A comparison of three approaches for the discovery of novel tripartite attachment complex proteins in Trypanosoma brucei.
Trypanosoma brucei is a single celled eukaryotic parasite and the causative agent of human African trypanosomiasis and nagana in cattle. Aside from its medical relevance, T. brucei has also been key to the discovery of several general biological principles including GPI-anchoring, RNA-editing and tr...
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ftdoajarticles:oai:doaj.org/article:f9347c4a9cd343b88c4d00b44b450a29 2023-05-15T15:04:22+02:00 A comparison of three approaches for the discovery of novel tripartite attachment complex proteins in Trypanosoma brucei. Hélène Clémentine Margareta Baudouin Laura Pfeiffer Torsten Ochsenreiter 2020-09-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0008568 https://doaj.org/article/f9347c4a9cd343b88c4d00b44b450a29 EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0008568 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0008568 https://doaj.org/article/f9347c4a9cd343b88c4d00b44b450a29 PLoS Neglected Tropical Diseases, Vol 14, Iss 9, p e0008568 (2020) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2020 ftdoajarticles https://doi.org/10.1371/journal.pntd.0008568 2022-12-31T07:51:21Z Trypanosoma brucei is a single celled eukaryotic parasite and the causative agent of human African trypanosomiasis and nagana in cattle. Aside from its medical relevance, T. brucei has also been key to the discovery of several general biological principles including GPI-anchoring, RNA-editing and trans-splicing. The parasite contains a single mitochondrion with a singular genome. Recent studies have identified several molecular components of the mitochondrial genome segregation machinery (tripartite attachment complex, TAC), which connects the basal body of the flagellum to the mitochondrial DNA of T. brucei. The TAC component in closest proximity to the mitochondrial DNA is TAC102. Here we apply and compare three different approaches (proximity labelling, immunoprecipitation and yeast two-hybrid) to identify novel interactors of TAC102 and subsequently verify their localisation. Furthermore, we establish the direct interaction of TAC102 and p166 in the unilateral filaments of the TAC. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Tac ENVELOPE(-59.517,-59.517,-62.500,-62.500) PLOS Neglected Tropical Diseases 14 9 e0008568 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Hélène Clémentine Margareta Baudouin Laura Pfeiffer Torsten Ochsenreiter A comparison of three approaches for the discovery of novel tripartite attachment complex proteins in Trypanosoma brucei. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Trypanosoma brucei is a single celled eukaryotic parasite and the causative agent of human African trypanosomiasis and nagana in cattle. Aside from its medical relevance, T. brucei has also been key to the discovery of several general biological principles including GPI-anchoring, RNA-editing and trans-splicing. The parasite contains a single mitochondrion with a singular genome. Recent studies have identified several molecular components of the mitochondrial genome segregation machinery (tripartite attachment complex, TAC), which connects the basal body of the flagellum to the mitochondrial DNA of T. brucei. The TAC component in closest proximity to the mitochondrial DNA is TAC102. Here we apply and compare three different approaches (proximity labelling, immunoprecipitation and yeast two-hybrid) to identify novel interactors of TAC102 and subsequently verify their localisation. Furthermore, we establish the direct interaction of TAC102 and p166 in the unilateral filaments of the TAC. |
format |
Article in Journal/Newspaper |
author |
Hélène Clémentine Margareta Baudouin Laura Pfeiffer Torsten Ochsenreiter |
author_facet |
Hélène Clémentine Margareta Baudouin Laura Pfeiffer Torsten Ochsenreiter |
author_sort |
Hélène Clémentine Margareta Baudouin |
title |
A comparison of three approaches for the discovery of novel tripartite attachment complex proteins in Trypanosoma brucei. |
title_short |
A comparison of three approaches for the discovery of novel tripartite attachment complex proteins in Trypanosoma brucei. |
title_full |
A comparison of three approaches for the discovery of novel tripartite attachment complex proteins in Trypanosoma brucei. |
title_fullStr |
A comparison of three approaches for the discovery of novel tripartite attachment complex proteins in Trypanosoma brucei. |
title_full_unstemmed |
A comparison of three approaches for the discovery of novel tripartite attachment complex proteins in Trypanosoma brucei. |
title_sort |
comparison of three approaches for the discovery of novel tripartite attachment complex proteins in trypanosoma brucei. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2020 |
url |
https://doi.org/10.1371/journal.pntd.0008568 https://doaj.org/article/f9347c4a9cd343b88c4d00b44b450a29 |
long_lat |
ENVELOPE(-59.517,-59.517,-62.500,-62.500) |
geographic |
Arctic Tac |
geographic_facet |
Arctic Tac |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 14, Iss 9, p e0008568 (2020) |
op_relation |
https://doi.org/10.1371/journal.pntd.0008568 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0008568 https://doaj.org/article/f9347c4a9cd343b88c4d00b44b450a29 |
op_doi |
https://doi.org/10.1371/journal.pntd.0008568 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
14 |
container_issue |
9 |
container_start_page |
e0008568 |
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1766336153673269248 |