardiac alterations determined by EKG and anatomo-/histo-pathological analysis while chronically-infected/non-vaccinated dogs continued to exhibit severe EKG alterations.Overall, these results demonstrated that TcVac1 provided a partial resistance to T. cruzi infection and Chagas disease, and provide an impetus to improve the vaccination strategy against Chagas disease. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLoS Neglected Tropical Diseases 5 5 e1050 |
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Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 José E Aparicio-Burgos Laucel Ochoa-García José Antonio Zepeda-Escobar Shivali Gupta Monisha Dhiman José Simón Martínez Roberto Montes de Oca-Jiménez Margarita Val Arreola Alberto Barbabosa-Pliego Juan C Vázquez-Chagoyán Nisha Jain Garg Testing the efficacy of a multi-component DNA-prime/DNA-boost vaccine against Trypanosoma cruzi infection in dogs. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Trypanosoma cruzi, the etiologic agent of Chagas Disease, is a major vector borne health problem in Latin America and an emerging infectious disease in the United States.We tested the efficacy of a multi-component DNA-prime/DNA-boost vaccine (TcVac1) against experimental T. cruzi infection in a canine model. Dogs were immunized with antigen-encoding plasmids and cytokine adjuvants, and two weeks after the last immunization, challenged with T. cruzi trypomastigotes. We measured antibody responses by ELISA and haemagglutination assay, parasitemia and infectivity to triatomines by xenodiagnosis, and performed electrocardiography and histology to assess myocardial damage and tissue pathology.Vaccination with TcVac1 elicited parasite-and antigen-specific IgM and IgG (IgG2>IgG1) responses. Upon challenge infection, TcVac1-vaccinated dogs, as compared to non-vaccinated controls dogs, responded to T. cruzi with a rapid expansion of antibody response, moderately enhanced CD8(+) T cell proliferation and IFN-γ production, and suppression of phagocytes' activity evidenced by decreased myeloperoxidase and nitrite levels. Subsequently, vaccinated dogs controlled the acute parasitemia by day 37 pi (44 dpi in non-vaccinated dogs), and exhibited a moderate decline in infectivity to triatomines. TcVac1-immunized dogs did not control the myocardial parasite burden and electrocardiographic and histopatholgic cardiac alterations that are the hallmarks of acute Chagas disease. During the chronic stage, TcVac1-vaccinated dogs exhibited a moderate decline in cardiac alterations determined by EKG and anatomo-/histo-pathological analysis while chronically-infected/non-vaccinated dogs continued to exhibit severe EKG alterations.Overall, these results demonstrated that TcVac1 provided a partial resistance to T. cruzi infection and Chagas disease, and provide an impetus to improve the vaccination strategy against Chagas disease. |
format |
Article in Journal/Newspaper |
author |
José E Aparicio-Burgos Laucel Ochoa-García José Antonio Zepeda-Escobar Shivali Gupta Monisha Dhiman José Simón Martínez Roberto Montes de Oca-Jiménez Margarita Val Arreola Alberto Barbabosa-Pliego Juan C Vázquez-Chagoyán Nisha Jain Garg |
author_facet |
José E Aparicio-Burgos Laucel Ochoa-García José Antonio Zepeda-Escobar Shivali Gupta Monisha Dhiman José Simón Martínez Roberto Montes de Oca-Jiménez Margarita Val Arreola Alberto Barbabosa-Pliego Juan C Vázquez-Chagoyán Nisha Jain Garg |
author_sort |
José E Aparicio-Burgos |
title |
Testing the efficacy of a multi-component DNA-prime/DNA-boost vaccine against Trypanosoma cruzi infection in dogs. |
title_short |
Testing the efficacy of a multi-component DNA-prime/DNA-boost vaccine against Trypanosoma cruzi infection in dogs. |
title_full |
Testing the efficacy of a multi-component DNA-prime/DNA-boost vaccine against Trypanosoma cruzi infection in dogs. |
title_fullStr |
Testing the efficacy of a multi-component DNA-prime/DNA-boost vaccine against Trypanosoma cruzi infection in dogs. |
title_full_unstemmed |
Testing the efficacy of a multi-component DNA-prime/DNA-boost vaccine against Trypanosoma cruzi infection in dogs. |
title_sort |
testing the efficacy of a multi-component dna-prime/dna-boost vaccine against trypanosoma cruzi infection in dogs. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2011 |
url |
https://doi.org/10.1371/journal.pntd.0001050 https://doaj.org/article/fb0bfbc18f5e4f02b4366676719b137b |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 5, Iss 5, p e1050 (2011) |
op_relation |
http://europepmc.org/articles/PMC3098890?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0001050 https://doaj.org/article/fb0bfbc18f5e4f02b4366676719b137b |
op_doi |
https://doi.org/10.1371/journal.pntd.0001050 |
container_title |
PLoS Neglected Tropical Diseases |
container_volume |
5 |
container_issue |
5 |
container_start_page |
e1050 |
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1766342606050033664 |