Production and validation of durable, high quality standardized malaria microscopy slides for teaching, testing and quality assurance during an era of declining diagnostic proficiency
Abstract Background Sets of Giemsa-stained, blood smear slides with systematically verified composite diagnoses would contribute substantially to development of externally validated quality assurance systems for the microscopic diagnosis of malaria. Methods whole blood from Plasmodium -positive dono...
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ftdoajarticles:oai:doaj.org/article:f943fc49acaa42c4950abc1ca51ae5fe 2023-05-15T15:11:05+02:00 Production and validation of durable, high quality standardized malaria microscopy slides for teaching, testing and quality assurance during an era of declining diagnostic proficiency Sismadi Priyanto Muth Sinuon Duong Socheat Jordon Robert G O'Meara Wendy Barcus Mazie J Lederman Edith R Maguire Jason D Bangs Michael J Prescott W Roy Baird J Kevin Wongsrichanalai Chansuda 2006-10-01T00:00:00Z https://doi.org/10.1186/1475-2875-5-92 https://doaj.org/article/f943fc49acaa42c4950abc1ca51ae5fe EN eng BMC http://www.malariajournal.com/content/5/1/92 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-5-92 1475-2875 https://doaj.org/article/f943fc49acaa42c4950abc1ca51ae5fe Malaria Journal, Vol 5, Iss 1, p 92 (2006) Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2006 ftdoajarticles https://doi.org/10.1186/1475-2875-5-92 2022-12-31T08:15:14Z Abstract Background Sets of Giemsa-stained, blood smear slides with systematically verified composite diagnoses would contribute substantially to development of externally validated quality assurance systems for the microscopic diagnosis of malaria. Methods whole blood from Plasmodium -positive donors in Cambodia and Indonesia and individuals with no history of risk for malaria was collected. Using standard operating procedures, technicians prepared Giemsa-stained thick and thin smears from each donor. One slide from each of the first 35 donations was distributed to each of 28 individuals acknowledged by reputation as having expertise in the microscopic diagnosis of malaria. These reference readers recorded presence or absence of Plasmodium species and parasite density. A composite diagnosis for each donation was determined based on microscopic findings and species-specific small subunit ribosomal RNA (ssrRNA) DNA polymerase chain reaction (PCR) amplification. Results More than 12, 000 slides were generated from 124 donations. Reference readers correctly identified presence of parasites on 85% of slides with densities <100 parasites/μl, which improved to 100% for densities >350 parasites/μl. Percentages of agreement with composite diagnoses were highest for Plasmodium falciparum (99%), followed by Plasmodium vivax (86%). Conclusion Herein, a standardized method for producing large numbers of consistently high quality, durable Giemsa-stained blood smears and validating composite diagnoses for the purpose of creating a malaria slide repository in support of initiatives to improve training and competency assessment amidst a background of variability in diagnosis is described. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 5 1 |
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Directory of Open Access Journals: DOAJ Articles |
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English |
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Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
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Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Sismadi Priyanto Muth Sinuon Duong Socheat Jordon Robert G O'Meara Wendy Barcus Mazie J Lederman Edith R Maguire Jason D Bangs Michael J Prescott W Roy Baird J Kevin Wongsrichanalai Chansuda Production and validation of durable, high quality standardized malaria microscopy slides for teaching, testing and quality assurance during an era of declining diagnostic proficiency |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Sets of Giemsa-stained, blood smear slides with systematically verified composite diagnoses would contribute substantially to development of externally validated quality assurance systems for the microscopic diagnosis of malaria. Methods whole blood from Plasmodium -positive donors in Cambodia and Indonesia and individuals with no history of risk for malaria was collected. Using standard operating procedures, technicians prepared Giemsa-stained thick and thin smears from each donor. One slide from each of the first 35 donations was distributed to each of 28 individuals acknowledged by reputation as having expertise in the microscopic diagnosis of malaria. These reference readers recorded presence or absence of Plasmodium species and parasite density. A composite diagnosis for each donation was determined based on microscopic findings and species-specific small subunit ribosomal RNA (ssrRNA) DNA polymerase chain reaction (PCR) amplification. Results More than 12, 000 slides were generated from 124 donations. Reference readers correctly identified presence of parasites on 85% of slides with densities <100 parasites/μl, which improved to 100% for densities >350 parasites/μl. Percentages of agreement with composite diagnoses were highest for Plasmodium falciparum (99%), followed by Plasmodium vivax (86%). Conclusion Herein, a standardized method for producing large numbers of consistently high quality, durable Giemsa-stained blood smears and validating composite diagnoses for the purpose of creating a malaria slide repository in support of initiatives to improve training and competency assessment amidst a background of variability in diagnosis is described. |
format |
Article in Journal/Newspaper |
author |
Sismadi Priyanto Muth Sinuon Duong Socheat Jordon Robert G O'Meara Wendy Barcus Mazie J Lederman Edith R Maguire Jason D Bangs Michael J Prescott W Roy Baird J Kevin Wongsrichanalai Chansuda |
author_facet |
Sismadi Priyanto Muth Sinuon Duong Socheat Jordon Robert G O'Meara Wendy Barcus Mazie J Lederman Edith R Maguire Jason D Bangs Michael J Prescott W Roy Baird J Kevin Wongsrichanalai Chansuda |
author_sort |
Sismadi Priyanto |
title |
Production and validation of durable, high quality standardized malaria microscopy slides for teaching, testing and quality assurance during an era of declining diagnostic proficiency |
title_short |
Production and validation of durable, high quality standardized malaria microscopy slides for teaching, testing and quality assurance during an era of declining diagnostic proficiency |
title_full |
Production and validation of durable, high quality standardized malaria microscopy slides for teaching, testing and quality assurance during an era of declining diagnostic proficiency |
title_fullStr |
Production and validation of durable, high quality standardized malaria microscopy slides for teaching, testing and quality assurance during an era of declining diagnostic proficiency |
title_full_unstemmed |
Production and validation of durable, high quality standardized malaria microscopy slides for teaching, testing and quality assurance during an era of declining diagnostic proficiency |
title_sort |
production and validation of durable, high quality standardized malaria microscopy slides for teaching, testing and quality assurance during an era of declining diagnostic proficiency |
publisher |
BMC |
publishDate |
2006 |
url |
https://doi.org/10.1186/1475-2875-5-92 https://doaj.org/article/f943fc49acaa42c4950abc1ca51ae5fe |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 5, Iss 1, p 92 (2006) |
op_relation |
http://www.malariajournal.com/content/5/1/92 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-5-92 1475-2875 https://doaj.org/article/f943fc49acaa42c4950abc1ca51ae5fe |
op_doi |
https://doi.org/10.1186/1475-2875-5-92 |
container_title |
Malaria Journal |
container_volume |
5 |
container_issue |
1 |
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1766341991851884544 |