Protoplast-mediated transformation of Madurella mycetomatis using hygromycin resistance as a selection marker.
Madurella mycetomatis is the main cause of mycetoma, a chronic granulomatous infection for which currently no adequate therapy is available. To improve therapy, more knowledge on a molecular level is required to understand how M. mycetomatis is able to cause this disease. However, the genetic toolbo...
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ftdoajarticles:oai:doaj.org/article:f947d9254d8c4d988d78b36597ff967c 2024-09-09T19:24:57+00:00 Protoplast-mediated transformation of Madurella mycetomatis using hygromycin resistance as a selection marker. Saskia du Pré Mickey Konings Dorenda J A Schoorl Ahmed H Fahal Mark Arentshorst Arthur F J Ram Wendy W J van de Sande 2024-04-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0012092 https://doaj.org/article/f947d9254d8c4d988d78b36597ff967c EN eng Public Library of Science (PLoS) https://journals.plos.org/plosntds/article/file?id=10.1371/journal.pntd.0012092&type=printable https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0012092 https://doaj.org/article/f947d9254d8c4d988d78b36597ff967c PLoS Neglected Tropical Diseases, Vol 18, Iss 4, p e0012092 (2024) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2024 ftdoajarticles https://doi.org/10.1371/journal.pntd.0012092 2024-08-05T17:49:21Z Madurella mycetomatis is the main cause of mycetoma, a chronic granulomatous infection for which currently no adequate therapy is available. To improve therapy, more knowledge on a molecular level is required to understand how M. mycetomatis is able to cause this disease. However, the genetic toolbox for M. mycetomatis is limited. To date, no method is available to genetically modify M. mycetomatis. In this paper, a protoplast-mediated transformation protocol was successfully developed for this fungal species, using hygromycin as a selection marker. Furthermore, using this method, a cytoplasmic-GFP-expressing M. mycetomatis strain was created. The reported methodology will be invaluable to explore the pathogenicity of M. mycetomatis and to develop reporter strains which can be useful in drug discovery as well as in genetic studies. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 18 4 e0012092 |
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Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Saskia du Pré Mickey Konings Dorenda J A Schoorl Ahmed H Fahal Mark Arentshorst Arthur F J Ram Wendy W J van de Sande Protoplast-mediated transformation of Madurella mycetomatis using hygromycin resistance as a selection marker. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Madurella mycetomatis is the main cause of mycetoma, a chronic granulomatous infection for which currently no adequate therapy is available. To improve therapy, more knowledge on a molecular level is required to understand how M. mycetomatis is able to cause this disease. However, the genetic toolbox for M. mycetomatis is limited. To date, no method is available to genetically modify M. mycetomatis. In this paper, a protoplast-mediated transformation protocol was successfully developed for this fungal species, using hygromycin as a selection marker. Furthermore, using this method, a cytoplasmic-GFP-expressing M. mycetomatis strain was created. The reported methodology will be invaluable to explore the pathogenicity of M. mycetomatis and to develop reporter strains which can be useful in drug discovery as well as in genetic studies. |
format |
Article in Journal/Newspaper |
author |
Saskia du Pré Mickey Konings Dorenda J A Schoorl Ahmed H Fahal Mark Arentshorst Arthur F J Ram Wendy W J van de Sande |
author_facet |
Saskia du Pré Mickey Konings Dorenda J A Schoorl Ahmed H Fahal Mark Arentshorst Arthur F J Ram Wendy W J van de Sande |
author_sort |
Saskia du Pré |
title |
Protoplast-mediated transformation of Madurella mycetomatis using hygromycin resistance as a selection marker. |
title_short |
Protoplast-mediated transformation of Madurella mycetomatis using hygromycin resistance as a selection marker. |
title_full |
Protoplast-mediated transformation of Madurella mycetomatis using hygromycin resistance as a selection marker. |
title_fullStr |
Protoplast-mediated transformation of Madurella mycetomatis using hygromycin resistance as a selection marker. |
title_full_unstemmed |
Protoplast-mediated transformation of Madurella mycetomatis using hygromycin resistance as a selection marker. |
title_sort |
protoplast-mediated transformation of madurella mycetomatis using hygromycin resistance as a selection marker. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2024 |
url |
https://doi.org/10.1371/journal.pntd.0012092 https://doaj.org/article/f947d9254d8c4d988d78b36597ff967c |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 18, Iss 4, p e0012092 (2024) |
op_relation |
https://journals.plos.org/plosntds/article/file?id=10.1371/journal.pntd.0012092&type=printable https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0012092 https://doaj.org/article/f947d9254d8c4d988d78b36597ff967c |
op_doi |
https://doi.org/10.1371/journal.pntd.0012092 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
18 |
container_issue |
4 |
container_start_page |
e0012092 |
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1809894774822404096 |