International external quality assessment of molecular detection of Rift Valley fever virus.
Rift Valley fever (RVF) is a viral zoonosis that primarily affects animals resulting in considerable economic losses due to death and abortions among infected livestock. RVF also affects humans with clinical symptoms ranging from an influenza-like illness to a hemorrhagic fever. Over the past years,...
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ftdoajarticles:oai:doaj.org/article:f7c9a0fedbc2458693465e617a78eaab 2023-05-15T15:13:36+02:00 International external quality assessment of molecular detection of Rift Valley fever virus. Camille Escadafal Janusz T Paweska Antoinette Grobbelaar Chantel le Roux Michèle Bouloy Pranav Patel Anette Teichmann Oliver Donoso-Mantke Matthias Niedrig 2013-01-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0002244 https://doaj.org/article/f7c9a0fedbc2458693465e617a78eaab EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC3662703?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0002244 https://doaj.org/article/f7c9a0fedbc2458693465e617a78eaab PLoS Neglected Tropical Diseases, Vol 7, Iss 5, p e2244 (2013) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2013 ftdoajarticles https://doi.org/10.1371/journal.pntd.0002244 2022-12-31T08:41:49Z Rift Valley fever (RVF) is a viral zoonosis that primarily affects animals resulting in considerable economic losses due to death and abortions among infected livestock. RVF also affects humans with clinical symptoms ranging from an influenza-like illness to a hemorrhagic fever. Over the past years, RVF virus (RVFV) has caused severe outbreaks in livestock and humans throughout Africa and regions of the world previously regarded as free of the virus. This situation prompts the need to evaluate the diagnostic capacity and performance of laboratories worldwide. Diagnostic methods for RVFV detection include virus isolation, antigen and antibody detection methods, and nucleic acid amplification techniques. Molecular methods such as reverse-transcriptase polymerase chain reaction and other newly developed techniques allow for a rapid and accurate detection of RVFV. This study aims to assess the efficiency and accurateness of RVFV molecular diagnostic methods used by expert laboratories worldwide. Thirty expert laboratories from 16 countries received a panel of 14 samples which included RVFV preparations representing several genetic lineages, a specificity control and negative controls. In this study we present the results of the first international external quality assessment (EQA) for the molecular diagnosis of RVF. Optimal results were reported by 64% of the analyses, 21% of the analyses achieved acceptable results and 15% of the results revealed that there is need for improvement. Evenly good performances were achieved by specific protocols which can therefore be recommended as an accurate molecular protocol for the diagnosis of RVF. Other protocols showed uneven performances revealing the need for improved optimization and standardization of these protocols. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLoS Neglected Tropical Diseases 7 5 e2244 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Camille Escadafal Janusz T Paweska Antoinette Grobbelaar Chantel le Roux Michèle Bouloy Pranav Patel Anette Teichmann Oliver Donoso-Mantke Matthias Niedrig International external quality assessment of molecular detection of Rift Valley fever virus. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Rift Valley fever (RVF) is a viral zoonosis that primarily affects animals resulting in considerable economic losses due to death and abortions among infected livestock. RVF also affects humans with clinical symptoms ranging from an influenza-like illness to a hemorrhagic fever. Over the past years, RVF virus (RVFV) has caused severe outbreaks in livestock and humans throughout Africa and regions of the world previously regarded as free of the virus. This situation prompts the need to evaluate the diagnostic capacity and performance of laboratories worldwide. Diagnostic methods for RVFV detection include virus isolation, antigen and antibody detection methods, and nucleic acid amplification techniques. Molecular methods such as reverse-transcriptase polymerase chain reaction and other newly developed techniques allow for a rapid and accurate detection of RVFV. This study aims to assess the efficiency and accurateness of RVFV molecular diagnostic methods used by expert laboratories worldwide. Thirty expert laboratories from 16 countries received a panel of 14 samples which included RVFV preparations representing several genetic lineages, a specificity control and negative controls. In this study we present the results of the first international external quality assessment (EQA) for the molecular diagnosis of RVF. Optimal results were reported by 64% of the analyses, 21% of the analyses achieved acceptable results and 15% of the results revealed that there is need for improvement. Evenly good performances were achieved by specific protocols which can therefore be recommended as an accurate molecular protocol for the diagnosis of RVF. Other protocols showed uneven performances revealing the need for improved optimization and standardization of these protocols. |
format |
Article in Journal/Newspaper |
author |
Camille Escadafal Janusz T Paweska Antoinette Grobbelaar Chantel le Roux Michèle Bouloy Pranav Patel Anette Teichmann Oliver Donoso-Mantke Matthias Niedrig |
author_facet |
Camille Escadafal Janusz T Paweska Antoinette Grobbelaar Chantel le Roux Michèle Bouloy Pranav Patel Anette Teichmann Oliver Donoso-Mantke Matthias Niedrig |
author_sort |
Camille Escadafal |
title |
International external quality assessment of molecular detection of Rift Valley fever virus. |
title_short |
International external quality assessment of molecular detection of Rift Valley fever virus. |
title_full |
International external quality assessment of molecular detection of Rift Valley fever virus. |
title_fullStr |
International external quality assessment of molecular detection of Rift Valley fever virus. |
title_full_unstemmed |
International external quality assessment of molecular detection of Rift Valley fever virus. |
title_sort |
international external quality assessment of molecular detection of rift valley fever virus. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2013 |
url |
https://doi.org/10.1371/journal.pntd.0002244 https://doaj.org/article/f7c9a0fedbc2458693465e617a78eaab |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 7, Iss 5, p e2244 (2013) |
op_relation |
http://europepmc.org/articles/PMC3662703?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0002244 https://doaj.org/article/f7c9a0fedbc2458693465e617a78eaab |
op_doi |
https://doi.org/10.1371/journal.pntd.0002244 |
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PLoS Neglected Tropical Diseases |
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7 |
container_issue |
5 |
container_start_page |
e2244 |
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