Solvent-dependent activity of Candida antarctica lipase B and its correlation with a regioselective mono aza-Michael addition - experimental and molecular dynamics simulation studies

• Published in: Heliyon
• Main Authors: Zohreh Nazarian, Seyed Shahriar Arab
• Format: Article in Journal/Newspaper
• Language: English
• Published: Elsevier 2022
• Subjects: aza-Michael addition; Regioselective synthesis; CALB; Biocatalysis; Computational simulation; Molecular dynamics simulation; Science (General); Q1-390; Social sciences (General); H1-99; Antarc*; Antarctica
• Online Access: https://doi.org/10.1016/j.heliyon.2022.e10336; https://doaj.org/article/f7721dbf7a544b14a82e47d2bb883bd1

With the aim of gaining understanding of the molecular basis of commercially available Candida antarctica lipase B (CALB) immobilized on polyacrylic resin catalyzed regioselective mono aza-Michael addition of Benzhydrazide to Diethyl maleate we decided to carry out molecular dynamics (MD) simulation studies in parallel with our experimental study. We found a correlation between the activity of CALB and the choice of solvent. Our study showed that solvent affects the performance of the enzyme due to the binding of solvent molecules to the enzyme active site region, and the solvation energy of substrates in the different solvents. We also found that CALB is only active in nonpolar solvent (i.e. Hexane), and therefore we investigated the influence of Hexane on the catalytic activity of CALB for the reaction. The results of this study and related experimental validation from our studies have been discussed here.