Rickettsiae in red fox (Vulpes vulpes), marbled polecat (Vormela peregusna) and their ticks in northwestern China
Abstract Background Previously, twelve Rickettsia species were identified in ticks, fleas, sheep keds (Melophagus ovinus), bats (Pipistrellus pipistrellus) and a tick-bitten patient in the Xinjiang Uygur Autonomous Region (XUAR) in northwestern China. Here we aimed to molecularly detect rickettsial...
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ftdoajarticles:oai:doaj.org/article:fa519658ab7c40b2bd8639aa36511d3e 2023-05-15T17:59:57+02:00 Rickettsiae in red fox (Vulpes vulpes), marbled polecat (Vormela peregusna) and their ticks in northwestern China Gang Liu Shanshan Zhao Wenbo Tan Sándor Hornok Wumei Yuan Ligu Mi Suwen Wang Zhiqiang Liu Yanyan Zhang Wurelihazi Hazihan Xinli Gu Yuanzhi Wang 2021-04-01T00:00:00Z https://doi.org/10.1186/s13071-021-04718-1 https://doaj.org/article/fa519658ab7c40b2bd8639aa36511d3e EN eng BMC https://doi.org/10.1186/s13071-021-04718-1 https://doaj.org/toc/1756-3305 doi:10.1186/s13071-021-04718-1 1756-3305 https://doaj.org/article/fa519658ab7c40b2bd8639aa36511d3e Parasites & Vectors, Vol 14, Iss 1, Pp 1-6 (2021) Rickettsia Red fox Marbled polecat Ticks Northwestern China Infectious and parasitic diseases RC109-216 article 2021 ftdoajarticles https://doi.org/10.1186/s13071-021-04718-1 2022-12-31T06:11:26Z Abstract Background Previously, twelve Rickettsia species were identified in ticks, fleas, sheep keds (Melophagus ovinus), bats (Pipistrellus pipistrellus) and a tick-bitten patient in the Xinjiang Uygur Autonomous Region (XUAR) in northwestern China. Here we aimed to molecularly detect rickettsial agents in red fox (Vulpes vulpes), marbled polecat (Vormela peregusna) and their ticks. Methods During 2018–2019, 12 red foxes, one marbled polecat and their ticks were sampled in two counties and a city of the XUAR. The heart, liver, spleen, lung and kidney of these 13 carnivores were dissected, followed by DNA extraction. Hard ticks were identified both morphologically and molecularly. All samples were examined for the presence of rickettsiae by amplifying four genetic markers (17-kDa, gltA, ompA, sca1). Results A total of 26 adult ticks and 28 nymphs (38 Ixodes canisuga, nine Ixodes kaiseri, six Haemaphysalis erinacei and one Dermacentor marginatus) were collected from red foxes, and four Ha. erinacei ticks were removed from the marbled polecat. Analysis of cytochrome c oxidase subunit I (COI) gene sequences indicated that 2–32 nucleotides differed between I. canisuga, I. kaiseri and Ha. erinacei from northwestern China and Europe. Rickettsia raoultii was detected in three red foxes, Candidatus Rickettsia barbariae in a red fox, Rickettsia sibirica in a red fox and a marbled polecat, and R. raoultii in two tick species (I. canisuga and D. marginatus). Conclusions To the best of our knowledge, I. canisuga and I. kaiseri have not been previously reported from red foxes in China. The DNA of R. sibirica and R. raoultii was detected for the first time in the organs of red foxes, and R. sibirica in the organs of a marbled polecat. This is also the first molecular evidence for the presence of R. raoultii in I. canisuga. Our findings expand the range of tick-borne pathogens in wildlife species and associated ticks in China. Article in Journal/Newspaper Pipistrellus pipistrellus Directory of Open Access Journals: DOAJ Articles Parasites & Vectors 14 1 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Rickettsia Red fox Marbled polecat Ticks Northwestern China Infectious and parasitic diseases RC109-216 |
spellingShingle |
Rickettsia Red fox Marbled polecat Ticks Northwestern China Infectious and parasitic diseases RC109-216 Gang Liu Shanshan Zhao Wenbo Tan Sándor Hornok Wumei Yuan Ligu Mi Suwen Wang Zhiqiang Liu Yanyan Zhang Wurelihazi Hazihan Xinli Gu Yuanzhi Wang Rickettsiae in red fox (Vulpes vulpes), marbled polecat (Vormela peregusna) and their ticks in northwestern China |
topic_facet |
Rickettsia Red fox Marbled polecat Ticks Northwestern China Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Previously, twelve Rickettsia species were identified in ticks, fleas, sheep keds (Melophagus ovinus), bats (Pipistrellus pipistrellus) and a tick-bitten patient in the Xinjiang Uygur Autonomous Region (XUAR) in northwestern China. Here we aimed to molecularly detect rickettsial agents in red fox (Vulpes vulpes), marbled polecat (Vormela peregusna) and their ticks. Methods During 2018–2019, 12 red foxes, one marbled polecat and their ticks were sampled in two counties and a city of the XUAR. The heart, liver, spleen, lung and kidney of these 13 carnivores were dissected, followed by DNA extraction. Hard ticks were identified both morphologically and molecularly. All samples were examined for the presence of rickettsiae by amplifying four genetic markers (17-kDa, gltA, ompA, sca1). Results A total of 26 adult ticks and 28 nymphs (38 Ixodes canisuga, nine Ixodes kaiseri, six Haemaphysalis erinacei and one Dermacentor marginatus) were collected from red foxes, and four Ha. erinacei ticks were removed from the marbled polecat. Analysis of cytochrome c oxidase subunit I (COI) gene sequences indicated that 2–32 nucleotides differed between I. canisuga, I. kaiseri and Ha. erinacei from northwestern China and Europe. Rickettsia raoultii was detected in three red foxes, Candidatus Rickettsia barbariae in a red fox, Rickettsia sibirica in a red fox and a marbled polecat, and R. raoultii in two tick species (I. canisuga and D. marginatus). Conclusions To the best of our knowledge, I. canisuga and I. kaiseri have not been previously reported from red foxes in China. The DNA of R. sibirica and R. raoultii was detected for the first time in the organs of red foxes, and R. sibirica in the organs of a marbled polecat. This is also the first molecular evidence for the presence of R. raoultii in I. canisuga. Our findings expand the range of tick-borne pathogens in wildlife species and associated ticks in China. |
format |
Article in Journal/Newspaper |
author |
Gang Liu Shanshan Zhao Wenbo Tan Sándor Hornok Wumei Yuan Ligu Mi Suwen Wang Zhiqiang Liu Yanyan Zhang Wurelihazi Hazihan Xinli Gu Yuanzhi Wang |
author_facet |
Gang Liu Shanshan Zhao Wenbo Tan Sándor Hornok Wumei Yuan Ligu Mi Suwen Wang Zhiqiang Liu Yanyan Zhang Wurelihazi Hazihan Xinli Gu Yuanzhi Wang |
author_sort |
Gang Liu |
title |
Rickettsiae in red fox (Vulpes vulpes), marbled polecat (Vormela peregusna) and their ticks in northwestern China |
title_short |
Rickettsiae in red fox (Vulpes vulpes), marbled polecat (Vormela peregusna) and their ticks in northwestern China |
title_full |
Rickettsiae in red fox (Vulpes vulpes), marbled polecat (Vormela peregusna) and their ticks in northwestern China |
title_fullStr |
Rickettsiae in red fox (Vulpes vulpes), marbled polecat (Vormela peregusna) and their ticks in northwestern China |
title_full_unstemmed |
Rickettsiae in red fox (Vulpes vulpes), marbled polecat (Vormela peregusna) and their ticks in northwestern China |
title_sort |
rickettsiae in red fox (vulpes vulpes), marbled polecat (vormela peregusna) and their ticks in northwestern china |
publisher |
BMC |
publishDate |
2021 |
url |
https://doi.org/10.1186/s13071-021-04718-1 https://doaj.org/article/fa519658ab7c40b2bd8639aa36511d3e |
genre |
Pipistrellus pipistrellus |
genre_facet |
Pipistrellus pipistrellus |
op_source |
Parasites & Vectors, Vol 14, Iss 1, Pp 1-6 (2021) |
op_relation |
https://doi.org/10.1186/s13071-021-04718-1 https://doaj.org/toc/1756-3305 doi:10.1186/s13071-021-04718-1 1756-3305 https://doaj.org/article/fa519658ab7c40b2bd8639aa36511d3e |
op_doi |
https://doi.org/10.1186/s13071-021-04718-1 |
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Parasites & Vectors |
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