Evaluation of Polymeric Matrix Loaded with Melatonin for Wound Dressing
The development of scaffolds mimicking the extracellular matrix containing bioactive substances has great potential in tissue engineering and wound healing applications. This study investigates melatonin—a methoxyindole present in almost all biological systems. Melatonin is a bioregulator in terms o...
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ftdoajarticles:oai:doaj.org/article:f7716c1cc60043a889e10f216620025f 2023-05-15T18:09:53+02:00 Evaluation of Polymeric Matrix Loaded with Melatonin for Wound Dressing Beata Kaczmarek-Szczepańska Justyna Ostrowska Justyna Kozłowska Zofia Szota Anna A. Brożyna Rita Dreier Russel J. Reiter Andrzej T. Slominski Kerstin Steinbrink Konrad Kleszczyński 2021-05-01T00:00:00Z https://doi.org/10.3390/ijms22115658 https://doaj.org/article/f7716c1cc60043a889e10f216620025f EN eng MDPI AG https://www.mdpi.com/1422-0067/22/11/5658 https://doaj.org/toc/1661-6596 https://doaj.org/toc/1422-0067 doi:10.3390/ijms22115658 1422-0067 1661-6596 https://doaj.org/article/f7716c1cc60043a889e10f216620025f International Journal of Molecular Sciences, Vol 22, Iss 5658, p 5658 (2021) melatonin scaffolds biopolymers collagen chitosan cutaneous cells Biology (General) QH301-705.5 Chemistry QD1-999 article 2021 ftdoajarticles https://doi.org/10.3390/ijms22115658 2022-12-31T12:26:12Z The development of scaffolds mimicking the extracellular matrix containing bioactive substances has great potential in tissue engineering and wound healing applications. This study investigates melatonin—a methoxyindole present in almost all biological systems. Melatonin is a bioregulator in terms of its potential clinical importance for future therapies of cutaneous diseases. Mammalian skin is not only a prominent melatonin target, but also produces and rapidly metabolizes the multifunctional methoxyindole to biologically active metabolites. In our methodology, chitosan/collagen (CTS/Coll)-contained biomaterials are blended with melatonin at different doses to fabricate biomimetic hybrid scaffolds. We use rat tail tendon- and Salmo salar fish skin-derived collagens to assess biophysical and cellular properties by ( i ) Fourier transform infrared spectroscopy—attenuated total reflectance (FTIR–ATR), ( ii ) thermogravimetric analysis (TG), ( iii ) scanning electron microscope (SEM), and ( iv ) proliferation ratio of cutaneous cells in vitro. Our results indicate that melatonin itself does not negatively affect biophysical properties of melatonin-immobilized hybrid scaffolds, but it induces a pronounced elevation of cell viability within human epidermal keratinocytes (NHEK), dermal fibroblasts (NHDF), and reference melanoma cells. These results demonstrate that this indoleamine accelerates re-epithelialization. This delivery is a promising technique for additional explorations in future dermatotherapy and protective skin medicine. Article in Journal/Newspaper Salmo salar Directory of Open Access Journals: DOAJ Articles International Journal of Molecular Sciences 22 11 5658 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
melatonin scaffolds biopolymers collagen chitosan cutaneous cells Biology (General) QH301-705.5 Chemistry QD1-999 |
spellingShingle |
melatonin scaffolds biopolymers collagen chitosan cutaneous cells Biology (General) QH301-705.5 Chemistry QD1-999 Beata Kaczmarek-Szczepańska Justyna Ostrowska Justyna Kozłowska Zofia Szota Anna A. Brożyna Rita Dreier Russel J. Reiter Andrzej T. Slominski Kerstin Steinbrink Konrad Kleszczyński Evaluation of Polymeric Matrix Loaded with Melatonin for Wound Dressing |
topic_facet |
melatonin scaffolds biopolymers collagen chitosan cutaneous cells Biology (General) QH301-705.5 Chemistry QD1-999 |
description |
The development of scaffolds mimicking the extracellular matrix containing bioactive substances has great potential in tissue engineering and wound healing applications. This study investigates melatonin—a methoxyindole present in almost all biological systems. Melatonin is a bioregulator in terms of its potential clinical importance for future therapies of cutaneous diseases. Mammalian skin is not only a prominent melatonin target, but also produces and rapidly metabolizes the multifunctional methoxyindole to biologically active metabolites. In our methodology, chitosan/collagen (CTS/Coll)-contained biomaterials are blended with melatonin at different doses to fabricate biomimetic hybrid scaffolds. We use rat tail tendon- and Salmo salar fish skin-derived collagens to assess biophysical and cellular properties by ( i ) Fourier transform infrared spectroscopy—attenuated total reflectance (FTIR–ATR), ( ii ) thermogravimetric analysis (TG), ( iii ) scanning electron microscope (SEM), and ( iv ) proliferation ratio of cutaneous cells in vitro. Our results indicate that melatonin itself does not negatively affect biophysical properties of melatonin-immobilized hybrid scaffolds, but it induces a pronounced elevation of cell viability within human epidermal keratinocytes (NHEK), dermal fibroblasts (NHDF), and reference melanoma cells. These results demonstrate that this indoleamine accelerates re-epithelialization. This delivery is a promising technique for additional explorations in future dermatotherapy and protective skin medicine. |
format |
Article in Journal/Newspaper |
author |
Beata Kaczmarek-Szczepańska Justyna Ostrowska Justyna Kozłowska Zofia Szota Anna A. Brożyna Rita Dreier Russel J. Reiter Andrzej T. Slominski Kerstin Steinbrink Konrad Kleszczyński |
author_facet |
Beata Kaczmarek-Szczepańska Justyna Ostrowska Justyna Kozłowska Zofia Szota Anna A. Brożyna Rita Dreier Russel J. Reiter Andrzej T. Slominski Kerstin Steinbrink Konrad Kleszczyński |
author_sort |
Beata Kaczmarek-Szczepańska |
title |
Evaluation of Polymeric Matrix Loaded with Melatonin for Wound Dressing |
title_short |
Evaluation of Polymeric Matrix Loaded with Melatonin for Wound Dressing |
title_full |
Evaluation of Polymeric Matrix Loaded with Melatonin for Wound Dressing |
title_fullStr |
Evaluation of Polymeric Matrix Loaded with Melatonin for Wound Dressing |
title_full_unstemmed |
Evaluation of Polymeric Matrix Loaded with Melatonin for Wound Dressing |
title_sort |
evaluation of polymeric matrix loaded with melatonin for wound dressing |
publisher |
MDPI AG |
publishDate |
2021 |
url |
https://doi.org/10.3390/ijms22115658 https://doaj.org/article/f7716c1cc60043a889e10f216620025f |
genre |
Salmo salar |
genre_facet |
Salmo salar |
op_source |
International Journal of Molecular Sciences, Vol 22, Iss 5658, p 5658 (2021) |
op_relation |
https://www.mdpi.com/1422-0067/22/11/5658 https://doaj.org/toc/1661-6596 https://doaj.org/toc/1422-0067 doi:10.3390/ijms22115658 1422-0067 1661-6596 https://doaj.org/article/f7716c1cc60043a889e10f216620025f |
op_doi |
https://doi.org/10.3390/ijms22115658 |
container_title |
International Journal of Molecular Sciences |
container_volume |
22 |
container_issue |
11 |
container_start_page |
5658 |
_version_ |
1766182582253256704 |