High-field NMR spectroscopy and FTICR mass spectrometry: powerful discovery tools for the molecular level characterization of marine dissolved organic matter
High-performance, non-target, high-resolution organic structural spectroscopy was applied to solid phase extracted marine dissolved organic matter (SPE-DOM) isolated from four different depths in the open South Atlantic Ocean off the Angola coast (3° E, 18° S; Angola Basin) and provided molecular le...
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ftdoajarticles:oai:doaj.org/article:f98f9504a0f349e8ad15cc7f23028802 2023-05-15T13:49:08+02:00 High-field NMR spectroscopy and FTICR mass spectrometry: powerful discovery tools for the molecular level characterization of marine dissolved organic matter N. Hertkorn M. Harir B. P. Koch B. Michalke P. Schmitt-Kopplin 2013-03-01T00:00:00Z https://doi.org/10.5194/bg-10-1583-2013 https://doaj.org/article/f98f9504a0f349e8ad15cc7f23028802 EN eng Copernicus Publications http://www.biogeosciences.net/10/1583/2013/bg-10-1583-2013.pdf https://doaj.org/toc/1726-4170 https://doaj.org/toc/1726-4189 doi:10.5194/bg-10-1583-2013 1726-4170 1726-4189 https://doaj.org/article/f98f9504a0f349e8ad15cc7f23028802 Biogeosciences, Vol 10, Iss 3, Pp 1583-1624 (2013) Ecology QH540-549.5 Life QH501-531 Geology QE1-996.5 article 2013 ftdoajarticles https://doi.org/10.5194/bg-10-1583-2013 2022-12-30T22:55:45Z High-performance, non-target, high-resolution organic structural spectroscopy was applied to solid phase extracted marine dissolved organic matter (SPE-DOM) isolated from four different depths in the open South Atlantic Ocean off the Angola coast (3° E, 18° S; Angola Basin) and provided molecular level information with extraordinary coverage and resolution. Sampling was performed at depths of 5 m (Angola Current; near-surface photic zone), 48 m (Angola Current; fluorescence maximum), 200 m (still above Antarctic Intermediate Water, AAIW; upper mesopelagic zone) and 5446 m (North Atlantic Deep Water, NADW; abyssopelagic, ~30 m above seafloor) and produced SPE-DOM with near 40% carbon yield and beneficial nuclear magnetic resonance (NMR) relaxation properties, a crucial prerequisite for the acquisition of NMR spectra with excellent resolution. 1 H and 13 C NMR spectra of all four marine SPE-DOM showed smooth bulk envelopes, reflecting intrinsic averaging from massive signal overlap, with a few percent of visibly resolved signatures and variable abundances for all major chemical environments. The abundance of singly oxygenated aliphatics and acetate derivatives in 1 H NMR spectra declined from surface to deep marine SPE-DOM, whereas C-based aliphatics and carboxyl-rich alicyclic molecules (CRAM) increased in abundance. Surface SPE-DOM contained fewer methyl esters than all other samples, likely a consequence of direct exposure to sunlight. Integration of 13 C NMR spectra revealed continual increase of carboxylic acids and ketones from surface to depth, reflecting a progressive oxygenation, with concomitant decline of carbohydrate-related substructures. Aliphatic branching increased with depth, whereas the fraction of oxygenated aliphatics declined for methine, methylene and methyl carbon. Lipids in the oldest SPE-DOM at 5446 m showed a larger share of ethyl groups and methylene carbon than observed in the other samples. Two-dimensional NMR spectra showed exceptional resolution and depicted resolved molecular ... Article in Journal/Newspaper Antarc* Antarctic NADW North Atlantic Deep Water North Atlantic South Atlantic Ocean Directory of Open Access Journals: DOAJ Articles Antarctic Biogeosciences 10 3 1583 1624 |
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Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Ecology QH540-549.5 Life QH501-531 Geology QE1-996.5 |
spellingShingle |
Ecology QH540-549.5 Life QH501-531 Geology QE1-996.5 N. Hertkorn M. Harir B. P. Koch B. Michalke P. Schmitt-Kopplin High-field NMR spectroscopy and FTICR mass spectrometry: powerful discovery tools for the molecular level characterization of marine dissolved organic matter |
topic_facet |
Ecology QH540-549.5 Life QH501-531 Geology QE1-996.5 |
description |
High-performance, non-target, high-resolution organic structural spectroscopy was applied to solid phase extracted marine dissolved organic matter (SPE-DOM) isolated from four different depths in the open South Atlantic Ocean off the Angola coast (3° E, 18° S; Angola Basin) and provided molecular level information with extraordinary coverage and resolution. Sampling was performed at depths of 5 m (Angola Current; near-surface photic zone), 48 m (Angola Current; fluorescence maximum), 200 m (still above Antarctic Intermediate Water, AAIW; upper mesopelagic zone) and 5446 m (North Atlantic Deep Water, NADW; abyssopelagic, ~30 m above seafloor) and produced SPE-DOM with near 40% carbon yield and beneficial nuclear magnetic resonance (NMR) relaxation properties, a crucial prerequisite for the acquisition of NMR spectra with excellent resolution. 1 H and 13 C NMR spectra of all four marine SPE-DOM showed smooth bulk envelopes, reflecting intrinsic averaging from massive signal overlap, with a few percent of visibly resolved signatures and variable abundances for all major chemical environments. The abundance of singly oxygenated aliphatics and acetate derivatives in 1 H NMR spectra declined from surface to deep marine SPE-DOM, whereas C-based aliphatics and carboxyl-rich alicyclic molecules (CRAM) increased in abundance. Surface SPE-DOM contained fewer methyl esters than all other samples, likely a consequence of direct exposure to sunlight. Integration of 13 C NMR spectra revealed continual increase of carboxylic acids and ketones from surface to depth, reflecting a progressive oxygenation, with concomitant decline of carbohydrate-related substructures. Aliphatic branching increased with depth, whereas the fraction of oxygenated aliphatics declined for methine, methylene and methyl carbon. Lipids in the oldest SPE-DOM at 5446 m showed a larger share of ethyl groups and methylene carbon than observed in the other samples. Two-dimensional NMR spectra showed exceptional resolution and depicted resolved molecular ... |
format |
Article in Journal/Newspaper |
author |
N. Hertkorn M. Harir B. P. Koch B. Michalke P. Schmitt-Kopplin |
author_facet |
N. Hertkorn M. Harir B. P. Koch B. Michalke P. Schmitt-Kopplin |
author_sort |
N. Hertkorn |
title |
High-field NMR spectroscopy and FTICR mass spectrometry: powerful discovery tools for the molecular level characterization of marine dissolved organic matter |
title_short |
High-field NMR spectroscopy and FTICR mass spectrometry: powerful discovery tools for the molecular level characterization of marine dissolved organic matter |
title_full |
High-field NMR spectroscopy and FTICR mass spectrometry: powerful discovery tools for the molecular level characterization of marine dissolved organic matter |
title_fullStr |
High-field NMR spectroscopy and FTICR mass spectrometry: powerful discovery tools for the molecular level characterization of marine dissolved organic matter |
title_full_unstemmed |
High-field NMR spectroscopy and FTICR mass spectrometry: powerful discovery tools for the molecular level characterization of marine dissolved organic matter |
title_sort |
high-field nmr spectroscopy and fticr mass spectrometry: powerful discovery tools for the molecular level characterization of marine dissolved organic matter |
publisher |
Copernicus Publications |
publishDate |
2013 |
url |
https://doi.org/10.5194/bg-10-1583-2013 https://doaj.org/article/f98f9504a0f349e8ad15cc7f23028802 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic NADW North Atlantic Deep Water North Atlantic South Atlantic Ocean |
genre_facet |
Antarc* Antarctic NADW North Atlantic Deep Water North Atlantic South Atlantic Ocean |
op_source |
Biogeosciences, Vol 10, Iss 3, Pp 1583-1624 (2013) |
op_relation |
http://www.biogeosciences.net/10/1583/2013/bg-10-1583-2013.pdf https://doaj.org/toc/1726-4170 https://doaj.org/toc/1726-4189 doi:10.5194/bg-10-1583-2013 1726-4170 1726-4189 https://doaj.org/article/f98f9504a0f349e8ad15cc7f23028802 |
op_doi |
https://doi.org/10.5194/bg-10-1583-2013 |
container_title |
Biogeosciences |
container_volume |
10 |
container_issue |
3 |
container_start_page |
1583 |
op_container_end_page |
1624 |
_version_ |
1766250912317177856 |