Biogenic Synthesis of Copper Nanoparticles Using Bacterial Strains Isolated from an Antarctic Consortium Associated to a Psychrophilic Marine Ciliate: Characterization and Potential Application as Antimicrobial Agents
In the last decade, metal nanoparticles (NPs) have gained significant interest in the field of biotechnology due to their unique physiochemical properties and potential uses in a wide range of applications. Metal NP synthesis using microorganisms has emerged as an eco-friendly, clean, and viable str...
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2021
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ftdoajarticles:oai:doaj.org/article:f98f3e18928e4f55854d53787226ff16 2023-05-15T14:01:37+02:00 Biogenic Synthesis of Copper Nanoparticles Using Bacterial Strains Isolated from an Antarctic Consortium Associated to a Psychrophilic Marine Ciliate: Characterization and Potential Application as Antimicrobial Agents Maria Sindhura John Joseph Amruthraj Nagoth Marco Zannotti Rita Giovannetti Alessio Mancini Kesava Priyan Ramasamy Cristina Miceli Sandra Pucciarelli 2021-05-01T00:00:00Z https://doi.org/10.3390/md19050263 https://doaj.org/article/f98f3e18928e4f55854d53787226ff16 EN eng MDPI AG https://www.mdpi.com/1660-3397/19/5/263 https://doaj.org/toc/1660-3397 doi:10.3390/md19050263 1660-3397 https://doaj.org/article/f98f3e18928e4f55854d53787226ff16 Marine Drugs, Vol 19, Iss 263, p 263 (2021) green synthesis biomaterials metal antibiotics nanotechnology Biology (General) QH301-705.5 article 2021 ftdoajarticles https://doi.org/10.3390/md19050263 2022-12-30T20:07:21Z In the last decade, metal nanoparticles (NPs) have gained significant interest in the field of biotechnology due to their unique physiochemical properties and potential uses in a wide range of applications. Metal NP synthesis using microorganisms has emerged as an eco-friendly, clean, and viable strategy alternative to chemical and physical approaches. Herein, an original and efficient route for the microbial synthesis of copper NPs using bacterial strains newly isolated from an Antarctic consortium is described. UV-visible spectra of the NPs showed a maximum absorbance in the range of 380–385 nm. Transmission electron microscopy analysis showed that these NPs are all monodispersed, spherical in nature, and well segregated without any agglomeration and with an average size of 30 nm. X-ray powder diffraction showed a polycrystalline nature and face centered cubic lattice and revealed characteristic diffraction peaks indicating the formation of CuONPs. Fourier-transform infrared spectra confirmed the presence of capping proteins on the NP surface that act as stabilizers. All CuONPs manifested antimicrobial activity against various types of Gram-negative; Gram-positive bacteria; and fungi pathogen microorganisms including Escherichia coli , Staphylococcus aureus , and Candida albicans . The cost-effective and eco-friendly biosynthesis of these CuONPs make them particularly attractive in several application from nanotechnology to biomedical science. Article in Journal/Newspaper Antarc* Antarctic Directory of Open Access Journals: DOAJ Articles Antarctic Marine Drugs 19 5 263 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
green synthesis biomaterials metal antibiotics nanotechnology Biology (General) QH301-705.5 |
spellingShingle |
green synthesis biomaterials metal antibiotics nanotechnology Biology (General) QH301-705.5 Maria Sindhura John Joseph Amruthraj Nagoth Marco Zannotti Rita Giovannetti Alessio Mancini Kesava Priyan Ramasamy Cristina Miceli Sandra Pucciarelli Biogenic Synthesis of Copper Nanoparticles Using Bacterial Strains Isolated from an Antarctic Consortium Associated to a Psychrophilic Marine Ciliate: Characterization and Potential Application as Antimicrobial Agents |
topic_facet |
green synthesis biomaterials metal antibiotics nanotechnology Biology (General) QH301-705.5 |
description |
In the last decade, metal nanoparticles (NPs) have gained significant interest in the field of biotechnology due to their unique physiochemical properties and potential uses in a wide range of applications. Metal NP synthesis using microorganisms has emerged as an eco-friendly, clean, and viable strategy alternative to chemical and physical approaches. Herein, an original and efficient route for the microbial synthesis of copper NPs using bacterial strains newly isolated from an Antarctic consortium is described. UV-visible spectra of the NPs showed a maximum absorbance in the range of 380–385 nm. Transmission electron microscopy analysis showed that these NPs are all monodispersed, spherical in nature, and well segregated without any agglomeration and with an average size of 30 nm. X-ray powder diffraction showed a polycrystalline nature and face centered cubic lattice and revealed characteristic diffraction peaks indicating the formation of CuONPs. Fourier-transform infrared spectra confirmed the presence of capping proteins on the NP surface that act as stabilizers. All CuONPs manifested antimicrobial activity against various types of Gram-negative; Gram-positive bacteria; and fungi pathogen microorganisms including Escherichia coli , Staphylococcus aureus , and Candida albicans . The cost-effective and eco-friendly biosynthesis of these CuONPs make them particularly attractive in several application from nanotechnology to biomedical science. |
format |
Article in Journal/Newspaper |
author |
Maria Sindhura John Joseph Amruthraj Nagoth Marco Zannotti Rita Giovannetti Alessio Mancini Kesava Priyan Ramasamy Cristina Miceli Sandra Pucciarelli |
author_facet |
Maria Sindhura John Joseph Amruthraj Nagoth Marco Zannotti Rita Giovannetti Alessio Mancini Kesava Priyan Ramasamy Cristina Miceli Sandra Pucciarelli |
author_sort |
Maria Sindhura John |
title |
Biogenic Synthesis of Copper Nanoparticles Using Bacterial Strains Isolated from an Antarctic Consortium Associated to a Psychrophilic Marine Ciliate: Characterization and Potential Application as Antimicrobial Agents |
title_short |
Biogenic Synthesis of Copper Nanoparticles Using Bacterial Strains Isolated from an Antarctic Consortium Associated to a Psychrophilic Marine Ciliate: Characterization and Potential Application as Antimicrobial Agents |
title_full |
Biogenic Synthesis of Copper Nanoparticles Using Bacterial Strains Isolated from an Antarctic Consortium Associated to a Psychrophilic Marine Ciliate: Characterization and Potential Application as Antimicrobial Agents |
title_fullStr |
Biogenic Synthesis of Copper Nanoparticles Using Bacterial Strains Isolated from an Antarctic Consortium Associated to a Psychrophilic Marine Ciliate: Characterization and Potential Application as Antimicrobial Agents |
title_full_unstemmed |
Biogenic Synthesis of Copper Nanoparticles Using Bacterial Strains Isolated from an Antarctic Consortium Associated to a Psychrophilic Marine Ciliate: Characterization and Potential Application as Antimicrobial Agents |
title_sort |
biogenic synthesis of copper nanoparticles using bacterial strains isolated from an antarctic consortium associated to a psychrophilic marine ciliate: characterization and potential application as antimicrobial agents |
publisher |
MDPI AG |
publishDate |
2021 |
url |
https://doi.org/10.3390/md19050263 https://doaj.org/article/f98f3e18928e4f55854d53787226ff16 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
Marine Drugs, Vol 19, Iss 263, p 263 (2021) |
op_relation |
https://www.mdpi.com/1660-3397/19/5/263 https://doaj.org/toc/1660-3397 doi:10.3390/md19050263 1660-3397 https://doaj.org/article/f98f3e18928e4f55854d53787226ff16 |
op_doi |
https://doi.org/10.3390/md19050263 |
container_title |
Marine Drugs |
container_volume |
19 |
container_issue |
5 |
container_start_page |
263 |
_version_ |
1766271590768574464 |