Biochemical comparison of venoms from young Colombian Crotalus durissus cumanensis and their parents
Crotalus durissus cumanensis, a rattlesnake endemic to Colombia and Venezuela, is considered one of the most lethal snake species in Latin America. The aim of the present study was to compare the protein content and biological activity of the venom obtained from eight specimens of C. durissus cumane...
Published in: | Journal of Venomous Animals and Toxins including Tropical Diseases |
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ftdoajarticles:oai:doaj.org/article:f7f04483d4f04f25a5a1176068301c0a 2023-05-15T15:06:54+02:00 Biochemical comparison of venoms from young Colombian Crotalus durissus cumanensis and their parents N Céspedes F Castro E Jiménez L Montealegre A Castellanos CA Cañas M Arévalo-Herrera S Herrera 2010-01-01T00:00:00Z https://doi.org/10.1590/S1678-91992010000200009 https://doaj.org/article/f7f04483d4f04f25a5a1176068301c0a EN eng SciELO http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000200009 https://doaj.org/toc/1678-9199 doi:10.1590/S1678-91992010000200009 1678-9199 https://doaj.org/article/f7f04483d4f04f25a5a1176068301c0a Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 16, Iss 2, Pp 268-284 (2010) snakes Crotalus durissus cumanensis rattlesnake venom Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 article 2010 ftdoajarticles https://doi.org/10.1590/S1678-91992010000200009 2022-12-31T00:09:45Z Crotalus durissus cumanensis, a rattlesnake endemic to Colombia and Venezuela, is considered one of the most lethal snake species in Latin America. The aim of the present study was to compare the protein content and biological activity of the venom obtained from eight specimens of C. durissus cumanensis, namely two adults from different localities of Colombia and six offspring born in captivity. Protein profiles of crude venoms were analyzed by SDS-PAGE and RP-HPLC, and biological activities were evaluated for lethality, edema, defibrination, hemolytic and coagulant activities to assess individual venoms of adults and a pool of young snake venoms. Transient edema appeared rapidly after venom inoculation, whereas hemorrhagic effect was not observed. Differences in protein profiles, lethality, hemolytic, coagulant and defibrinating activities between both adult snake venoms were observed; those from the mother snake exhibited higher activities. Venoms from young snakes were similar to the one obtained from the mother, but the coagulant effect was stronger in offspring venoms. Notably, biological effects of the father snake venom were not comparable to those previously described for C. durissus cumanensis from Venezuela and C. durissus terrificus from Brazil, confirming the high variability of the venom from Crotalus species. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Journal of Venomous Animals and Toxins including Tropical Diseases 16 2 268 284 |
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Directory of Open Access Journals: DOAJ Articles |
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language |
English |
topic |
snakes Crotalus durissus cumanensis rattlesnake venom Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
spellingShingle |
snakes Crotalus durissus cumanensis rattlesnake venom Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 N Céspedes F Castro E Jiménez L Montealegre A Castellanos CA Cañas M Arévalo-Herrera S Herrera Biochemical comparison of venoms from young Colombian Crotalus durissus cumanensis and their parents |
topic_facet |
snakes Crotalus durissus cumanensis rattlesnake venom Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
description |
Crotalus durissus cumanensis, a rattlesnake endemic to Colombia and Venezuela, is considered one of the most lethal snake species in Latin America. The aim of the present study was to compare the protein content and biological activity of the venom obtained from eight specimens of C. durissus cumanensis, namely two adults from different localities of Colombia and six offspring born in captivity. Protein profiles of crude venoms were analyzed by SDS-PAGE and RP-HPLC, and biological activities were evaluated for lethality, edema, defibrination, hemolytic and coagulant activities to assess individual venoms of adults and a pool of young snake venoms. Transient edema appeared rapidly after venom inoculation, whereas hemorrhagic effect was not observed. Differences in protein profiles, lethality, hemolytic, coagulant and defibrinating activities between both adult snake venoms were observed; those from the mother snake exhibited higher activities. Venoms from young snakes were similar to the one obtained from the mother, but the coagulant effect was stronger in offspring venoms. Notably, biological effects of the father snake venom were not comparable to those previously described for C. durissus cumanensis from Venezuela and C. durissus terrificus from Brazil, confirming the high variability of the venom from Crotalus species. |
format |
Article in Journal/Newspaper |
author |
N Céspedes F Castro E Jiménez L Montealegre A Castellanos CA Cañas M Arévalo-Herrera S Herrera |
author_facet |
N Céspedes F Castro E Jiménez L Montealegre A Castellanos CA Cañas M Arévalo-Herrera S Herrera |
author_sort |
N Céspedes |
title |
Biochemical comparison of venoms from young Colombian Crotalus durissus cumanensis and their parents |
title_short |
Biochemical comparison of venoms from young Colombian Crotalus durissus cumanensis and their parents |
title_full |
Biochemical comparison of venoms from young Colombian Crotalus durissus cumanensis and their parents |
title_fullStr |
Biochemical comparison of venoms from young Colombian Crotalus durissus cumanensis and their parents |
title_full_unstemmed |
Biochemical comparison of venoms from young Colombian Crotalus durissus cumanensis and their parents |
title_sort |
biochemical comparison of venoms from young colombian crotalus durissus cumanensis and their parents |
publisher |
SciELO |
publishDate |
2010 |
url |
https://doi.org/10.1590/S1678-91992010000200009 https://doaj.org/article/f7f04483d4f04f25a5a1176068301c0a |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 16, Iss 2, Pp 268-284 (2010) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000200009 https://doaj.org/toc/1678-9199 doi:10.1590/S1678-91992010000200009 1678-9199 https://doaj.org/article/f7f04483d4f04f25a5a1176068301c0a |
op_doi |
https://doi.org/10.1590/S1678-91992010000200009 |
container_title |
Journal of Venomous Animals and Toxins including Tropical Diseases |
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16 |
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2 |
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268 |
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284 |
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