Contribution to the laboratory diagnosis of human cryptosporidiosis Contribuição ao diagnóstico laboratorial da criptosporidiose humana
Human cryptosporidiosis is an infection caused by Cryptosporidium spp, a coccidial protozoan of emergencial pathogenicity and responsible for severe and prostrating watery diarrhea, mainly in immunocompromised patients. Smears of stools submitted to concentration and staining by carbol fuchsin techn...
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ftdoajarticles:oai:doaj.org/article:f9c8dce208014b4a88b1743c3a0d4eb5 2024-09-09T19:28:12+00:00 Contribution to the laboratory diagnosis of human cryptosporidiosis Contribuição ao diagnóstico laboratorial da criptosporidiose humana Isabel MARTINEZ Francisco Miguel BELDA NETO 2001-04-01T00:00:00Z https://doi.org/10.1590/S0036-46652001000200005 https://doaj.org/article/f9c8dce208014b4a88b1743c3a0d4eb5 EN eng Universidade de São Paulo (USP) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652001000200005 https://doaj.org/toc/0036-4665 https://doaj.org/toc/1678-9946 doi:10.1590/S0036-46652001000200005 0036-4665 1678-9946 https://doaj.org/article/f9c8dce208014b4a88b1743c3a0d4eb5 Revista do Instituto de Medicina Tropical de São Paulo, Vol 43, Iss 2, Pp 79-82 (2001) Cryptosporidium spp AIDS diagnosis Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2001 ftdoajarticles https://doi.org/10.1590/S0036-46652001000200005 2024-08-05T17:49:30Z Human cryptosporidiosis is an infection caused by Cryptosporidium spp, a coccidial protozoan of emergencial pathogenicity and responsible for severe and prostrating watery diarrhea, mainly in immunocompromised patients. Smears of stools submitted to concentration and staining by carbol fuchsin technique has been used in our laboratory as a diagnostic procedure for cryptosporidiosis. The long time observing the smears in the microscope due to the small size of the forms and the low contrast of the staining led us to introduce some modifications in the original protocol for the acid-fast staining. The smears were treated with the carbol fuchsin solution for 3 minutes as recommended by LENNETTE et al., 1985 and the solution of the ethyl alcohol 70%-chloride acid 0.5% was used instead of the ethyl alcohol-sulfuric acid 5% recommended by HENRIKSEN & POHLENZ, 1981. Smears were treated with the discoloration solution for 2 minutes. These modifications promoted a better washing out of the excess of carbol fuchsin therefore increasing the dye efficiency. In such conditions, the visualization of protozoan oocysts on the slides examined became easier. They were observed by the contrast of their intense pink-reddish pigmentation against the blue background. It's worthwhile to emphasize that these modifications offer advantages when time and accuracy are concerned. A criptosporidiose humana é uma infecção causada pelo Cryptosporidium sp, um protozoário coccídio de patogenicidade emergente e responsável por severa e prostrante diarréia aquosa em humanos, principalmente em indivíduos imunodeprimidos. O diagnóstico, feito através da utilização de esfregaços de fezes submetidos a técnicas de concentração e coloração específica pela fucsina-carbólica tem oferecido bons resultados em nosso laboratório. Tendo em vista o longo tempo despendido para a observação dos esfregaços considerando-se a pequenez das formas e o contraste da coloração, realizamos modificações no procedimento técnico da coloração ácido-resistente que ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Revista do Instituto de Medicina Tropical de São Paulo 43 2 79 82 |
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Cryptosporidium spp AIDS diagnosis Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
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Cryptosporidium spp AIDS diagnosis Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Isabel MARTINEZ Francisco Miguel BELDA NETO Contribution to the laboratory diagnosis of human cryptosporidiosis Contribuição ao diagnóstico laboratorial da criptosporidiose humana |
topic_facet |
Cryptosporidium spp AIDS diagnosis Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Human cryptosporidiosis is an infection caused by Cryptosporidium spp, a coccidial protozoan of emergencial pathogenicity and responsible for severe and prostrating watery diarrhea, mainly in immunocompromised patients. Smears of stools submitted to concentration and staining by carbol fuchsin technique has been used in our laboratory as a diagnostic procedure for cryptosporidiosis. The long time observing the smears in the microscope due to the small size of the forms and the low contrast of the staining led us to introduce some modifications in the original protocol for the acid-fast staining. The smears were treated with the carbol fuchsin solution for 3 minutes as recommended by LENNETTE et al., 1985 and the solution of the ethyl alcohol 70%-chloride acid 0.5% was used instead of the ethyl alcohol-sulfuric acid 5% recommended by HENRIKSEN & POHLENZ, 1981. Smears were treated with the discoloration solution for 2 minutes. These modifications promoted a better washing out of the excess of carbol fuchsin therefore increasing the dye efficiency. In such conditions, the visualization of protozoan oocysts on the slides examined became easier. They were observed by the contrast of their intense pink-reddish pigmentation against the blue background. It's worthwhile to emphasize that these modifications offer advantages when time and accuracy are concerned. A criptosporidiose humana é uma infecção causada pelo Cryptosporidium sp, um protozoário coccídio de patogenicidade emergente e responsável por severa e prostrante diarréia aquosa em humanos, principalmente em indivíduos imunodeprimidos. O diagnóstico, feito através da utilização de esfregaços de fezes submetidos a técnicas de concentração e coloração específica pela fucsina-carbólica tem oferecido bons resultados em nosso laboratório. Tendo em vista o longo tempo despendido para a observação dos esfregaços considerando-se a pequenez das formas e o contraste da coloração, realizamos modificações no procedimento técnico da coloração ácido-resistente que ... |
format |
Article in Journal/Newspaper |
author |
Isabel MARTINEZ Francisco Miguel BELDA NETO |
author_facet |
Isabel MARTINEZ Francisco Miguel BELDA NETO |
author_sort |
Isabel MARTINEZ |
title |
Contribution to the laboratory diagnosis of human cryptosporidiosis Contribuição ao diagnóstico laboratorial da criptosporidiose humana |
title_short |
Contribution to the laboratory diagnosis of human cryptosporidiosis Contribuição ao diagnóstico laboratorial da criptosporidiose humana |
title_full |
Contribution to the laboratory diagnosis of human cryptosporidiosis Contribuição ao diagnóstico laboratorial da criptosporidiose humana |
title_fullStr |
Contribution to the laboratory diagnosis of human cryptosporidiosis Contribuição ao diagnóstico laboratorial da criptosporidiose humana |
title_full_unstemmed |
Contribution to the laboratory diagnosis of human cryptosporidiosis Contribuição ao diagnóstico laboratorial da criptosporidiose humana |
title_sort |
contribution to the laboratory diagnosis of human cryptosporidiosis contribuição ao diagnóstico laboratorial da criptosporidiose humana |
publisher |
Universidade de São Paulo (USP) |
publishDate |
2001 |
url |
https://doi.org/10.1590/S0036-46652001000200005 https://doaj.org/article/f9c8dce208014b4a88b1743c3a0d4eb5 |
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Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
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Revista do Instituto de Medicina Tropical de São Paulo, Vol 43, Iss 2, Pp 79-82 (2001) |
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http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652001000200005 https://doaj.org/toc/0036-4665 https://doaj.org/toc/1678-9946 doi:10.1590/S0036-46652001000200005 0036-4665 1678-9946 https://doaj.org/article/f9c8dce208014b4a88b1743c3a0d4eb5 |
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https://doi.org/10.1590/S0036-46652001000200005 |
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Revista do Instituto de Medicina Tropical de São Paulo |
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