Succinyl-CoA Synthetase: New Antigen Candidate of Bartonella bacilliformis.
Bartonella bacilliformis is the causative agent of Carrion's disease, a neglected illness with mortality rates of 40-85% in the absence of treatment. The lack of a diagnostic technique to overcome misdiagnosis and treat asymptomatic carriers is of note. This study aimed to identify new B. bacil...
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ftdoajarticles:oai:doaj.org/article:f8d7c9d7013045cf849feb2eb155f8e7 2023-05-15T15:12:03+02:00 Succinyl-CoA Synthetase: New Antigen Candidate of Bartonella bacilliformis. Cláudia Gomes Noemí Palma Maria J Pons Ariel Magallón-Tejada Isabel Sandoval Carmen Tinco-Valdez Carlos Gutarra Juana Del Valle-Mendoza Joaquim Ruiz Mayumi Matsuoka 2016-09-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0004989 https://doaj.org/article/f8d7c9d7013045cf849feb2eb155f8e7 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC5023120?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0004989 https://doaj.org/article/f8d7c9d7013045cf849feb2eb155f8e7 PLoS Neglected Tropical Diseases, Vol 10, Iss 9, p e0004989 (2016) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2016 ftdoajarticles https://doi.org/10.1371/journal.pntd.0004989 2022-12-31T03:53:58Z Bartonella bacilliformis is the causative agent of Carrion's disease, a neglected illness with mortality rates of 40-85% in the absence of treatment. The lack of a diagnostic technique to overcome misdiagnosis and treat asymptomatic carriers is of note. This study aimed to identify new B. bacilliformis antigenic candidates that could lead to a new diagnostic tool able to be implemented in endemic rural areas.Blood (n = 198) and serum (n = 177) samples were collected in northern Peru. Clinical data were recorded. Specific 16S rRNA amplification by RT-PCR, IFA and ELISA for IgM/IgG with whole cells as antigens was done. Western blot analysis and N-terminal amino acid sequencing detected seroreactive proteins. ELISAs for IgM/IgG for the antigenic candidates were performed. Of the population 33.3% reported at least one symptom compatible with Carrion's disease; 25.4% (IFA), 27.1% (ELISA-IgG), 33.9% (ELISA-IgM) and 38.9% (RT-PCR) of samples were positive. Four proteins were considered potential antigenic candidates, including two new antigenic candidates, succinyl-CoA synthetase subunit α (SCS-α) and succinyl-CoA synthetase subunit β (SCS-β). On Western blot both Pap31 and SCS-α interacted with IgM, while GroEL and SCS-β interacted with IgG. The presence of specific antibodies against the antigenic candidates varied from 34.5% (IgG against SCS-α) to 97.2% (IgM against Pap31).RT-PCR and the high levels of positivity for specific ELISAs demonstrate high levels of B. bacilliformis exposure and asymptomatic carriers among inhabitants. The new antigens identified might be used as a new rapid diagnostic tool to diagnose acute Carrion's disease and identify asymptomatic carriers. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 10 9 e0004989 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Cláudia Gomes Noemí Palma Maria J Pons Ariel Magallón-Tejada Isabel Sandoval Carmen Tinco-Valdez Carlos Gutarra Juana Del Valle-Mendoza Joaquim Ruiz Mayumi Matsuoka Succinyl-CoA Synthetase: New Antigen Candidate of Bartonella bacilliformis. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Bartonella bacilliformis is the causative agent of Carrion's disease, a neglected illness with mortality rates of 40-85% in the absence of treatment. The lack of a diagnostic technique to overcome misdiagnosis and treat asymptomatic carriers is of note. This study aimed to identify new B. bacilliformis antigenic candidates that could lead to a new diagnostic tool able to be implemented in endemic rural areas.Blood (n = 198) and serum (n = 177) samples were collected in northern Peru. Clinical data were recorded. Specific 16S rRNA amplification by RT-PCR, IFA and ELISA for IgM/IgG with whole cells as antigens was done. Western blot analysis and N-terminal amino acid sequencing detected seroreactive proteins. ELISAs for IgM/IgG for the antigenic candidates were performed. Of the population 33.3% reported at least one symptom compatible with Carrion's disease; 25.4% (IFA), 27.1% (ELISA-IgG), 33.9% (ELISA-IgM) and 38.9% (RT-PCR) of samples were positive. Four proteins were considered potential antigenic candidates, including two new antigenic candidates, succinyl-CoA synthetase subunit α (SCS-α) and succinyl-CoA synthetase subunit β (SCS-β). On Western blot both Pap31 and SCS-α interacted with IgM, while GroEL and SCS-β interacted with IgG. The presence of specific antibodies against the antigenic candidates varied from 34.5% (IgG against SCS-α) to 97.2% (IgM against Pap31).RT-PCR and the high levels of positivity for specific ELISAs demonstrate high levels of B. bacilliformis exposure and asymptomatic carriers among inhabitants. The new antigens identified might be used as a new rapid diagnostic tool to diagnose acute Carrion's disease and identify asymptomatic carriers. |
format |
Article in Journal/Newspaper |
author |
Cláudia Gomes Noemí Palma Maria J Pons Ariel Magallón-Tejada Isabel Sandoval Carmen Tinco-Valdez Carlos Gutarra Juana Del Valle-Mendoza Joaquim Ruiz Mayumi Matsuoka |
author_facet |
Cláudia Gomes Noemí Palma Maria J Pons Ariel Magallón-Tejada Isabel Sandoval Carmen Tinco-Valdez Carlos Gutarra Juana Del Valle-Mendoza Joaquim Ruiz Mayumi Matsuoka |
author_sort |
Cláudia Gomes |
title |
Succinyl-CoA Synthetase: New Antigen Candidate of Bartonella bacilliformis. |
title_short |
Succinyl-CoA Synthetase: New Antigen Candidate of Bartonella bacilliformis. |
title_full |
Succinyl-CoA Synthetase: New Antigen Candidate of Bartonella bacilliformis. |
title_fullStr |
Succinyl-CoA Synthetase: New Antigen Candidate of Bartonella bacilliformis. |
title_full_unstemmed |
Succinyl-CoA Synthetase: New Antigen Candidate of Bartonella bacilliformis. |
title_sort |
succinyl-coa synthetase: new antigen candidate of bartonella bacilliformis. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2016 |
url |
https://doi.org/10.1371/journal.pntd.0004989 https://doaj.org/article/f8d7c9d7013045cf849feb2eb155f8e7 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 10, Iss 9, p e0004989 (2016) |
op_relation |
http://europepmc.org/articles/PMC5023120?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0004989 https://doaj.org/article/f8d7c9d7013045cf849feb2eb155f8e7 |
op_doi |
https://doi.org/10.1371/journal.pntd.0004989 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
10 |
container_issue |
9 |
container_start_page |
e0004989 |
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1766342795459559424 |