Case Studies in Polar Bear ( Ursus maritimus ) Sperm Collection and Cryopreservation Techniques
Assisted reproductive technologies can aid conservation efforts via support of ex situ population management and preservation of genetic material. Data from 38 sperm collection attempts from 17 polar bears (1–5 procedures/bear) were evaluated. Sample collections were attempted via electroejaculation...
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ftdoajarticles:oai:doaj.org/article:fa6fcb66391d4bf58a3bb0fb010be290 2023-05-15T18:42:26+02:00 Case Studies in Polar Bear ( Ursus maritimus ) Sperm Collection and Cryopreservation Techniques Jessye Wojtusik Terri L. Roth Erin Curry 2022-02-01T00:00:00Z https://doi.org/10.3390/ani12040430 https://doaj.org/article/fa6fcb66391d4bf58a3bb0fb010be290 EN eng MDPI AG https://www.mdpi.com/2076-2615/12/4/430 https://doaj.org/toc/2076-2615 doi:10.3390/ani12040430 2076-2615 https://doaj.org/article/fa6fcb66391d4bf58a3bb0fb010be290 Animals, Vol 12, Iss 430, p 430 (2022) electroejaculation endangered species gamete rescue polar bear sperm cryopreservation sperm rescue Veterinary medicine SF600-1100 Zoology QL1-991 article 2022 ftdoajarticles https://doi.org/10.3390/ani12040430 2022-12-31T16:03:31Z Assisted reproductive technologies can aid conservation efforts via support of ex situ population management and preservation of genetic material. Data from 38 sperm collection attempts from 17 polar bears (1–5 procedures/bear) were evaluated. Sample collections were attempted via electroejaculation (EEJ; n = 6), urethral catheterization (UC; n = 25), or sperm rescue (SR; n = 7) during the breeding season (Jan. 1-May 21; n = 27) and nonbreeding season (May 22-Dec. 31; n = 11). Sperm retrieval was successful in 1 EEJ (16.7%), 18 UC (72.0%) and 4 SR (57.1%) collections. Initial sperm motility and viability were 50.0% and 77.0% for EEJ, 64.3 ± 7.4% and 80.9 ± 3.8% for UC, and 56.7 ± 8.8% and 80.5 ± 0.5% for SR. UC and SR were more likely to be successful during the breeding season (84.2–100%) than the nonbreeding season (25.0–33.3%). Testicular tumors were observed in four males (57%) during SR. In total, 13 samples were cryopreserved ( n = 1 EEJ, 9 UC, and 3 SR) with egg-yolk-based equine extender (EQ) or OptiXcell (OP). For both extenders, post-thaw motility and viability were reduced by 20–60% and 30–65%, respectively. Further efforts to optimize procedures are warranted, but this summary provides data useful for enhancing the success of polar bear sperm collection and cryopreservation. Article in Journal/Newspaper Ursus maritimus Directory of Open Access Journals: DOAJ Articles Animals 12 4 430 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
electroejaculation endangered species gamete rescue polar bear sperm cryopreservation sperm rescue Veterinary medicine SF600-1100 Zoology QL1-991 |
spellingShingle |
electroejaculation endangered species gamete rescue polar bear sperm cryopreservation sperm rescue Veterinary medicine SF600-1100 Zoology QL1-991 Jessye Wojtusik Terri L. Roth Erin Curry Case Studies in Polar Bear ( Ursus maritimus ) Sperm Collection and Cryopreservation Techniques |
topic_facet |
electroejaculation endangered species gamete rescue polar bear sperm cryopreservation sperm rescue Veterinary medicine SF600-1100 Zoology QL1-991 |
description |
Assisted reproductive technologies can aid conservation efforts via support of ex situ population management and preservation of genetic material. Data from 38 sperm collection attempts from 17 polar bears (1–5 procedures/bear) were evaluated. Sample collections were attempted via electroejaculation (EEJ; n = 6), urethral catheterization (UC; n = 25), or sperm rescue (SR; n = 7) during the breeding season (Jan. 1-May 21; n = 27) and nonbreeding season (May 22-Dec. 31; n = 11). Sperm retrieval was successful in 1 EEJ (16.7%), 18 UC (72.0%) and 4 SR (57.1%) collections. Initial sperm motility and viability were 50.0% and 77.0% for EEJ, 64.3 ± 7.4% and 80.9 ± 3.8% for UC, and 56.7 ± 8.8% and 80.5 ± 0.5% for SR. UC and SR were more likely to be successful during the breeding season (84.2–100%) than the nonbreeding season (25.0–33.3%). Testicular tumors were observed in four males (57%) during SR. In total, 13 samples were cryopreserved ( n = 1 EEJ, 9 UC, and 3 SR) with egg-yolk-based equine extender (EQ) or OptiXcell (OP). For both extenders, post-thaw motility and viability were reduced by 20–60% and 30–65%, respectively. Further efforts to optimize procedures are warranted, but this summary provides data useful for enhancing the success of polar bear sperm collection and cryopreservation. |
format |
Article in Journal/Newspaper |
author |
Jessye Wojtusik Terri L. Roth Erin Curry |
author_facet |
Jessye Wojtusik Terri L. Roth Erin Curry |
author_sort |
Jessye Wojtusik |
title |
Case Studies in Polar Bear ( Ursus maritimus ) Sperm Collection and Cryopreservation Techniques |
title_short |
Case Studies in Polar Bear ( Ursus maritimus ) Sperm Collection and Cryopreservation Techniques |
title_full |
Case Studies in Polar Bear ( Ursus maritimus ) Sperm Collection and Cryopreservation Techniques |
title_fullStr |
Case Studies in Polar Bear ( Ursus maritimus ) Sperm Collection and Cryopreservation Techniques |
title_full_unstemmed |
Case Studies in Polar Bear ( Ursus maritimus ) Sperm Collection and Cryopreservation Techniques |
title_sort |
case studies in polar bear ( ursus maritimus ) sperm collection and cryopreservation techniques |
publisher |
MDPI AG |
publishDate |
2022 |
url |
https://doi.org/10.3390/ani12040430 https://doaj.org/article/fa6fcb66391d4bf58a3bb0fb010be290 |
genre |
Ursus maritimus |
genre_facet |
Ursus maritimus |
op_source |
Animals, Vol 12, Iss 430, p 430 (2022) |
op_relation |
https://www.mdpi.com/2076-2615/12/4/430 https://doaj.org/toc/2076-2615 doi:10.3390/ani12040430 2076-2615 https://doaj.org/article/fa6fcb66391d4bf58a3bb0fb010be290 |
op_doi |
https://doi.org/10.3390/ani12040430 |
container_title |
Animals |
container_volume |
12 |
container_issue |
4 |
container_start_page |
430 |
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1766232086826450944 |