RNA Interference by Ingested Dsrna-Expressing Bacteria to Study Porphyrin Pigmentation in Crassostrea gigas
Porphyrins are a widespread group of pigments in nature which are believed to contribute to shell colors in mollusks. Previous studies have provided candidate genes for porphyrin shell coloration, however, the linkage analysis between functional genes and porphyrin pigmentation remains unclear in mo...
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ftdoajarticles:oai:doaj.org/article:f98d5f0359bd45ad8f64f21d5ba43627 2023-05-15T15:57:39+02:00 RNA Interference by Ingested Dsrna-Expressing Bacteria to Study Porphyrin Pigmentation in Crassostrea gigas Biyang Hu Qi Li Hong Yu 2021-06-01T00:00:00Z https://doi.org/10.3390/ijms22116120 https://doaj.org/article/f98d5f0359bd45ad8f64f21d5ba43627 EN eng MDPI AG https://www.mdpi.com/1422-0067/22/11/6120 https://doaj.org/toc/1661-6596 https://doaj.org/toc/1422-0067 doi:10.3390/ijms22116120 1422-0067 1661-6596 https://doaj.org/article/f98d5f0359bd45ad8f64f21d5ba43627 International Journal of Molecular Sciences, Vol 22, Iss 6120, p 6120 (2021) shell color pigmentation porphyrin RNA interference Crassostrea gigas Biology (General) QH301-705.5 Chemistry QD1-999 article 2021 ftdoajarticles https://doi.org/10.3390/ijms22116120 2022-12-31T12:26:07Z Porphyrins are a widespread group of pigments in nature which are believed to contribute to shell colors in mollusks. Previous studies have provided candidate genes for porphyrin shell coloration, however, the linkage analysis between functional genes and porphyrin pigmentation remains unclear in mollusks. RNA interference is a powerful molecular tool for analyzing the loss of functions of genes in vivo and alter gene expression. In this study, we used unicellular alga Platymonas subcordiformis and Nitzschia closterium f. minutissima as vectors to feed oysters with Escherichia coli strain HT115 engineered to express double-stranded RNAs targeting specific genes involved in porphyrin synthesis. A strain of Crassostrea gigas with orange shell was used to target key haem pathway genes expression using the aforementioned approach. We show here that feeding the oysters with E. coli , containing dsRNA targeting pigmentation genes, can cause changes in the color of the newly deposited shell. For example, the RNAi knockdown of CgALAS and CgPBGD resulted in the loss of uroporphyrin pigmentation from the shell due to the accumulation of the pigment in the oyster’s mantle. The study probed the crucial role of ALAS and PBGD genes potential functions of uroporphyrin production and shell color pigmentation in C. gigas . Article in Journal/Newspaper Crassostrea gigas Directory of Open Access Journals: DOAJ Articles International Journal of Molecular Sciences 22 11 6120 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
shell color pigmentation porphyrin RNA interference Crassostrea gigas Biology (General) QH301-705.5 Chemistry QD1-999 |
spellingShingle |
shell color pigmentation porphyrin RNA interference Crassostrea gigas Biology (General) QH301-705.5 Chemistry QD1-999 Biyang Hu Qi Li Hong Yu RNA Interference by Ingested Dsrna-Expressing Bacteria to Study Porphyrin Pigmentation in Crassostrea gigas |
topic_facet |
shell color pigmentation porphyrin RNA interference Crassostrea gigas Biology (General) QH301-705.5 Chemistry QD1-999 |
description |
Porphyrins are a widespread group of pigments in nature which are believed to contribute to shell colors in mollusks. Previous studies have provided candidate genes for porphyrin shell coloration, however, the linkage analysis between functional genes and porphyrin pigmentation remains unclear in mollusks. RNA interference is a powerful molecular tool for analyzing the loss of functions of genes in vivo and alter gene expression. In this study, we used unicellular alga Platymonas subcordiformis and Nitzschia closterium f. minutissima as vectors to feed oysters with Escherichia coli strain HT115 engineered to express double-stranded RNAs targeting specific genes involved in porphyrin synthesis. A strain of Crassostrea gigas with orange shell was used to target key haem pathway genes expression using the aforementioned approach. We show here that feeding the oysters with E. coli , containing dsRNA targeting pigmentation genes, can cause changes in the color of the newly deposited shell. For example, the RNAi knockdown of CgALAS and CgPBGD resulted in the loss of uroporphyrin pigmentation from the shell due to the accumulation of the pigment in the oyster’s mantle. The study probed the crucial role of ALAS and PBGD genes potential functions of uroporphyrin production and shell color pigmentation in C. gigas . |
format |
Article in Journal/Newspaper |
author |
Biyang Hu Qi Li Hong Yu |
author_facet |
Biyang Hu Qi Li Hong Yu |
author_sort |
Biyang Hu |
title |
RNA Interference by Ingested Dsrna-Expressing Bacteria to Study Porphyrin Pigmentation in Crassostrea gigas |
title_short |
RNA Interference by Ingested Dsrna-Expressing Bacteria to Study Porphyrin Pigmentation in Crassostrea gigas |
title_full |
RNA Interference by Ingested Dsrna-Expressing Bacteria to Study Porphyrin Pigmentation in Crassostrea gigas |
title_fullStr |
RNA Interference by Ingested Dsrna-Expressing Bacteria to Study Porphyrin Pigmentation in Crassostrea gigas |
title_full_unstemmed |
RNA Interference by Ingested Dsrna-Expressing Bacteria to Study Porphyrin Pigmentation in Crassostrea gigas |
title_sort |
rna interference by ingested dsrna-expressing bacteria to study porphyrin pigmentation in crassostrea gigas |
publisher |
MDPI AG |
publishDate |
2021 |
url |
https://doi.org/10.3390/ijms22116120 https://doaj.org/article/f98d5f0359bd45ad8f64f21d5ba43627 |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
International Journal of Molecular Sciences, Vol 22, Iss 6120, p 6120 (2021) |
op_relation |
https://www.mdpi.com/1422-0067/22/11/6120 https://doaj.org/toc/1661-6596 https://doaj.org/toc/1422-0067 doi:10.3390/ijms22116120 1422-0067 1661-6596 https://doaj.org/article/f98d5f0359bd45ad8f64f21d5ba43627 |
op_doi |
https://doi.org/10.3390/ijms22116120 |
container_title |
International Journal of Molecular Sciences |
container_volume |
22 |
container_issue |
11 |
container_start_page |
6120 |
_version_ |
1766393321589047296 |