Cultivable marine fungi from the Arctic Archipelago of Svalbard and their antibacterial activity
During a research cruise in 2016, we isolated fungi from sediments, seawater, driftwood, fruiting bodies, and macroalgae using three different media to assess species richness and potential bioactivity of cultivable marine fungi in the High Arctic region. Ten stations from the Svalbard archipelago (...
Published in: | Mycology |
---|---|
Main Authors: | , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Taylor & Francis Group
2020
|
Subjects: | |
Online Access: | https://doi.org/10.1080/21501203.2019.1708492 https://doaj.org/article/f7e055b08dd943d3ad4d8780dc632380 |
id |
ftdoajarticles:oai:doaj.org/article:f7e055b08dd943d3ad4d8780dc632380 |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:f7e055b08dd943d3ad4d8780dc632380 2023-05-15T14:28:53+02:00 Cultivable marine fungi from the Arctic Archipelago of Svalbard and their antibacterial activity Ole Christian Hagestad Jeanette H. Andersen Bjørn Altermark Espen Hansen Teppo Rämä 2020-07-01T00:00:00Z https://doi.org/10.1080/21501203.2019.1708492 https://doaj.org/article/f7e055b08dd943d3ad4d8780dc632380 EN eng Taylor & Francis Group http://dx.doi.org/10.1080/21501203.2019.1708492 https://doaj.org/toc/2150-1203 https://doaj.org/toc/2150-1211 2150-1203 2150-1211 doi:10.1080/21501203.2019.1708492 https://doaj.org/article/f7e055b08dd943d3ad4d8780dc632380 Mycology, Vol 11, Iss 3, Pp 230-242 (2020) agar plug diffusion assay barents sea bioactivity dna barcoding lulworthiales molecular phylogeny Biology (General) QH301-705.5 Microbiology QR1-502 article 2020 ftdoajarticles https://doi.org/10.1080/21501203.2019.1708492 2022-12-31T05:10:58Z During a research cruise in 2016, we isolated fungi from sediments, seawater, driftwood, fruiting bodies, and macroalgae using three different media to assess species richness and potential bioactivity of cultivable marine fungi in the High Arctic region. Ten stations from the Svalbard archipelago (73–80 °N, 18–31 °E) were investigated and 33 fungal isolates were obtained. These grouped into 22 operational taxonomic units (OTUs) using nuc rDNA internal transcribed spacer regions (ITS1-5.8S-ITS2 = ITS) with acut-off set at 98% similarity. The taxonomic analysis showed that 17 OTUs belonged to Ascomycota, one to Basidiomycota, two to Mucoromycota and two were fungal-like organisms. The nuc rDNA V1-V5 regions of 18S (18S) and D1-D3 regions of 28S (28S) were sequenced from representative isolates of each OTU for comparison to GenBank sequences. Isolates of Lulworthiales and Eurotiales were the most abundant, with seven isolates each. Among the 22 OTUs, nine represent potentially undescribed species based on low similarity to GenBank sequences and 10 isolates showed inhibitory activity against Gram-positive bacteria in an agar diffusion plug assay. These results show promise for the Arctic region as asource of novel marine fungi with the ability to produce bioactive secondary metabolites with antibacterial properties. Article in Journal/Newspaper Arctic Archipelago Arctic Barents Sea Svalbard Directory of Open Access Journals: DOAJ Articles Arctic Svalbard Barents Sea Svalbard Archipelago Mycology 11 3 230 242 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
agar plug diffusion assay barents sea bioactivity dna barcoding lulworthiales molecular phylogeny Biology (General) QH301-705.5 Microbiology QR1-502 |
spellingShingle |
agar plug diffusion assay barents sea bioactivity dna barcoding lulworthiales molecular phylogeny Biology (General) QH301-705.5 Microbiology QR1-502 Ole Christian Hagestad Jeanette H. Andersen Bjørn Altermark Espen Hansen Teppo Rämä Cultivable marine fungi from the Arctic Archipelago of Svalbard and their antibacterial activity |
topic_facet |
agar plug diffusion assay barents sea bioactivity dna barcoding lulworthiales molecular phylogeny Biology (General) QH301-705.5 Microbiology QR1-502 |
description |
During a research cruise in 2016, we isolated fungi from sediments, seawater, driftwood, fruiting bodies, and macroalgae using three different media to assess species richness and potential bioactivity of cultivable marine fungi in the High Arctic region. Ten stations from the Svalbard archipelago (73–80 °N, 18–31 °E) were investigated and 33 fungal isolates were obtained. These grouped into 22 operational taxonomic units (OTUs) using nuc rDNA internal transcribed spacer regions (ITS1-5.8S-ITS2 = ITS) with acut-off set at 98% similarity. The taxonomic analysis showed that 17 OTUs belonged to Ascomycota, one to Basidiomycota, two to Mucoromycota and two were fungal-like organisms. The nuc rDNA V1-V5 regions of 18S (18S) and D1-D3 regions of 28S (28S) were sequenced from representative isolates of each OTU for comparison to GenBank sequences. Isolates of Lulworthiales and Eurotiales were the most abundant, with seven isolates each. Among the 22 OTUs, nine represent potentially undescribed species based on low similarity to GenBank sequences and 10 isolates showed inhibitory activity against Gram-positive bacteria in an agar diffusion plug assay. These results show promise for the Arctic region as asource of novel marine fungi with the ability to produce bioactive secondary metabolites with antibacterial properties. |
format |
Article in Journal/Newspaper |
author |
Ole Christian Hagestad Jeanette H. Andersen Bjørn Altermark Espen Hansen Teppo Rämä |
author_facet |
Ole Christian Hagestad Jeanette H. Andersen Bjørn Altermark Espen Hansen Teppo Rämä |
author_sort |
Ole Christian Hagestad |
title |
Cultivable marine fungi from the Arctic Archipelago of Svalbard and their antibacterial activity |
title_short |
Cultivable marine fungi from the Arctic Archipelago of Svalbard and their antibacterial activity |
title_full |
Cultivable marine fungi from the Arctic Archipelago of Svalbard and their antibacterial activity |
title_fullStr |
Cultivable marine fungi from the Arctic Archipelago of Svalbard and their antibacterial activity |
title_full_unstemmed |
Cultivable marine fungi from the Arctic Archipelago of Svalbard and their antibacterial activity |
title_sort |
cultivable marine fungi from the arctic archipelago of svalbard and their antibacterial activity |
publisher |
Taylor & Francis Group |
publishDate |
2020 |
url |
https://doi.org/10.1080/21501203.2019.1708492 https://doaj.org/article/f7e055b08dd943d3ad4d8780dc632380 |
geographic |
Arctic Svalbard Barents Sea Svalbard Archipelago |
geographic_facet |
Arctic Svalbard Barents Sea Svalbard Archipelago |
genre |
Arctic Archipelago Arctic Barents Sea Svalbard |
genre_facet |
Arctic Archipelago Arctic Barents Sea Svalbard |
op_source |
Mycology, Vol 11, Iss 3, Pp 230-242 (2020) |
op_relation |
http://dx.doi.org/10.1080/21501203.2019.1708492 https://doaj.org/toc/2150-1203 https://doaj.org/toc/2150-1211 2150-1203 2150-1211 doi:10.1080/21501203.2019.1708492 https://doaj.org/article/f7e055b08dd943d3ad4d8780dc632380 |
op_doi |
https://doi.org/10.1080/21501203.2019.1708492 |
container_title |
Mycology |
container_volume |
11 |
container_issue |
3 |
container_start_page |
230 |
op_container_end_page |
242 |
_version_ |
1766303025999118336 |