Molecular Detection and Phylogenetic Analysis of Canine Distemper Virus in Marsican Brown Bear ( Ursus arctos marsicanus )
In this paper, we report the first molecular detection of the canine distemper virus in the Marsican brown bear ( Ursus arctos marsicanus ). Three subadults and one adult were live-trapped and checked for the main viral pathogens responsible for infectious diseases in this species. The four bears we...
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ftdoajarticles:oai:doaj.org/article:f99caa7af8bf4829b0f19a86be563196 2024-01-07T09:47:10+01:00 Molecular Detection and Phylogenetic Analysis of Canine Distemper Virus in Marsican Brown Bear ( Ursus arctos marsicanus ) Cristina Esmeralda Di Francesco Camilla Smoglica Vincenza Di Pirro Federica Cafini Leonardo Gentile Fulvio Marsilio 2022-07-01T00:00:00Z https://doi.org/10.3390/ani12141826 https://doaj.org/article/f99caa7af8bf4829b0f19a86be563196 EN eng MDPI AG https://www.mdpi.com/2076-2615/12/14/1826 https://doaj.org/toc/2076-2615 doi:10.3390/ani12141826 2076-2615 https://doaj.org/article/f99caa7af8bf4829b0f19a86be563196 Animals, Vol 12, Iss 14, p 1826 (2022) Marsican brown bear canine distemper virus H gene phylogenetic analysis wildlife lineage Veterinary medicine SF600-1100 Zoology QL1-991 article 2022 ftdoajarticles https://doi.org/10.3390/ani12141826 2023-12-10T01:43:27Z In this paper, we report the first molecular detection of the canine distemper virus in the Marsican brown bear ( Ursus arctos marsicanus ). Three subadults and one adult were live-trapped and checked for the main viral pathogens responsible for infectious diseases in this species. The four bears were found to be negative for all investigated viruses except for one, which resulted in a positive outcome for CDV by means of RT-PCR targeting fragments of viral N and H genes. The sequence analysis revealed the specificity of amplicons for the Europe Wildlife lineage of CDV, the same viral strain recovered from three foxes and two unvaccinated dogs coming from the same territories where the positive bear was captured. These results confirm the receptivity of Marsican brown bear for CDV, apparently without any pathological consequences for the positive animal, and suggest the presence in the studied area of a unique wild host-adapted lineage of the virus, able to spread in domestic animals, too. In this respect, continuous and specifically targeted surveillance systems are necessary in order to highlight any changes in the epidemiology of the infection in the territories where the Marsican brown bear lives, along with a more effective vaccination program for domestic dogs co-existing with this endangered species. Article in Journal/Newspaper Ursus arctos Directory of Open Access Journals: DOAJ Articles Animals 12 14 1826 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Marsican brown bear canine distemper virus H gene phylogenetic analysis wildlife lineage Veterinary medicine SF600-1100 Zoology QL1-991 |
spellingShingle |
Marsican brown bear canine distemper virus H gene phylogenetic analysis wildlife lineage Veterinary medicine SF600-1100 Zoology QL1-991 Cristina Esmeralda Di Francesco Camilla Smoglica Vincenza Di Pirro Federica Cafini Leonardo Gentile Fulvio Marsilio Molecular Detection and Phylogenetic Analysis of Canine Distemper Virus in Marsican Brown Bear ( Ursus arctos marsicanus ) |
topic_facet |
Marsican brown bear canine distemper virus H gene phylogenetic analysis wildlife lineage Veterinary medicine SF600-1100 Zoology QL1-991 |
description |
In this paper, we report the first molecular detection of the canine distemper virus in the Marsican brown bear ( Ursus arctos marsicanus ). Three subadults and one adult were live-trapped and checked for the main viral pathogens responsible for infectious diseases in this species. The four bears were found to be negative for all investigated viruses except for one, which resulted in a positive outcome for CDV by means of RT-PCR targeting fragments of viral N and H genes. The sequence analysis revealed the specificity of amplicons for the Europe Wildlife lineage of CDV, the same viral strain recovered from three foxes and two unvaccinated dogs coming from the same territories where the positive bear was captured. These results confirm the receptivity of Marsican brown bear for CDV, apparently without any pathological consequences for the positive animal, and suggest the presence in the studied area of a unique wild host-adapted lineage of the virus, able to spread in domestic animals, too. In this respect, continuous and specifically targeted surveillance systems are necessary in order to highlight any changes in the epidemiology of the infection in the territories where the Marsican brown bear lives, along with a more effective vaccination program for domestic dogs co-existing with this endangered species. |
format |
Article in Journal/Newspaper |
author |
Cristina Esmeralda Di Francesco Camilla Smoglica Vincenza Di Pirro Federica Cafini Leonardo Gentile Fulvio Marsilio |
author_facet |
Cristina Esmeralda Di Francesco Camilla Smoglica Vincenza Di Pirro Federica Cafini Leonardo Gentile Fulvio Marsilio |
author_sort |
Cristina Esmeralda Di Francesco |
title |
Molecular Detection and Phylogenetic Analysis of Canine Distemper Virus in Marsican Brown Bear ( Ursus arctos marsicanus ) |
title_short |
Molecular Detection and Phylogenetic Analysis of Canine Distemper Virus in Marsican Brown Bear ( Ursus arctos marsicanus ) |
title_full |
Molecular Detection and Phylogenetic Analysis of Canine Distemper Virus in Marsican Brown Bear ( Ursus arctos marsicanus ) |
title_fullStr |
Molecular Detection and Phylogenetic Analysis of Canine Distemper Virus in Marsican Brown Bear ( Ursus arctos marsicanus ) |
title_full_unstemmed |
Molecular Detection and Phylogenetic Analysis of Canine Distemper Virus in Marsican Brown Bear ( Ursus arctos marsicanus ) |
title_sort |
molecular detection and phylogenetic analysis of canine distemper virus in marsican brown bear ( ursus arctos marsicanus ) |
publisher |
MDPI AG |
publishDate |
2022 |
url |
https://doi.org/10.3390/ani12141826 https://doaj.org/article/f99caa7af8bf4829b0f19a86be563196 |
genre |
Ursus arctos |
genre_facet |
Ursus arctos |
op_source |
Animals, Vol 12, Iss 14, p 1826 (2022) |
op_relation |
https://www.mdpi.com/2076-2615/12/14/1826 https://doaj.org/toc/2076-2615 doi:10.3390/ani12141826 2076-2615 https://doaj.org/article/f99caa7af8bf4829b0f19a86be563196 |
op_doi |
https://doi.org/10.3390/ani12141826 |
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Animals |
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12 |
container_issue |
14 |
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1826 |
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1787429152589086720 |