Helminth species specific expansion and increased TNF-alpha production of non-classical monocytes during active tuberculosis.
Both Mycobacterium tuberculosis infection and helminths may affect innate immune mechanisms such as differential effects on monocytes towards the non-classical and intermediate subsets that favor bacterial persistence. Our aim, was to investigate helminth species specific effects on the frequency an...
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ftdoajarticles:oai:doaj.org/article:fad2f5adb4694c37ba8fd8f55938cb72 2023-05-15T15:14:31+02:00 Helminth species specific expansion and increased TNF-alpha production of non-classical monocytes during active tuberculosis. Gezahegn Bewket Amare Kiflie Ebba Abate Olle Stendahl Thomas Schön Robert Blomgran 2021-03-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0009194 https://doaj.org/article/fad2f5adb4694c37ba8fd8f55938cb72 EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0009194 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0009194 https://doaj.org/article/fad2f5adb4694c37ba8fd8f55938cb72 PLoS Neglected Tropical Diseases, Vol 15, Iss 3, p e0009194 (2021) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2021 ftdoajarticles https://doi.org/10.1371/journal.pntd.0009194 2022-12-31T15:20:13Z Both Mycobacterium tuberculosis infection and helminths may affect innate immune mechanisms such as differential effects on monocytes towards the non-classical and intermediate subsets that favor bacterial persistence. Our aim, was to investigate helminth species specific effects on the frequency and functional activity of monocyte subsets in patients with active tuberculosis and healthy subjects. HIV-negative patients with active pulmonary tuberculosis (PTB) and community controls (CCs) in Gondar, Ethiopia were screened for helminth infection by stool microscopy. Flow cytometric analysis of peripheral blood mononuclear cells (PBMCs) and ex vivo stimulation with purified protein derivative (PPD) and helminth antigens were used to characterize the distribution of monocyte subsets and their function. A total of 74 PTB patients and 57 CCs with and without helminth infection were included. Non-classical monocytes were increased in PTB patients with Ascaris and hookworm infection but not in Schistosoma-infected patients. Ascaris had the strongest effect in increasing the frequency of non-classical monocytes in both PTB patients and CCs, whereas PTB without helminth infection did not affect the frequency of monocyte subsets. There was a helminth specific increase in the frequency of TNF-α producing non-classical monocytes in hookworm infected PTB patients, both with and without PPD-stimulation. Low-to-intermediate TB disease severity associated with increased frequency of non-classical monocytes only for helminth-positive PTB patients, and the frequency of TNF-α producing monocytes were significantly higher in intermediate and non-classical monocytes of helminth positive PTB patients with an intermediate disease score. Helminth infection affected the frequency of monocyte subsets and function both in TB patients and controls which was helminth species dependent in TB patients. The clinical role of this potential immunomodulatory effect needs further study and may affect the response and protection to tuberculosis in ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 15 3 e0009194 |
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Directory of Open Access Journals: DOAJ Articles |
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English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Gezahegn Bewket Amare Kiflie Ebba Abate Olle Stendahl Thomas Schön Robert Blomgran Helminth species specific expansion and increased TNF-alpha production of non-classical monocytes during active tuberculosis. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Both Mycobacterium tuberculosis infection and helminths may affect innate immune mechanisms such as differential effects on monocytes towards the non-classical and intermediate subsets that favor bacterial persistence. Our aim, was to investigate helminth species specific effects on the frequency and functional activity of monocyte subsets in patients with active tuberculosis and healthy subjects. HIV-negative patients with active pulmonary tuberculosis (PTB) and community controls (CCs) in Gondar, Ethiopia were screened for helminth infection by stool microscopy. Flow cytometric analysis of peripheral blood mononuclear cells (PBMCs) and ex vivo stimulation with purified protein derivative (PPD) and helminth antigens were used to characterize the distribution of monocyte subsets and their function. A total of 74 PTB patients and 57 CCs with and without helminth infection were included. Non-classical monocytes were increased in PTB patients with Ascaris and hookworm infection but not in Schistosoma-infected patients. Ascaris had the strongest effect in increasing the frequency of non-classical monocytes in both PTB patients and CCs, whereas PTB without helminth infection did not affect the frequency of monocyte subsets. There was a helminth specific increase in the frequency of TNF-α producing non-classical monocytes in hookworm infected PTB patients, both with and without PPD-stimulation. Low-to-intermediate TB disease severity associated with increased frequency of non-classical monocytes only for helminth-positive PTB patients, and the frequency of TNF-α producing monocytes were significantly higher in intermediate and non-classical monocytes of helminth positive PTB patients with an intermediate disease score. Helminth infection affected the frequency of monocyte subsets and function both in TB patients and controls which was helminth species dependent in TB patients. The clinical role of this potential immunomodulatory effect needs further study and may affect the response and protection to tuberculosis in ... |
format |
Article in Journal/Newspaper |
author |
Gezahegn Bewket Amare Kiflie Ebba Abate Olle Stendahl Thomas Schön Robert Blomgran |
author_facet |
Gezahegn Bewket Amare Kiflie Ebba Abate Olle Stendahl Thomas Schön Robert Blomgran |
author_sort |
Gezahegn Bewket |
title |
Helminth species specific expansion and increased TNF-alpha production of non-classical monocytes during active tuberculosis. |
title_short |
Helminth species specific expansion and increased TNF-alpha production of non-classical monocytes during active tuberculosis. |
title_full |
Helminth species specific expansion and increased TNF-alpha production of non-classical monocytes during active tuberculosis. |
title_fullStr |
Helminth species specific expansion and increased TNF-alpha production of non-classical monocytes during active tuberculosis. |
title_full_unstemmed |
Helminth species specific expansion and increased TNF-alpha production of non-classical monocytes during active tuberculosis. |
title_sort |
helminth species specific expansion and increased tnf-alpha production of non-classical monocytes during active tuberculosis. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2021 |
url |
https://doi.org/10.1371/journal.pntd.0009194 https://doaj.org/article/fad2f5adb4694c37ba8fd8f55938cb72 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 15, Iss 3, p e0009194 (2021) |
op_relation |
https://doi.org/10.1371/journal.pntd.0009194 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0009194 https://doaj.org/article/fad2f5adb4694c37ba8fd8f55938cb72 |
op_doi |
https://doi.org/10.1371/journal.pntd.0009194 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
15 |
container_issue |
3 |
container_start_page |
e0009194 |
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1766344969923067904 |