Response surface methodology-based optimization of ultrasound-assisted extraction of β-sitosterol and lupeol from astragalus atropilosus (roots) and validation by HPTLC method
Objective: To optimize the ultrasonication method for efficient extraction of P-sitosterol and lupeol from the roots of Astragalus atropilosus using Box-Behnken design of response surface methodology (RSM), and its validation by high performance thin layer chromatography (HPTLC) method. Methods: Ult...
Published in: | Asian Pacific Journal of Tropical Biomedicine |
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Language: | English |
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Wolters Kluwer Medknow Publications
2020
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Online Access: | https://doi.org/10.4103/2221-1691.283942 https://doaj.org/article/f729d3afac1a479b94fc36fc27f3a199 |
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ftdoajarticles:oai:doaj.org/article:f729d3afac1a479b94fc36fc27f3a199 2023-05-15T15:19:12+02:00 Response surface methodology-based optimization of ultrasound-assisted extraction of β-sitosterol and lupeol from astragalus atropilosus (roots) and validation by HPTLC method Perwez Alam Nasir A Siddiqui Ali S Alqahtani Anzarul Haque Omer A Basudan Saleh I Alqasoumi Abdullah A AL-Mishari M U Khan 2020-01-01T00:00:00Z https://doi.org/10.4103/2221-1691.283942 https://doaj.org/article/f729d3afac1a479b94fc36fc27f3a199 EN eng Wolters Kluwer Medknow Publications http://www.apjtb.org/article.asp?issn=2221-1691;year=2020;volume=10;issue=6;spage=281;epage=292;aulast=Alam https://doaj.org/toc/2221-1691 https://doaj.org/toc/2588-9222 2221-1691 2588-9222 doi:10.4103/2221-1691.283942 https://doaj.org/article/f729d3afac1a479b94fc36fc27f3a199 Asian Pacific Journal of Tropical Biomedicine, Vol 10, Iss 6, Pp 281-292 (2020) β-sitosterol lupeol box-behnken design astragalus high performance thin layer chromatography Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2020 ftdoajarticles https://doi.org/10.4103/2221-1691.283942 2022-12-31T09:57:04Z Objective: To optimize the ultrasonication method for efficient extraction of P-sitosterol and lupeol from the roots of Astragalus atropilosus using Box-Behnken design of response surface methodology (RSM), and its validation by high performance thin layer chromatography (HPTLC) method. Methods: Ultrasonication method was used to extract β-sitosterol and lupeol from Astragalus atropilosus (roots). RSM was used to optimize the different extraction parameters viz. liquid to solid ratio (10-14 mL/g), temperature (60-80 °C) and time (40-60 min) to maximize the yield of β-sitosterol and lupeol. The quantitative estimation of β-sitosterol and lupeol was done in chloroform extract of Astragalus atropilosus by validated HPTLC method on 10 cm × 20 cm glass-backed silica gel 60F254 plate using hexane and ethyl acetate (8:2, v/v) as mobile phase. Results: A quadratic polynomial model was found to be most appropriate with regard to R1 (yield of total extraction; R2/% CV = 0.994 8/0.28), R2 (β-sitosterol yield; R2/% CV = 0.992 3/0.39) and R3 (lupeol yield; R2/% CV = 0.994 2/0.97). The values of adjusted R2/predicted R2/signal to noise ratio for R1, R2, and R 3 were 0.978 2/0.955 1/48.77, 0.990 4/0.911 0/31.33, and 0.992 7/0.940 1/36.08, respectively, indicating a high degree of correlation and adequate signal. The linear correlation plot between the predicted and experimental values for R1, R2, and R3 showed high values of R2 ranging from 0.990 5-0.997 3. β-sitosterol and lupeol in chloroform extract of Astragalus atropilosus were detected at Rf values of 0.22 and 0.34, respectively, at X max = 518 nm. The optimized ultrasonic extraction produced 8.462% w/w of Rl, 0.451% w/w of R2 and 0.172% w/w of R3 at 13.5 mL/g liquid to solid ratio, 78 C of temperature and 60 min of time. Conclusions: The experimental findings of RSM optimized extraction and HPTLC analysis can be further applied for the efficient extraction of β-sitosterol and lupeol in other species of Astragalus. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Biomedicine 10 6 281 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
β-sitosterol lupeol box-behnken design astragalus high performance thin layer chromatography Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
spellingShingle |
β-sitosterol lupeol box-behnken design astragalus high performance thin layer chromatography Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Perwez Alam Nasir A Siddiqui Ali S Alqahtani Anzarul Haque Omer A Basudan Saleh I Alqasoumi Abdullah A AL-Mishari M U Khan Response surface methodology-based optimization of ultrasound-assisted extraction of β-sitosterol and lupeol from astragalus atropilosus (roots) and validation by HPTLC method |
topic_facet |
β-sitosterol lupeol box-behnken design astragalus high performance thin layer chromatography Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To optimize the ultrasonication method for efficient extraction of P-sitosterol and lupeol from the roots of Astragalus atropilosus using Box-Behnken design of response surface methodology (RSM), and its validation by high performance thin layer chromatography (HPTLC) method. Methods: Ultrasonication method was used to extract β-sitosterol and lupeol from Astragalus atropilosus (roots). RSM was used to optimize the different extraction parameters viz. liquid to solid ratio (10-14 mL/g), temperature (60-80 °C) and time (40-60 min) to maximize the yield of β-sitosterol and lupeol. The quantitative estimation of β-sitosterol and lupeol was done in chloroform extract of Astragalus atropilosus by validated HPTLC method on 10 cm × 20 cm glass-backed silica gel 60F254 plate using hexane and ethyl acetate (8:2, v/v) as mobile phase. Results: A quadratic polynomial model was found to be most appropriate with regard to R1 (yield of total extraction; R2/% CV = 0.994 8/0.28), R2 (β-sitosterol yield; R2/% CV = 0.992 3/0.39) and R3 (lupeol yield; R2/% CV = 0.994 2/0.97). The values of adjusted R2/predicted R2/signal to noise ratio for R1, R2, and R 3 were 0.978 2/0.955 1/48.77, 0.990 4/0.911 0/31.33, and 0.992 7/0.940 1/36.08, respectively, indicating a high degree of correlation and adequate signal. The linear correlation plot between the predicted and experimental values for R1, R2, and R3 showed high values of R2 ranging from 0.990 5-0.997 3. β-sitosterol and lupeol in chloroform extract of Astragalus atropilosus were detected at Rf values of 0.22 and 0.34, respectively, at X max = 518 nm. The optimized ultrasonic extraction produced 8.462% w/w of Rl, 0.451% w/w of R2 and 0.172% w/w of R3 at 13.5 mL/g liquid to solid ratio, 78 C of temperature and 60 min of time. Conclusions: The experimental findings of RSM optimized extraction and HPTLC analysis can be further applied for the efficient extraction of β-sitosterol and lupeol in other species of Astragalus. |
format |
Article in Journal/Newspaper |
author |
Perwez Alam Nasir A Siddiqui Ali S Alqahtani Anzarul Haque Omer A Basudan Saleh I Alqasoumi Abdullah A AL-Mishari M U Khan |
author_facet |
Perwez Alam Nasir A Siddiqui Ali S Alqahtani Anzarul Haque Omer A Basudan Saleh I Alqasoumi Abdullah A AL-Mishari M U Khan |
author_sort |
Perwez Alam |
title |
Response surface methodology-based optimization of ultrasound-assisted extraction of β-sitosterol and lupeol from astragalus atropilosus (roots) and validation by HPTLC method |
title_short |
Response surface methodology-based optimization of ultrasound-assisted extraction of β-sitosterol and lupeol from astragalus atropilosus (roots) and validation by HPTLC method |
title_full |
Response surface methodology-based optimization of ultrasound-assisted extraction of β-sitosterol and lupeol from astragalus atropilosus (roots) and validation by HPTLC method |
title_fullStr |
Response surface methodology-based optimization of ultrasound-assisted extraction of β-sitosterol and lupeol from astragalus atropilosus (roots) and validation by HPTLC method |
title_full_unstemmed |
Response surface methodology-based optimization of ultrasound-assisted extraction of β-sitosterol and lupeol from astragalus atropilosus (roots) and validation by HPTLC method |
title_sort |
response surface methodology-based optimization of ultrasound-assisted extraction of β-sitosterol and lupeol from astragalus atropilosus (roots) and validation by hptlc method |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2020 |
url |
https://doi.org/10.4103/2221-1691.283942 https://doaj.org/article/f729d3afac1a479b94fc36fc27f3a199 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 10, Iss 6, Pp 281-292 (2020) |
op_relation |
http://www.apjtb.org/article.asp?issn=2221-1691;year=2020;volume=10;issue=6;spage=281;epage=292;aulast=Alam https://doaj.org/toc/2221-1691 https://doaj.org/toc/2588-9222 2221-1691 2588-9222 doi:10.4103/2221-1691.283942 https://doaj.org/article/f729d3afac1a479b94fc36fc27f3a199 |
op_doi |
https://doi.org/10.4103/2221-1691.283942 |
container_title |
Asian Pacific Journal of Tropical Biomedicine |
container_volume |
10 |
container_issue |
6 |
container_start_page |
281 |
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1766349389354237952 |