Enhanced protective efficacy of nonpathogenic recombinant leishmania tarentolae expressing cysteine proteinases combined with a sand fly salivary antigen.
Novel vaccination approaches are needed to prevent leishmaniasis. Live attenuated vaccines are the gold standard for protection against intracellular pathogens such as Leishmania and there have been new developments in this field. The nonpathogenic to humans lizard protozoan parasite, Leishmania (L)...
Published in: | PLoS Neglected Tropical Diseases |
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2014
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ftdoajarticles:oai:doaj.org/article:faa0c8c3f68d4f0d9adf31ab49d10cb5 2023-05-15T15:10:49+02:00 Enhanced protective efficacy of nonpathogenic recombinant leishmania tarentolae expressing cysteine proteinases combined with a sand fly salivary antigen. Farnaz Zahedifard Elham Gholami Tahereh Taheri Yasaman Taslimi Fatemeh Doustdari Negar Seyed Fatemeh Torkashvand Claudio Meneses Barbara Papadopoulou Shaden Kamhawi Jesus G Valenzuela Sima Rafati 2014-03-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0002751 https://doaj.org/article/faa0c8c3f68d4f0d9adf31ab49d10cb5 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC3967951?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0002751 https://doaj.org/article/faa0c8c3f68d4f0d9adf31ab49d10cb5 PLoS Neglected Tropical Diseases, Vol 8, Iss 3, p e2751 (2014) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2014 ftdoajarticles https://doi.org/10.1371/journal.pntd.0002751 2022-12-31T15:18:19Z Novel vaccination approaches are needed to prevent leishmaniasis. Live attenuated vaccines are the gold standard for protection against intracellular pathogens such as Leishmania and there have been new developments in this field. The nonpathogenic to humans lizard protozoan parasite, Leishmania (L) tarentolae, has been used effectively as a vaccine platform against visceral leishmaniasis in experimental animal models. Correspondingly, pre-exposure to sand fly saliva or immunization with a salivary protein has been shown to protect mice against cutaneous leishmaniasis.Here, we tested the efficacy of a novel combination of established protective parasite antigens expressed by L. tarentolae together with a sand fly salivary antigen as a vaccine strategy against L. major infection. The immunogenicity and protective efficacy of different DNA/Live and Live/Live prime-boost vaccination modalities with live recombinant L. tarentolae stably expressing cysteine proteinases (type I and II, CPA/CPB) and PpSP15, an immunogenic salivary protein from Phlebotomus papatasi, a natural vector of L. major, were tested both in susceptible BALB/c and resistant C57BL/6 mice. Both humoral and cellular immune responses were assessed before challenge and at 3 and 10 weeks after Leishmania infection. In both strains of mice, the strongest protective effect was observed when priming with PpSP15 DNA and boosting with PpSP15 DNA and live recombinant L. tarentolae stably expressing cysteine proteinase genes.The present study is the first to use a combination of recombinant L. tarentolae with a sand fly salivary antigen (PpSP15) and represents a novel promising vaccination approach against leishmaniasis. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLoS Neglected Tropical Diseases 8 3 e2751 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
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English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Farnaz Zahedifard Elham Gholami Tahereh Taheri Yasaman Taslimi Fatemeh Doustdari Negar Seyed Fatemeh Torkashvand Claudio Meneses Barbara Papadopoulou Shaden Kamhawi Jesus G Valenzuela Sima Rafati Enhanced protective efficacy of nonpathogenic recombinant leishmania tarentolae expressing cysteine proteinases combined with a sand fly salivary antigen. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Novel vaccination approaches are needed to prevent leishmaniasis. Live attenuated vaccines are the gold standard for protection against intracellular pathogens such as Leishmania and there have been new developments in this field. The nonpathogenic to humans lizard protozoan parasite, Leishmania (L) tarentolae, has been used effectively as a vaccine platform against visceral leishmaniasis in experimental animal models. Correspondingly, pre-exposure to sand fly saliva or immunization with a salivary protein has been shown to protect mice against cutaneous leishmaniasis.Here, we tested the efficacy of a novel combination of established protective parasite antigens expressed by L. tarentolae together with a sand fly salivary antigen as a vaccine strategy against L. major infection. The immunogenicity and protective efficacy of different DNA/Live and Live/Live prime-boost vaccination modalities with live recombinant L. tarentolae stably expressing cysteine proteinases (type I and II, CPA/CPB) and PpSP15, an immunogenic salivary protein from Phlebotomus papatasi, a natural vector of L. major, were tested both in susceptible BALB/c and resistant C57BL/6 mice. Both humoral and cellular immune responses were assessed before challenge and at 3 and 10 weeks after Leishmania infection. In both strains of mice, the strongest protective effect was observed when priming with PpSP15 DNA and boosting with PpSP15 DNA and live recombinant L. tarentolae stably expressing cysteine proteinase genes.The present study is the first to use a combination of recombinant L. tarentolae with a sand fly salivary antigen (PpSP15) and represents a novel promising vaccination approach against leishmaniasis. |
format |
Article in Journal/Newspaper |
author |
Farnaz Zahedifard Elham Gholami Tahereh Taheri Yasaman Taslimi Fatemeh Doustdari Negar Seyed Fatemeh Torkashvand Claudio Meneses Barbara Papadopoulou Shaden Kamhawi Jesus G Valenzuela Sima Rafati |
author_facet |
Farnaz Zahedifard Elham Gholami Tahereh Taheri Yasaman Taslimi Fatemeh Doustdari Negar Seyed Fatemeh Torkashvand Claudio Meneses Barbara Papadopoulou Shaden Kamhawi Jesus G Valenzuela Sima Rafati |
author_sort |
Farnaz Zahedifard |
title |
Enhanced protective efficacy of nonpathogenic recombinant leishmania tarentolae expressing cysteine proteinases combined with a sand fly salivary antigen. |
title_short |
Enhanced protective efficacy of nonpathogenic recombinant leishmania tarentolae expressing cysteine proteinases combined with a sand fly salivary antigen. |
title_full |
Enhanced protective efficacy of nonpathogenic recombinant leishmania tarentolae expressing cysteine proteinases combined with a sand fly salivary antigen. |
title_fullStr |
Enhanced protective efficacy of nonpathogenic recombinant leishmania tarentolae expressing cysteine proteinases combined with a sand fly salivary antigen. |
title_full_unstemmed |
Enhanced protective efficacy of nonpathogenic recombinant leishmania tarentolae expressing cysteine proteinases combined with a sand fly salivary antigen. |
title_sort |
enhanced protective efficacy of nonpathogenic recombinant leishmania tarentolae expressing cysteine proteinases combined with a sand fly salivary antigen. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2014 |
url |
https://doi.org/10.1371/journal.pntd.0002751 https://doaj.org/article/faa0c8c3f68d4f0d9adf31ab49d10cb5 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 8, Iss 3, p e2751 (2014) |
op_relation |
http://europepmc.org/articles/PMC3967951?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0002751 https://doaj.org/article/faa0c8c3f68d4f0d9adf31ab49d10cb5 |
op_doi |
https://doi.org/10.1371/journal.pntd.0002751 |
container_title |
PLoS Neglected Tropical Diseases |
container_volume |
8 |
container_issue |
3 |
container_start_page |
e2751 |
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1766341761052966912 |