Characterization of miRNAs in Extracellular Vesicles Released From Atlantic Salmon Monocyte-Like and Macrophage-Like Cells
Cell-derived extracellular vesicles (EVs) participate in cell-cell communication via transfer of molecular cargo including genetic material like miRNAs. In mammals, it has previously been established that EV-mediated transfer of miRNAs can alter the development or function of immune cells, such as m...
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Online Access: | https://doi.org/10.3389/fimmu.2020.587931 https://doaj.org/article/f758101d7eb444e9b24c1a159dab85da |
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ftdoajarticles:oai:doaj.org/article:f758101d7eb444e9b24c1a159dab85da 2023-05-15T15:30:31+02:00 Characterization of miRNAs in Extracellular Vesicles Released From Atlantic Salmon Monocyte-Like and Macrophage-Like Cells Nicole C. Smith Gabriel Wajnberg Simi Chacko Nardos T. Woldemariam Jacynthe Lacroix Nicolas Crapoulet D. Craig Ayre Stephen M. Lewis Matthew L. Rise Rune Andreassen Sherri L. Christian 2020-11-01T00:00:00Z https://doi.org/10.3389/fimmu.2020.587931 https://doaj.org/article/f758101d7eb444e9b24c1a159dab85da EN eng Frontiers Media S.A. https://www.frontiersin.org/articles/10.3389/fimmu.2020.587931/full https://doaj.org/toc/1664-3224 1664-3224 doi:10.3389/fimmu.2020.587931 https://doaj.org/article/f758101d7eb444e9b24c1a159dab85da Frontiers in Immunology, Vol 11 (2020) extracellular vesicles microRNA Atlantic salmon RNA-sequencing RNA-seq macrophage Immunologic diseases. Allergy RC581-607 article 2020 ftdoajarticles https://doi.org/10.3389/fimmu.2020.587931 2022-12-31T05:02:49Z Cell-derived extracellular vesicles (EVs) participate in cell-cell communication via transfer of molecular cargo including genetic material like miRNAs. In mammals, it has previously been established that EV-mediated transfer of miRNAs can alter the development or function of immune cells, such as macrophages. Our previous research revealed that Atlantic salmon head kidney leukocytes (HKLs) change their morphology, phagocytic ability and miRNA profile from primarily “monocyte-like” at Day 1 to primarily “macrophage-like” at Day 5 of culture. Therefore, we aimed to characterize the miRNA cargo packaged in EVs released from these two cell populations. We successfully isolated EVs from Atlantic salmon HKL culture supernatants using the established Vn96 peptide-based pull-down. Isolation was validated using transmission electron microscopy, nanoparticle tracking analysis, and Western blotting. RNA-sequencing identified 19 differentially enriched (DE) miRNAs packaged in Day 1 versus Day 5 EVs. Several of the highly abundant miRNAs, including those that were DE (e.g. ssa-miR-146a, ssa-miR-155 and ssa-miR-731), were previously identified as DE in HKLs and are associated with macrophage differentiation and immune response in other species. Interestingly, the abundance relative of the miRNAs in EVs, including the most abundant miRNA (ssa-miR-125b), was different than the miRNA abundance in HKLs, indicating selective packaging of miRNAs in EVs. Further study of the miRNA cargo in EVs derived from fish immune cells will be an important next step in identifying EV biomarkers useful for evaluating immune cell function, fish health, or response to disease. Article in Journal/Newspaper Atlantic salmon Directory of Open Access Journals: DOAJ Articles Frontiers in Immunology 11 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
extracellular vesicles microRNA Atlantic salmon RNA-sequencing RNA-seq macrophage Immunologic diseases. Allergy RC581-607 |
spellingShingle |
extracellular vesicles microRNA Atlantic salmon RNA-sequencing RNA-seq macrophage Immunologic diseases. Allergy RC581-607 Nicole C. Smith Gabriel Wajnberg Simi Chacko Nardos T. Woldemariam Jacynthe Lacroix Nicolas Crapoulet D. Craig Ayre Stephen M. Lewis Matthew L. Rise Rune Andreassen Sherri L. Christian Characterization of miRNAs in Extracellular Vesicles Released From Atlantic Salmon Monocyte-Like and Macrophage-Like Cells |
topic_facet |
extracellular vesicles microRNA Atlantic salmon RNA-sequencing RNA-seq macrophage Immunologic diseases. Allergy RC581-607 |
description |
Cell-derived extracellular vesicles (EVs) participate in cell-cell communication via transfer of molecular cargo including genetic material like miRNAs. In mammals, it has previously been established that EV-mediated transfer of miRNAs can alter the development or function of immune cells, such as macrophages. Our previous research revealed that Atlantic salmon head kidney leukocytes (HKLs) change their morphology, phagocytic ability and miRNA profile from primarily “monocyte-like” at Day 1 to primarily “macrophage-like” at Day 5 of culture. Therefore, we aimed to characterize the miRNA cargo packaged in EVs released from these two cell populations. We successfully isolated EVs from Atlantic salmon HKL culture supernatants using the established Vn96 peptide-based pull-down. Isolation was validated using transmission electron microscopy, nanoparticle tracking analysis, and Western blotting. RNA-sequencing identified 19 differentially enriched (DE) miRNAs packaged in Day 1 versus Day 5 EVs. Several of the highly abundant miRNAs, including those that were DE (e.g. ssa-miR-146a, ssa-miR-155 and ssa-miR-731), were previously identified as DE in HKLs and are associated with macrophage differentiation and immune response in other species. Interestingly, the abundance relative of the miRNAs in EVs, including the most abundant miRNA (ssa-miR-125b), was different than the miRNA abundance in HKLs, indicating selective packaging of miRNAs in EVs. Further study of the miRNA cargo in EVs derived from fish immune cells will be an important next step in identifying EV biomarkers useful for evaluating immune cell function, fish health, or response to disease. |
format |
Article in Journal/Newspaper |
author |
Nicole C. Smith Gabriel Wajnberg Simi Chacko Nardos T. Woldemariam Jacynthe Lacroix Nicolas Crapoulet D. Craig Ayre Stephen M. Lewis Matthew L. Rise Rune Andreassen Sherri L. Christian |
author_facet |
Nicole C. Smith Gabriel Wajnberg Simi Chacko Nardos T. Woldemariam Jacynthe Lacroix Nicolas Crapoulet D. Craig Ayre Stephen M. Lewis Matthew L. Rise Rune Andreassen Sherri L. Christian |
author_sort |
Nicole C. Smith |
title |
Characterization of miRNAs in Extracellular Vesicles Released From Atlantic Salmon Monocyte-Like and Macrophage-Like Cells |
title_short |
Characterization of miRNAs in Extracellular Vesicles Released From Atlantic Salmon Monocyte-Like and Macrophage-Like Cells |
title_full |
Characterization of miRNAs in Extracellular Vesicles Released From Atlantic Salmon Monocyte-Like and Macrophage-Like Cells |
title_fullStr |
Characterization of miRNAs in Extracellular Vesicles Released From Atlantic Salmon Monocyte-Like and Macrophage-Like Cells |
title_full_unstemmed |
Characterization of miRNAs in Extracellular Vesicles Released From Atlantic Salmon Monocyte-Like and Macrophage-Like Cells |
title_sort |
characterization of mirnas in extracellular vesicles released from atlantic salmon monocyte-like and macrophage-like cells |
publisher |
Frontiers Media S.A. |
publishDate |
2020 |
url |
https://doi.org/10.3389/fimmu.2020.587931 https://doaj.org/article/f758101d7eb444e9b24c1a159dab85da |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_source |
Frontiers in Immunology, Vol 11 (2020) |
op_relation |
https://www.frontiersin.org/articles/10.3389/fimmu.2020.587931/full https://doaj.org/toc/1664-3224 1664-3224 doi:10.3389/fimmu.2020.587931 https://doaj.org/article/f758101d7eb444e9b24c1a159dab85da |
op_doi |
https://doi.org/10.3389/fimmu.2020.587931 |
container_title |
Frontiers in Immunology |
container_volume |
11 |
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1766360968231649280 |