Regulation of gene expression is associated with tolerance of the Arctic copepod Calanus glacialis to CO2‐acidified sea water
Abstract Ocean acidification is the increase in seawater pCO2 due to the uptake of atmospheric anthropogenic CO2, with the largest changes predicted to occur in the Arctic seas. For some marine organisms, this change in pCO2, and associated decrease in pH, represents a climate change‐related stresso...
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ftdoajarticles:oai:doaj.org/article:f847f4f9ac01406c85aa36e8d69d78c3 2023-05-15T14:30:33+02:00 Regulation of gene expression is associated with tolerance of the Arctic copepod Calanus glacialis to CO2‐acidified sea water Allison Bailey Pierre De Wit Peter Thor Howard I. Browman Reidun Bjelland Steven Shema David M. Fields Jeffrey A. Runge Cameron Thompson Haakon Hop 2017-09-01T00:00:00Z https://doi.org/10.1002/ece3.3063 https://doaj.org/article/f847f4f9ac01406c85aa36e8d69d78c3 EN eng Wiley https://doi.org/10.1002/ece3.3063 https://doaj.org/toc/2045-7758 2045-7758 doi:10.1002/ece3.3063 https://doaj.org/article/f847f4f9ac01406c85aa36e8d69d78c3 Ecology and Evolution, Vol 7, Iss 18, Pp 7145-7160 (2017) ocean acidification pH phenotypic buffering RNA‐seq stress response transcriptomics Ecology QH540-549.5 article 2017 ftdoajarticles https://doi.org/10.1002/ece3.3063 2022-12-31T15:32:21Z Abstract Ocean acidification is the increase in seawater pCO2 due to the uptake of atmospheric anthropogenic CO2, with the largest changes predicted to occur in the Arctic seas. For some marine organisms, this change in pCO2, and associated decrease in pH, represents a climate change‐related stressor. In this study, we investigated the gene expression patterns of nauplii of the Arctic copepod Calanus glacialis cultured at low pH levels. We have previously shown that organismal‐level performance (development, growth, respiration) of C. glacialis nauplii is unaffected by low pH. Here, we investigated the molecular‐level response to lowered pH in order to elucidate the physiological processes involved in this tolerance. Nauplii from wild‐caught C. glacialis were cultured at four pH levels (8.05, 7.9, 7.7, 7.5). At stage N6, mRNA was extracted and sequenced using RNA‐seq. The physiological functionality of the proteins identified was categorized using Gene Ontology and KEGG pathways. We found that the expression of 151 contigs varied significantly with pH on a continuous scale (93% downregulated with decreasing pH). Gene set enrichment analysis revealed that, of the processes downregulated, many were components of the universal cellular stress response, including DNA repair, redox regulation, protein folding, and proteolysis. Sodium:proton antiporters were among the processes significantly upregulated, indicating that these ion pumps were involved in maintaining cellular pH homeostasis. C. glacialis significantly alters its gene expression at low pH, although they maintain normal larval development. Understanding what confers tolerance to some species will support our ability to predict the effects of future ocean acidification on marine organisms. Article in Journal/Newspaper Arctic copepod Arctic Calanus glacialis Climate change Ocean acidification Directory of Open Access Journals: DOAJ Articles Arctic Ecology and Evolution 7 18 7145 7160 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
ocean acidification pH phenotypic buffering RNA‐seq stress response transcriptomics Ecology QH540-549.5 |
spellingShingle |
ocean acidification pH phenotypic buffering RNA‐seq stress response transcriptomics Ecology QH540-549.5 Allison Bailey Pierre De Wit Peter Thor Howard I. Browman Reidun Bjelland Steven Shema David M. Fields Jeffrey A. Runge Cameron Thompson Haakon Hop Regulation of gene expression is associated with tolerance of the Arctic copepod Calanus glacialis to CO2‐acidified sea water |
topic_facet |
ocean acidification pH phenotypic buffering RNA‐seq stress response transcriptomics Ecology QH540-549.5 |
description |
Abstract Ocean acidification is the increase in seawater pCO2 due to the uptake of atmospheric anthropogenic CO2, with the largest changes predicted to occur in the Arctic seas. For some marine organisms, this change in pCO2, and associated decrease in pH, represents a climate change‐related stressor. In this study, we investigated the gene expression patterns of nauplii of the Arctic copepod Calanus glacialis cultured at low pH levels. We have previously shown that organismal‐level performance (development, growth, respiration) of C. glacialis nauplii is unaffected by low pH. Here, we investigated the molecular‐level response to lowered pH in order to elucidate the physiological processes involved in this tolerance. Nauplii from wild‐caught C. glacialis were cultured at four pH levels (8.05, 7.9, 7.7, 7.5). At stage N6, mRNA was extracted and sequenced using RNA‐seq. The physiological functionality of the proteins identified was categorized using Gene Ontology and KEGG pathways. We found that the expression of 151 contigs varied significantly with pH on a continuous scale (93% downregulated with decreasing pH). Gene set enrichment analysis revealed that, of the processes downregulated, many were components of the universal cellular stress response, including DNA repair, redox regulation, protein folding, and proteolysis. Sodium:proton antiporters were among the processes significantly upregulated, indicating that these ion pumps were involved in maintaining cellular pH homeostasis. C. glacialis significantly alters its gene expression at low pH, although they maintain normal larval development. Understanding what confers tolerance to some species will support our ability to predict the effects of future ocean acidification on marine organisms. |
format |
Article in Journal/Newspaper |
author |
Allison Bailey Pierre De Wit Peter Thor Howard I. Browman Reidun Bjelland Steven Shema David M. Fields Jeffrey A. Runge Cameron Thompson Haakon Hop |
author_facet |
Allison Bailey Pierre De Wit Peter Thor Howard I. Browman Reidun Bjelland Steven Shema David M. Fields Jeffrey A. Runge Cameron Thompson Haakon Hop |
author_sort |
Allison Bailey |
title |
Regulation of gene expression is associated with tolerance of the Arctic copepod Calanus glacialis to CO2‐acidified sea water |
title_short |
Regulation of gene expression is associated with tolerance of the Arctic copepod Calanus glacialis to CO2‐acidified sea water |
title_full |
Regulation of gene expression is associated with tolerance of the Arctic copepod Calanus glacialis to CO2‐acidified sea water |
title_fullStr |
Regulation of gene expression is associated with tolerance of the Arctic copepod Calanus glacialis to CO2‐acidified sea water |
title_full_unstemmed |
Regulation of gene expression is associated with tolerance of the Arctic copepod Calanus glacialis to CO2‐acidified sea water |
title_sort |
regulation of gene expression is associated with tolerance of the arctic copepod calanus glacialis to co2‐acidified sea water |
publisher |
Wiley |
publishDate |
2017 |
url |
https://doi.org/10.1002/ece3.3063 https://doaj.org/article/f847f4f9ac01406c85aa36e8d69d78c3 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic copepod Arctic Calanus glacialis Climate change Ocean acidification |
genre_facet |
Arctic copepod Arctic Calanus glacialis Climate change Ocean acidification |
op_source |
Ecology and Evolution, Vol 7, Iss 18, Pp 7145-7160 (2017) |
op_relation |
https://doi.org/10.1002/ece3.3063 https://doaj.org/toc/2045-7758 2045-7758 doi:10.1002/ece3.3063 https://doaj.org/article/f847f4f9ac01406c85aa36e8d69d78c3 |
op_doi |
https://doi.org/10.1002/ece3.3063 |
container_title |
Ecology and Evolution |
container_volume |
7 |
container_issue |
18 |
container_start_page |
7145 |
op_container_end_page |
7160 |
_version_ |
1766304384095879168 |