miR-19b-3p regulated by estrogen controls lipid synthesis through targeting MSMO1 and ELOVL5 in LMH cells
ABSTRACT: miR-19b-3p is reported to undertake various biological role, while its function and action mechanism in chicken hepatic lipid metabolism is unclear. Conservation analysis and tissue expression pattern of miR-19b-3p and its target gene were evaluated, respectively. Dual luciferase reporter...
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Online Access: | https://doi.org/10.1016/j.psj.2023.103200 https://doaj.org/article/fa093a88f9ba4142871c9792638453bd |
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ftdoajarticles:oai:doaj.org/article:fa093a88f9ba4142871c9792638453bd 2024-02-04T10:00:30+01:00 miR-19b-3p regulated by estrogen controls lipid synthesis through targeting MSMO1 and ELOVL5 in LMH cells Qihui Jia Yuzhu Cao Mengmeng Zhang Yuxin Xing Tian Xia Yulong Guo Yaxin Yue Xin Li Xiaojun Liu Yanhua Zhang Donghua Li Zhuanjian Li Yadong Tian Xiangtao Kang Hong Li 2024-01-01T00:00:00Z https://doi.org/10.1016/j.psj.2023.103200 https://doaj.org/article/fa093a88f9ba4142871c9792638453bd EN eng Elsevier http://www.sciencedirect.com/science/article/pii/S0032579123007198 https://doaj.org/toc/0032-5791 0032-5791 doi:10.1016/j.psj.2023.103200 https://doaj.org/article/fa093a88f9ba4142871c9792638453bd Poultry Science, Vol 103, Iss 1, Pp 103200- (2024) miR-19b-3p ELOVL5 MSMO1 estrogen lipid metabolism Animal culture SF1-1100 article 2024 ftdoajarticles https://doi.org/10.1016/j.psj.2023.103200 2024-01-07T01:41:30Z ABSTRACT: miR-19b-3p is reported to undertake various biological role, while its function and action mechanism in chicken hepatic lipid metabolism is unclear. Conservation analysis and tissue expression pattern of miR-19b-3p and its target gene were evaluated, respectively. Dual luciferase reporter system and Western blot technologies were adopted to validate miR-19b-3p target gene. Overexpression and knockdown assays were done to explore the biological functions of miR-19b-3p and target gene in Leghorn Male Hepatoma cell line (LMH). Regulatory approaches of estrogen on miR-19b-3p and target gene expressions are analyzed through site-directed mutation combined with estrogen receptors antagonist treatment assays. The results showed that chicken miR-19b-3p mature sequences are highly conserved among Capra hircus, Columba livia, Rattus norvegicus, Mus musculus, Cricetulus griseus, Danio rerio, Danio novaehollandiae, Orycodylus porosus, Crocodylus porosus, Gadus morhua, and widely expressed in lung, ovary, spleen, duodenum, kidney, heart, liver, leg muscle, and pectoral muscle tissues. miR-19b-3p could significantly increase intracellular triglyceride (TG) content and decrease intracellular cholesterol (TC) content via targeting methylsterol monooxygenase 1 (MSMO1) and elongase of very long chain fatty acids 5 (ELOVL5), which are highly conserved among species, in both mRNA and protein levels. Estrogen could inhibit miR-19b-3p expression, but directly promoted MSMO1 transcription via estrogen receptor α (ERα) and indirectly regulated ELOVL5 expression at the transcription level. Meanwhile, estrogen could also upregulate MSMO1 and ELOVL5 expression through inhibiting miR-19b-3p expression at the post-transcription level. Taken together, these results highlight the role and regulatory mechanism of miR-19b-3p in hepatic lipid metabolism in chicken, and might produce useful comparative information for human obesity studies and biomedical research. Article in Journal/Newspaper Gadus morhua Directory of Open Access Journals: DOAJ Articles Poultry Science 103 1 103200 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
miR-19b-3p ELOVL5 MSMO1 estrogen lipid metabolism Animal culture SF1-1100 |
spellingShingle |
miR-19b-3p ELOVL5 MSMO1 estrogen lipid metabolism Animal culture SF1-1100 Qihui Jia Yuzhu Cao Mengmeng Zhang Yuxin Xing Tian Xia Yulong Guo Yaxin Yue Xin Li Xiaojun Liu Yanhua Zhang Donghua Li Zhuanjian Li Yadong Tian Xiangtao Kang Hong Li miR-19b-3p regulated by estrogen controls lipid synthesis through targeting MSMO1 and ELOVL5 in LMH cells |
topic_facet |
miR-19b-3p ELOVL5 MSMO1 estrogen lipid metabolism Animal culture SF1-1100 |
description |
ABSTRACT: miR-19b-3p is reported to undertake various biological role, while its function and action mechanism in chicken hepatic lipid metabolism is unclear. Conservation analysis and tissue expression pattern of miR-19b-3p and its target gene were evaluated, respectively. Dual luciferase reporter system and Western blot technologies were adopted to validate miR-19b-3p target gene. Overexpression and knockdown assays were done to explore the biological functions of miR-19b-3p and target gene in Leghorn Male Hepatoma cell line (LMH). Regulatory approaches of estrogen on miR-19b-3p and target gene expressions are analyzed through site-directed mutation combined with estrogen receptors antagonist treatment assays. The results showed that chicken miR-19b-3p mature sequences are highly conserved among Capra hircus, Columba livia, Rattus norvegicus, Mus musculus, Cricetulus griseus, Danio rerio, Danio novaehollandiae, Orycodylus porosus, Crocodylus porosus, Gadus morhua, and widely expressed in lung, ovary, spleen, duodenum, kidney, heart, liver, leg muscle, and pectoral muscle tissues. miR-19b-3p could significantly increase intracellular triglyceride (TG) content and decrease intracellular cholesterol (TC) content via targeting methylsterol monooxygenase 1 (MSMO1) and elongase of very long chain fatty acids 5 (ELOVL5), which are highly conserved among species, in both mRNA and protein levels. Estrogen could inhibit miR-19b-3p expression, but directly promoted MSMO1 transcription via estrogen receptor α (ERα) and indirectly regulated ELOVL5 expression at the transcription level. Meanwhile, estrogen could also upregulate MSMO1 and ELOVL5 expression through inhibiting miR-19b-3p expression at the post-transcription level. Taken together, these results highlight the role and regulatory mechanism of miR-19b-3p in hepatic lipid metabolism in chicken, and might produce useful comparative information for human obesity studies and biomedical research. |
format |
Article in Journal/Newspaper |
author |
Qihui Jia Yuzhu Cao Mengmeng Zhang Yuxin Xing Tian Xia Yulong Guo Yaxin Yue Xin Li Xiaojun Liu Yanhua Zhang Donghua Li Zhuanjian Li Yadong Tian Xiangtao Kang Hong Li |
author_facet |
Qihui Jia Yuzhu Cao Mengmeng Zhang Yuxin Xing Tian Xia Yulong Guo Yaxin Yue Xin Li Xiaojun Liu Yanhua Zhang Donghua Li Zhuanjian Li Yadong Tian Xiangtao Kang Hong Li |
author_sort |
Qihui Jia |
title |
miR-19b-3p regulated by estrogen controls lipid synthesis through targeting MSMO1 and ELOVL5 in LMH cells |
title_short |
miR-19b-3p regulated by estrogen controls lipid synthesis through targeting MSMO1 and ELOVL5 in LMH cells |
title_full |
miR-19b-3p regulated by estrogen controls lipid synthesis through targeting MSMO1 and ELOVL5 in LMH cells |
title_fullStr |
miR-19b-3p regulated by estrogen controls lipid synthesis through targeting MSMO1 and ELOVL5 in LMH cells |
title_full_unstemmed |
miR-19b-3p regulated by estrogen controls lipid synthesis through targeting MSMO1 and ELOVL5 in LMH cells |
title_sort |
mir-19b-3p regulated by estrogen controls lipid synthesis through targeting msmo1 and elovl5 in lmh cells |
publisher |
Elsevier |
publishDate |
2024 |
url |
https://doi.org/10.1016/j.psj.2023.103200 https://doaj.org/article/fa093a88f9ba4142871c9792638453bd |
genre |
Gadus morhua |
genre_facet |
Gadus morhua |
op_source |
Poultry Science, Vol 103, Iss 1, Pp 103200- (2024) |
op_relation |
http://www.sciencedirect.com/science/article/pii/S0032579123007198 https://doaj.org/toc/0032-5791 0032-5791 doi:10.1016/j.psj.2023.103200 https://doaj.org/article/fa093a88f9ba4142871c9792638453bd |
op_doi |
https://doi.org/10.1016/j.psj.2023.103200 |
container_title |
Poultry Science |
container_volume |
103 |
container_issue |
1 |
container_start_page |
103200 |
_version_ |
1789965841818714112 |