Comprehensive characterization of plasma cell-free Echinococcus spp. DNA in echinococcosis patients using ultra-high-throughput sequencing.
BACKGROUND:Echinococcosis is a life-threatening parasitic disease caused by Echinococcus spp. tapeworms with over one million people affected globally at any time. The Echinococcus spp. tapeworms in the human body release DNA to the circulatory system, which can be a biomarker for echinococcosis. Ce...
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ftdoajarticles:oai:doaj.org/article:fad25e1dd70d46deac6ddc2e9f15ee07 2023-05-15T15:16:25+02:00 Comprehensive characterization of plasma cell-free Echinococcus spp. DNA in echinococcosis patients using ultra-high-throughput sequencing. Jingkai Ji Bin Li Jingzhong Li Wangmu Danzeng Jiandong Li Yanping Zhao Gezhen Qiangba Qingda Zhang Nibu Renzhen Zhuoga Basang Changlin Jia Quzhen Gongsang Jinmin Ma Yicong Wang Fang Chen Hongcheng Zhou Huasang Jiefang Yin Jiandan Xie Na Pei Huimin Cai Huayan Jiang Huanming Yang Jian Wang Asan Xiumin Han Junhua Li Weijun Chen Dong Yang 2020-04-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0008148 https://doaj.org/article/fad25e1dd70d46deac6ddc2e9f15ee07 EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0008148 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0008148 https://doaj.org/article/fad25e1dd70d46deac6ddc2e9f15ee07 PLoS Neglected Tropical Diseases, Vol 14, Iss 4, p e0008148 (2020) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2020 ftdoajarticles https://doi.org/10.1371/journal.pntd.0008148 2022-12-31T10:07:33Z BACKGROUND:Echinococcosis is a life-threatening parasitic disease caused by Echinococcus spp. tapeworms with over one million people affected globally at any time. The Echinococcus spp. tapeworms in the human body release DNA to the circulatory system, which can be a biomarker for echinococcosis. Cell-free DNA (cfDNA) is widely used in medical research and has been applied in various clinical settings. As for echinococcosis, several PCR-based tests had been trialed to detect cell-free Echinococcus spp. DNA in plasma or serum, but the sensitivity was about 20% to 25%. Low sensitivity of PCR-based methods might be related to our limited understanding of the features of cell-free Echinococcus spp. DNA in plasma, including its concentration, fragment pattern and release source. In this study, we applied ultra-high-throughput sequencing to comprehensively investigate the characteristics of cell-free Echinococcus spp. DNA in plasma of echinococcosis patients. METHODOLOGY/PRINCIPAL FINDINGS:We collected plasma samples from 23 echinococcosis patients. Total plasma cfDNA was extracted and sequenced with a high-throughput sequencing platform. An average of 282 million read pairs were obtained for each plasma sample. Sequencing data were analyzed with bioinformatics workflow combined with Echinococcus spp. sequence database. After identification of cell-free Echinococcus spp. reads, we found that the cell-free Echinococcus spp. reads accounted for 1.8e-5 to 4.0e-9 of the total clean reads. Comparing fragment length distribution of cfDNA between Echinococcus spp. and humans showed that cell-free Echinococcus spp. DNA of cystic echinococcosis (CE) had a broad length range, while that of alveolar echinococcosis (AE) had an obvious peak at about 135 bp. We found that most of the cell-free Echinococcus spp. DNA reads were from the nuclear genome with an even distribution, which might indicate a random release pattern of cell-free Echinococcus spp. DNA. CONCLUSIONS/SIGNIFICANCE:With ultra-high-throughput sequencing technology, ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 14 4 e0008148 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Jingkai Ji Bin Li Jingzhong Li Wangmu Danzeng Jiandong Li Yanping Zhao Gezhen Qiangba Qingda Zhang Nibu Renzhen Zhuoga Basang Changlin Jia Quzhen Gongsang Jinmin Ma Yicong Wang Fang Chen Hongcheng Zhou Huasang Jiefang Yin Jiandan Xie Na Pei Huimin Cai Huayan Jiang Huanming Yang Jian Wang Asan Xiumin Han Junhua Li Weijun Chen Dong Yang Comprehensive characterization of plasma cell-free Echinococcus spp. DNA in echinococcosis patients using ultra-high-throughput sequencing. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
BACKGROUND:Echinococcosis is a life-threatening parasitic disease caused by Echinococcus spp. tapeworms with over one million people affected globally at any time. The Echinococcus spp. tapeworms in the human body release DNA to the circulatory system, which can be a biomarker for echinococcosis. Cell-free DNA (cfDNA) is widely used in medical research and has been applied in various clinical settings. As for echinococcosis, several PCR-based tests had been trialed to detect cell-free Echinococcus spp. DNA in plasma or serum, but the sensitivity was about 20% to 25%. Low sensitivity of PCR-based methods might be related to our limited understanding of the features of cell-free Echinococcus spp. DNA in plasma, including its concentration, fragment pattern and release source. In this study, we applied ultra-high-throughput sequencing to comprehensively investigate the characteristics of cell-free Echinococcus spp. DNA in plasma of echinococcosis patients. METHODOLOGY/PRINCIPAL FINDINGS:We collected plasma samples from 23 echinococcosis patients. Total plasma cfDNA was extracted and sequenced with a high-throughput sequencing platform. An average of 282 million read pairs were obtained for each plasma sample. Sequencing data were analyzed with bioinformatics workflow combined with Echinococcus spp. sequence database. After identification of cell-free Echinococcus spp. reads, we found that the cell-free Echinococcus spp. reads accounted for 1.8e-5 to 4.0e-9 of the total clean reads. Comparing fragment length distribution of cfDNA between Echinococcus spp. and humans showed that cell-free Echinococcus spp. DNA of cystic echinococcosis (CE) had a broad length range, while that of alveolar echinococcosis (AE) had an obvious peak at about 135 bp. We found that most of the cell-free Echinococcus spp. DNA reads were from the nuclear genome with an even distribution, which might indicate a random release pattern of cell-free Echinococcus spp. DNA. CONCLUSIONS/SIGNIFICANCE:With ultra-high-throughput sequencing technology, ... |
format |
Article in Journal/Newspaper |
author |
Jingkai Ji Bin Li Jingzhong Li Wangmu Danzeng Jiandong Li Yanping Zhao Gezhen Qiangba Qingda Zhang Nibu Renzhen Zhuoga Basang Changlin Jia Quzhen Gongsang Jinmin Ma Yicong Wang Fang Chen Hongcheng Zhou Huasang Jiefang Yin Jiandan Xie Na Pei Huimin Cai Huayan Jiang Huanming Yang Jian Wang Asan Xiumin Han Junhua Li Weijun Chen Dong Yang |
author_facet |
Jingkai Ji Bin Li Jingzhong Li Wangmu Danzeng Jiandong Li Yanping Zhao Gezhen Qiangba Qingda Zhang Nibu Renzhen Zhuoga Basang Changlin Jia Quzhen Gongsang Jinmin Ma Yicong Wang Fang Chen Hongcheng Zhou Huasang Jiefang Yin Jiandan Xie Na Pei Huimin Cai Huayan Jiang Huanming Yang Jian Wang Asan Xiumin Han Junhua Li Weijun Chen Dong Yang |
author_sort |
Jingkai Ji |
title |
Comprehensive characterization of plasma cell-free Echinococcus spp. DNA in echinococcosis patients using ultra-high-throughput sequencing. |
title_short |
Comprehensive characterization of plasma cell-free Echinococcus spp. DNA in echinococcosis patients using ultra-high-throughput sequencing. |
title_full |
Comprehensive characterization of plasma cell-free Echinococcus spp. DNA in echinococcosis patients using ultra-high-throughput sequencing. |
title_fullStr |
Comprehensive characterization of plasma cell-free Echinococcus spp. DNA in echinococcosis patients using ultra-high-throughput sequencing. |
title_full_unstemmed |
Comprehensive characterization of plasma cell-free Echinococcus spp. DNA in echinococcosis patients using ultra-high-throughput sequencing. |
title_sort |
comprehensive characterization of plasma cell-free echinococcus spp. dna in echinococcosis patients using ultra-high-throughput sequencing. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2020 |
url |
https://doi.org/10.1371/journal.pntd.0008148 https://doaj.org/article/fad25e1dd70d46deac6ddc2e9f15ee07 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 14, Iss 4, p e0008148 (2020) |
op_relation |
https://doi.org/10.1371/journal.pntd.0008148 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0008148 https://doaj.org/article/fad25e1dd70d46deac6ddc2e9f15ee07 |
op_doi |
https://doi.org/10.1371/journal.pntd.0008148 |
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PLOS Neglected Tropical Diseases |
container_volume |
14 |
container_issue |
4 |
container_start_page |
e0008148 |
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1766346713955565568 |