var gene transcription and PfEMP1 expression in the rosetting and cytoadhesive Plasmodium falciparum clone FCR3S1.2
Abstract Background The pathogenicity of Plasmodium falciparum is in part due to the ability of the parasitized red blood cell (pRBC) to adhere to intra-vascular host cell receptors and serum-proteins. Binding of the pRBC is mediated by Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1),...
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ftdoajarticles:oai:doaj.org/article:f7c325e2b07340e7a98d0274d8badaa0 2023-05-15T15:12:27+02:00 var gene transcription and PfEMP1 expression in the rosetting and cytoadhesive Plasmodium falciparum clone FCR3S1.2 Chen Qijun Normark Johan Blomqvist Karin Moll Kirsten Albrecht Letusa Wahlgren Mats 2011-01-01T00:00:00Z https://doi.org/10.1186/1475-2875-10-17 https://doaj.org/article/f7c325e2b07340e7a98d0274d8badaa0 EN eng BMC http://www.malariajournal.com/content/10/1/17 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-10-17 1475-2875 https://doaj.org/article/f7c325e2b07340e7a98d0274d8badaa0 Malaria Journal, Vol 10, Iss 1, p 17 (2011) Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2011 ftdoajarticles https://doi.org/10.1186/1475-2875-10-17 2022-12-31T02:57:47Z Abstract Background The pathogenicity of Plasmodium falciparum is in part due to the ability of the parasitized red blood cell (pRBC) to adhere to intra-vascular host cell receptors and serum-proteins. Binding of the pRBC is mediated by Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1), a large multi-variant molecule encoded by a family of ≈60 var genes. Methods The study of var gene transcription in the parasite clone FCR3S1.2 was performed by semi-quantitative PCR and quantitative PCR (qPCR). The expression of the major PfEMP1 in FCR3S1.2 pRBC was analysed with polyclonal sera in rosette disruption assays and immunofluorecence. Results Transcripts from var 1 (FCR3S1.2 var 1 IT4 var 21) and other var genes were detected by semi-quantitative PCR but results from qPCR showed that one var gene transcript dominated over the others (FCR3S1.2 var 2 IT4 var 60). Antibodies raised in rats to the recombinant NTS-DBL1α of var 2 produced in E. coli completely and dose-dependently disrupted rosettes (≈95% at a dilution of 1/5). The sera reacted with the Maurer's clefts in trophozoite stages (IFA) and to the infected erythrocyte surface (FACS) indicating that FCR3S1.2 var2 encodes the dominant PfEMP1 expressed in this parasite. Conclusion The major transcript in the rosetting model parasite FCR3S1.2 is FCR3S1.2 var 2 (IT4 var 60). The results suggest that this gene encodes the PfEMP1-species responsible for the rosetting phenotype of this parasite. The activity of previously raised antibodies to the NTS-DBL1α of FCR3S1.2 var 1 is likely due to cross-reactivity with NTS-DBL1α of the var 2 encoded PfEMP1. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 10 1 17 |
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Directory of Open Access Journals: DOAJ Articles |
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English |
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Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
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Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Chen Qijun Normark Johan Blomqvist Karin Moll Kirsten Albrecht Letusa Wahlgren Mats var gene transcription and PfEMP1 expression in the rosetting and cytoadhesive Plasmodium falciparum clone FCR3S1.2 |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background The pathogenicity of Plasmodium falciparum is in part due to the ability of the parasitized red blood cell (pRBC) to adhere to intra-vascular host cell receptors and serum-proteins. Binding of the pRBC is mediated by Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1), a large multi-variant molecule encoded by a family of ≈60 var genes. Methods The study of var gene transcription in the parasite clone FCR3S1.2 was performed by semi-quantitative PCR and quantitative PCR (qPCR). The expression of the major PfEMP1 in FCR3S1.2 pRBC was analysed with polyclonal sera in rosette disruption assays and immunofluorecence. Results Transcripts from var 1 (FCR3S1.2 var 1 IT4 var 21) and other var genes were detected by semi-quantitative PCR but results from qPCR showed that one var gene transcript dominated over the others (FCR3S1.2 var 2 IT4 var 60). Antibodies raised in rats to the recombinant NTS-DBL1α of var 2 produced in E. coli completely and dose-dependently disrupted rosettes (≈95% at a dilution of 1/5). The sera reacted with the Maurer's clefts in trophozoite stages (IFA) and to the infected erythrocyte surface (FACS) indicating that FCR3S1.2 var2 encodes the dominant PfEMP1 expressed in this parasite. Conclusion The major transcript in the rosetting model parasite FCR3S1.2 is FCR3S1.2 var 2 (IT4 var 60). The results suggest that this gene encodes the PfEMP1-species responsible for the rosetting phenotype of this parasite. The activity of previously raised antibodies to the NTS-DBL1α of FCR3S1.2 var 1 is likely due to cross-reactivity with NTS-DBL1α of the var 2 encoded PfEMP1. |
format |
Article in Journal/Newspaper |
author |
Chen Qijun Normark Johan Blomqvist Karin Moll Kirsten Albrecht Letusa Wahlgren Mats |
author_facet |
Chen Qijun Normark Johan Blomqvist Karin Moll Kirsten Albrecht Letusa Wahlgren Mats |
author_sort |
Chen Qijun |
title |
var gene transcription and PfEMP1 expression in the rosetting and cytoadhesive Plasmodium falciparum clone FCR3S1.2 |
title_short |
var gene transcription and PfEMP1 expression in the rosetting and cytoadhesive Plasmodium falciparum clone FCR3S1.2 |
title_full |
var gene transcription and PfEMP1 expression in the rosetting and cytoadhesive Plasmodium falciparum clone FCR3S1.2 |
title_fullStr |
var gene transcription and PfEMP1 expression in the rosetting and cytoadhesive Plasmodium falciparum clone FCR3S1.2 |
title_full_unstemmed |
var gene transcription and PfEMP1 expression in the rosetting and cytoadhesive Plasmodium falciparum clone FCR3S1.2 |
title_sort |
var gene transcription and pfemp1 expression in the rosetting and cytoadhesive plasmodium falciparum clone fcr3s1.2 |
publisher |
BMC |
publishDate |
2011 |
url |
https://doi.org/10.1186/1475-2875-10-17 https://doaj.org/article/f7c325e2b07340e7a98d0274d8badaa0 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 10, Iss 1, p 17 (2011) |
op_relation |
http://www.malariajournal.com/content/10/1/17 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-10-17 1475-2875 https://doaj.org/article/f7c325e2b07340e7a98d0274d8badaa0 |
op_doi |
https://doi.org/10.1186/1475-2875-10-17 |
container_title |
Malaria Journal |
container_volume |
10 |
container_issue |
1 |
container_start_page |
17 |
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1766343121419894784 |