Restructuring of epibacterial communities on Fucus vesiculosus forma mytili in response to elevated pCO2 and increased temperature levels
Marine multicellular organisms in composition with their associated microbiota – representing metaorganisms – are confronted with constantly changing environmental conditions. In 2110, the seawater temperature is predicted to be increased by approximately 5 °C, and the atmospheric carbon dioxide par...
Published in: | Frontiers in Microbiology |
---|---|
Main Authors: | , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Frontiers Media S.A.
2016
|
Subjects: | |
Online Access: | https://doi.org/10.3389/fmicb.2016.00434 https://doaj.org/article/f9ccd592ec1c428a9775b9fd3e03bdfc |
id |
ftdoajarticles:oai:doaj.org/article:f9ccd592ec1c428a9775b9fd3e03bdfc |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:f9ccd592ec1c428a9775b9fd3e03bdfc 2023-05-15T17:51:49+02:00 Restructuring of epibacterial communities on Fucus vesiculosus forma mytili in response to elevated pCO2 and increased temperature levels Birte eMensch Sven Christopher Neulinger Angelika eGraiff Andreas ePansch Sven eKünzel Martin Alexander Fischer Ruth Anne Schmitz 2016-03-01T00:00:00Z https://doi.org/10.3389/fmicb.2016.00434 https://doaj.org/article/f9ccd592ec1c428a9775b9fd3e03bdfc EN eng Frontiers Media S.A. http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.00434/full https://doaj.org/toc/1664-302X 1664-302X doi:10.3389/fmicb.2016.00434 https://doaj.org/article/f9ccd592ec1c428a9775b9fd3e03bdfc Frontiers in Microbiology, Vol 7 (2016) Global Warming ocean acidification 16S rDNA pCO2 mesocosm Epibacteria Microbiology QR1-502 article 2016 ftdoajarticles https://doi.org/10.3389/fmicb.2016.00434 2022-12-31T12:32:34Z Marine multicellular organisms in composition with their associated microbiota – representing metaorganisms – are confronted with constantly changing environmental conditions. In 2110, the seawater temperature is predicted to be increased by approximately 5 °C, and the atmospheric carbon dioxide partial pressure (pCO2) is expected to reach approximately 1,000 ppm. In order to assess the response of marine metaorganisms to global changes, e.g. by effects on host-microbe interactions, we evaluated the response of epibacterial communities associated with Fucus vesiculosus forma mytili (F. mytili) to future climate conditions. During an 11-week lasting mesocosm experiment on the island of Sylt (Germany) in spring 2014, North Sea F. mytili individuals were exposed to elevated pCO2 (1,000 ppm) and increased temperature levels (∆+5 °C). Both abiotic factors were tested for single and combined effects on the epibacterial community composition over time, with three replicates per treatment. The respective community structures of bacterial consortia associated to the surface of F. mytili were analyzed by Illumina MiSeq 16S rDNA amplicon sequencing after 0, 4, 8 and 11 weeks of treatment (in total 96 samples). The results demonstrated that the epibacterial community structure was strongly affected by temperature, but only weakly by elevated pCO2. No interaction effect of both factors was observed in the combined treatment. We identified several indicator operational taxonomic units (iOTUs) that were strongly influenced by the respective experimental factors. An OTU association network analysis revealed that relationships between OTUs were mainly governed by habitat. Overall, this study contributes to a better understanding of how epibacterial communities associated with F. mytili may adapt to future changes in seawater acidity and temperature, ultimately with potential consequences for host-microbe interactions. Article in Journal/Newspaper Ocean acidification Directory of Open Access Journals: DOAJ Articles Frontiers in Microbiology 7 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Global Warming ocean acidification 16S rDNA pCO2 mesocosm Epibacteria Microbiology QR1-502 |
spellingShingle |
Global Warming ocean acidification 16S rDNA pCO2 mesocosm Epibacteria Microbiology QR1-502 Birte eMensch Sven Christopher Neulinger Angelika eGraiff Andreas ePansch Sven eKünzel Martin Alexander Fischer Ruth Anne Schmitz Restructuring of epibacterial communities on Fucus vesiculosus forma mytili in response to elevated pCO2 and increased temperature levels |
topic_facet |
Global Warming ocean acidification 16S rDNA pCO2 mesocosm Epibacteria Microbiology QR1-502 |
description |
Marine multicellular organisms in composition with their associated microbiota – representing metaorganisms – are confronted with constantly changing environmental conditions. In 2110, the seawater temperature is predicted to be increased by approximately 5 °C, and the atmospheric carbon dioxide partial pressure (pCO2) is expected to reach approximately 1,000 ppm. In order to assess the response of marine metaorganisms to global changes, e.g. by effects on host-microbe interactions, we evaluated the response of epibacterial communities associated with Fucus vesiculosus forma mytili (F. mytili) to future climate conditions. During an 11-week lasting mesocosm experiment on the island of Sylt (Germany) in spring 2014, North Sea F. mytili individuals were exposed to elevated pCO2 (1,000 ppm) and increased temperature levels (∆+5 °C). Both abiotic factors were tested for single and combined effects on the epibacterial community composition over time, with three replicates per treatment. The respective community structures of bacterial consortia associated to the surface of F. mytili were analyzed by Illumina MiSeq 16S rDNA amplicon sequencing after 0, 4, 8 and 11 weeks of treatment (in total 96 samples). The results demonstrated that the epibacterial community structure was strongly affected by temperature, but only weakly by elevated pCO2. No interaction effect of both factors was observed in the combined treatment. We identified several indicator operational taxonomic units (iOTUs) that were strongly influenced by the respective experimental factors. An OTU association network analysis revealed that relationships between OTUs were mainly governed by habitat. Overall, this study contributes to a better understanding of how epibacterial communities associated with F. mytili may adapt to future changes in seawater acidity and temperature, ultimately with potential consequences for host-microbe interactions. |
format |
Article in Journal/Newspaper |
author |
Birte eMensch Sven Christopher Neulinger Angelika eGraiff Andreas ePansch Sven eKünzel Martin Alexander Fischer Ruth Anne Schmitz |
author_facet |
Birte eMensch Sven Christopher Neulinger Angelika eGraiff Andreas ePansch Sven eKünzel Martin Alexander Fischer Ruth Anne Schmitz |
author_sort |
Birte eMensch |
title |
Restructuring of epibacterial communities on Fucus vesiculosus forma mytili in response to elevated pCO2 and increased temperature levels |
title_short |
Restructuring of epibacterial communities on Fucus vesiculosus forma mytili in response to elevated pCO2 and increased temperature levels |
title_full |
Restructuring of epibacterial communities on Fucus vesiculosus forma mytili in response to elevated pCO2 and increased temperature levels |
title_fullStr |
Restructuring of epibacterial communities on Fucus vesiculosus forma mytili in response to elevated pCO2 and increased temperature levels |
title_full_unstemmed |
Restructuring of epibacterial communities on Fucus vesiculosus forma mytili in response to elevated pCO2 and increased temperature levels |
title_sort |
restructuring of epibacterial communities on fucus vesiculosus forma mytili in response to elevated pco2 and increased temperature levels |
publisher |
Frontiers Media S.A. |
publishDate |
2016 |
url |
https://doi.org/10.3389/fmicb.2016.00434 https://doaj.org/article/f9ccd592ec1c428a9775b9fd3e03bdfc |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_source |
Frontiers in Microbiology, Vol 7 (2016) |
op_relation |
http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.00434/full https://doaj.org/toc/1664-302X 1664-302X doi:10.3389/fmicb.2016.00434 https://doaj.org/article/f9ccd592ec1c428a9775b9fd3e03bdfc |
op_doi |
https://doi.org/10.3389/fmicb.2016.00434 |
container_title |
Frontiers in Microbiology |
container_volume |
7 |
_version_ |
1766159083916754944 |