Schistosoma japonicum soluble egg antigens facilitate hepatic stellate cell apoptosis by downregulating Akt expression and upregulating p53 and DR5 expression.
The induction of hepatic stellate cell (HSC) apoptosis has potential as a potent strategy to diminish the progression of liver fibrosis. Previous studies have demonstrated the ability of soluble egg antigens (SEA) from schistosomes to inhibit HSC activation and to induce apoptosis in vitro. In this...
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ftdoajarticles:oai:doaj.org/article:f82bc1ef15634d37981228c65f589557 2023-05-15T15:05:12+02:00 Schistosoma japonicum soluble egg antigens facilitate hepatic stellate cell apoptosis by downregulating Akt expression and upregulating p53 and DR5 expression. Jianxin Wang Feifan Xu Dandan Zhu Yinong Duan Jinling Chen Xiaolei Sun Xue He Pan Li Wei Sun Jinrong Feng 2014-08-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0003106 https://doaj.org/article/f82bc1ef15634d37981228c65f589557 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC4140669?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0003106 https://doaj.org/article/f82bc1ef15634d37981228c65f589557 PLoS Neglected Tropical Diseases, Vol 8, Iss 8, p e3106 (2014) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2014 ftdoajarticles https://doi.org/10.1371/journal.pntd.0003106 2022-12-31T08:44:01Z The induction of hepatic stellate cell (HSC) apoptosis has potential as a potent strategy to diminish the progression of liver fibrosis. Previous studies have demonstrated the ability of soluble egg antigens (SEA) from schistosomes to inhibit HSC activation and to induce apoptosis in vitro. In this study, we aimed to explore the mechanism of SEA-induced apoptosis in HSCs.In this study, we found that SEA could upregulate p53 and DR5 and downregulate the p-Akt. The apoptosis of HSCs induced by SEA could be reduced in HSCs that were treated with p53-specific siRNA and in HSCs that were treated with DR5-specific shRNA. In addition, GW501516, which enhances the expression of Akt, could also decrease the SEA-induced HSC apoptosis. We also found that the increased expression of p53 and DR5 induced by SEA through Mdm2 were reduced by GW501516.Our data suggest that SEA can induce HSC apoptosis by downregulating Akt expression and upregulating p53-dependent DR5 expression. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLoS Neglected Tropical Diseases 8 8 e3106 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
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English |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Jianxin Wang Feifan Xu Dandan Zhu Yinong Duan Jinling Chen Xiaolei Sun Xue He Pan Li Wei Sun Jinrong Feng Schistosoma japonicum soluble egg antigens facilitate hepatic stellate cell apoptosis by downregulating Akt expression and upregulating p53 and DR5 expression. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
The induction of hepatic stellate cell (HSC) apoptosis has potential as a potent strategy to diminish the progression of liver fibrosis. Previous studies have demonstrated the ability of soluble egg antigens (SEA) from schistosomes to inhibit HSC activation and to induce apoptosis in vitro. In this study, we aimed to explore the mechanism of SEA-induced apoptosis in HSCs.In this study, we found that SEA could upregulate p53 and DR5 and downregulate the p-Akt. The apoptosis of HSCs induced by SEA could be reduced in HSCs that were treated with p53-specific siRNA and in HSCs that were treated with DR5-specific shRNA. In addition, GW501516, which enhances the expression of Akt, could also decrease the SEA-induced HSC apoptosis. We also found that the increased expression of p53 and DR5 induced by SEA through Mdm2 were reduced by GW501516.Our data suggest that SEA can induce HSC apoptosis by downregulating Akt expression and upregulating p53-dependent DR5 expression. |
format |
Article in Journal/Newspaper |
author |
Jianxin Wang Feifan Xu Dandan Zhu Yinong Duan Jinling Chen Xiaolei Sun Xue He Pan Li Wei Sun Jinrong Feng |
author_facet |
Jianxin Wang Feifan Xu Dandan Zhu Yinong Duan Jinling Chen Xiaolei Sun Xue He Pan Li Wei Sun Jinrong Feng |
author_sort |
Jianxin Wang |
title |
Schistosoma japonicum soluble egg antigens facilitate hepatic stellate cell apoptosis by downregulating Akt expression and upregulating p53 and DR5 expression. |
title_short |
Schistosoma japonicum soluble egg antigens facilitate hepatic stellate cell apoptosis by downregulating Akt expression and upregulating p53 and DR5 expression. |
title_full |
Schistosoma japonicum soluble egg antigens facilitate hepatic stellate cell apoptosis by downregulating Akt expression and upregulating p53 and DR5 expression. |
title_fullStr |
Schistosoma japonicum soluble egg antigens facilitate hepatic stellate cell apoptosis by downregulating Akt expression and upregulating p53 and DR5 expression. |
title_full_unstemmed |
Schistosoma japonicum soluble egg antigens facilitate hepatic stellate cell apoptosis by downregulating Akt expression and upregulating p53 and DR5 expression. |
title_sort |
schistosoma japonicum soluble egg antigens facilitate hepatic stellate cell apoptosis by downregulating akt expression and upregulating p53 and dr5 expression. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2014 |
url |
https://doi.org/10.1371/journal.pntd.0003106 https://doaj.org/article/f82bc1ef15634d37981228c65f589557 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 8, Iss 8, p e3106 (2014) |
op_relation |
http://europepmc.org/articles/PMC4140669?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0003106 https://doaj.org/article/f82bc1ef15634d37981228c65f589557 |
op_doi |
https://doi.org/10.1371/journal.pntd.0003106 |
container_title |
PLoS Neglected Tropical Diseases |
container_volume |
8 |
container_issue |
8 |
container_start_page |
e3106 |
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1766336944653991936 |