ESTUDO DA IMOBILIZAÇÃO DE LIPASE EM SÍLICA OBTIDA PELA TÉCNICA SOL-GEL
The objective of this work was the immobilization of the enzyme Candida antarctica lipase B (CAL B) using the sol-gel method of immobilization and three different initiators of the polymerization reaction: one acid (HCl), one basic (NH4OH) and the other nucleophilic (HBr). Tetraethylorthosilicate wa...
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ftdoajarticles:oai:doaj.org/article:fa5216052a4b4b64a05ac3288d754c55 2023-05-15T13:33:52+02:00 ESTUDO DA IMOBILIZAÇÃO DE LIPASE EM SÍLICA OBTIDA PELA TÉCNICA SOL-GEL Aline Matuella Moreira Ficanha Nádia Ligianara D. Nyari Katarine Levandoski Marcelo Luis Mignoni Rogério Marcos Dallago 2015-03-01T00:00:00Z https://doi.org/10.5935/0100-4042.20150027 https://doaj.org/article/fa5216052a4b4b64a05ac3288d754c55 EN ES PT eng spa por Sociedade Brasileira de Química http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422015000300364&lng=en&tlng=en https://doaj.org/toc/1678-7064 1678-7064 doi:10.5935/0100-4042.20150027 https://doaj.org/article/fa5216052a4b4b64a05ac3288d754c55 Química Nova, Vol 38, Iss 3, Pp 364-369 (2015) immobilization lipase sol-gel PEG 1500 esterification Chemistry QD1-999 article 2015 ftdoajarticles https://doi.org/10.5935/0100-4042.20150027 2022-12-30T21:18:51Z The objective of this work was the immobilization of the enzyme Candida antarctica lipase B (CAL B) using the sol-gel method of immobilization and three different initiators of the polymerization reaction: one acid (HCl), one basic (NH4OH) and the other nucleophilic (HBr). Tetraethylorthosilicate was used as the silica precursor. The influence of the additive PEG 1500 on immobilization was assessed. The efficiency of the process was evaluated considering the esterification activity of the xerogels. The immobilization process provided enhanced thermal stability, storage and operational aspects relative to the free enzyme. Storage temperature proved one of the main variables to be considered in the process, with the xerogels stored under refrigeration showing better results in terms of residual activity (nearly 200 days with ≥ 90% residual activity of basic and nucleophilic xerogels) when compared with storage at ambient temperature (nearly 40 days). The results demonstrated the possibility of reuse of derivatives and a greater number of cycles (nine), considering a residual activity of 50%. Article in Journal/Newspaper Antarc* Antarctica Directory of Open Access Journals: DOAJ Articles Química Nova |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English Spanish Portuguese |
topic |
immobilization lipase sol-gel PEG 1500 esterification Chemistry QD1-999 |
spellingShingle |
immobilization lipase sol-gel PEG 1500 esterification Chemistry QD1-999 Aline Matuella Moreira Ficanha Nádia Ligianara D. Nyari Katarine Levandoski Marcelo Luis Mignoni Rogério Marcos Dallago ESTUDO DA IMOBILIZAÇÃO DE LIPASE EM SÍLICA OBTIDA PELA TÉCNICA SOL-GEL |
topic_facet |
immobilization lipase sol-gel PEG 1500 esterification Chemistry QD1-999 |
description |
The objective of this work was the immobilization of the enzyme Candida antarctica lipase B (CAL B) using the sol-gel method of immobilization and three different initiators of the polymerization reaction: one acid (HCl), one basic (NH4OH) and the other nucleophilic (HBr). Tetraethylorthosilicate was used as the silica precursor. The influence of the additive PEG 1500 on immobilization was assessed. The efficiency of the process was evaluated considering the esterification activity of the xerogels. The immobilization process provided enhanced thermal stability, storage and operational aspects relative to the free enzyme. Storage temperature proved one of the main variables to be considered in the process, with the xerogels stored under refrigeration showing better results in terms of residual activity (nearly 200 days with ≥ 90% residual activity of basic and nucleophilic xerogels) when compared with storage at ambient temperature (nearly 40 days). The results demonstrated the possibility of reuse of derivatives and a greater number of cycles (nine), considering a residual activity of 50%. |
format |
Article in Journal/Newspaper |
author |
Aline Matuella Moreira Ficanha Nádia Ligianara D. Nyari Katarine Levandoski Marcelo Luis Mignoni Rogério Marcos Dallago |
author_facet |
Aline Matuella Moreira Ficanha Nádia Ligianara D. Nyari Katarine Levandoski Marcelo Luis Mignoni Rogério Marcos Dallago |
author_sort |
Aline Matuella Moreira Ficanha |
title |
ESTUDO DA IMOBILIZAÇÃO DE LIPASE EM SÍLICA OBTIDA PELA TÉCNICA SOL-GEL |
title_short |
ESTUDO DA IMOBILIZAÇÃO DE LIPASE EM SÍLICA OBTIDA PELA TÉCNICA SOL-GEL |
title_full |
ESTUDO DA IMOBILIZAÇÃO DE LIPASE EM SÍLICA OBTIDA PELA TÉCNICA SOL-GEL |
title_fullStr |
ESTUDO DA IMOBILIZAÇÃO DE LIPASE EM SÍLICA OBTIDA PELA TÉCNICA SOL-GEL |
title_full_unstemmed |
ESTUDO DA IMOBILIZAÇÃO DE LIPASE EM SÍLICA OBTIDA PELA TÉCNICA SOL-GEL |
title_sort |
estudo da imobilização de lipase em sílica obtida pela técnica sol-gel |
publisher |
Sociedade Brasileira de Química |
publishDate |
2015 |
url |
https://doi.org/10.5935/0100-4042.20150027 https://doaj.org/article/fa5216052a4b4b64a05ac3288d754c55 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Química Nova, Vol 38, Iss 3, Pp 364-369 (2015) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422015000300364&lng=en&tlng=en https://doaj.org/toc/1678-7064 1678-7064 doi:10.5935/0100-4042.20150027 https://doaj.org/article/fa5216052a4b4b64a05ac3288d754c55 |
op_doi |
https://doi.org/10.5935/0100-4042.20150027 |
container_title |
Química Nova |
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1766046553172082688 |