In vitro studies to assess the antioxidative, radical scavenging and arginase inhibitory potentials of extracts from Artocarpus altilis, Ficus exasperate and Kigelia africana
Objective: To justify the use of Artocarpus altilis (A. altilis), Ficus exasperata (F. exasperata) and Kigelia africana (K. africana) in ethnomedicine for the treatment of several ailments and to evaluate the in vitro antioxidant, radical scavenging and arginase inhibitory potentials of these herbs...
Published in: | Asian Pacific Journal of Tropical Biomedicine |
---|---|
Main Authors: | , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Wolters Kluwer Medknow Publications
2014
|
Subjects: | |
Online Access: | https://doi.org/10.12980/APJTB.4.2014C581 https://doaj.org/article/f721eff740974faa88296be0e2d82353 |
id |
ftdoajarticles:oai:doaj.org/article:f721eff740974faa88296be0e2d82353 |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:f721eff740974faa88296be0e2d82353 2023-05-15T15:15:04+02:00 In vitro studies to assess the antioxidative, radical scavenging and arginase inhibitory potentials of extracts from Artocarpus altilis, Ficus exasperate and Kigelia africana Olubukola Oyebimpe Akanni Solomon Eduviere Owumi Oluwatosin Adekunle Adaramoye 2014-05-01T00:00:00Z https://doi.org/10.12980/APJTB.4.2014C581 https://doaj.org/article/f721eff740974faa88296be0e2d82353 EN eng Wolters Kluwer Medknow Publications http://www.sciencedirect.com/science/article/pii/S2221169115303166 https://doaj.org/toc/2221-1691 2221-1691 doi:10.12980/APJTB.4.2014C581 https://doaj.org/article/f721eff740974faa88296be0e2d82353 Asian Pacific Journal of Tropical Biomedicine, Vol 4, Iss S1, Pp S492-S499 (2014) Antioxidant lipid peroxidation Arginase Free radicals Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2014 ftdoajarticles https://doi.org/10.12980/APJTB.4.2014C581 2022-12-31T12:14:54Z Objective: To justify the use of Artocarpus altilis (A. altilis), Ficus exasperata (F. exasperata) and Kigelia africana (K. africana) in ethnomedicine for the treatment of several ailments and to evaluate the in vitro antioxidant, radical scavenging and arginase inhibitory potentials of these herbs and compared with catechin (Standard). Methods: Antioxidant activities were determined by 2,2-diphenyl-1-picrylhydrazyl (DPPH), nitric oxide, hydrogen peroxide (H2O2) and hydroxyl (OH) radicals scavenging methods. The flavonoids and phenolics content, inhibition of arginase activity, Fe2+/ascorbate-induced lipid peroxidation (LPO) and reducing power were also determined. Results: The A. altilis, F. exasperata and K. africana showed dose-dependent and significant scavenging of DPPH, H2O2 and OH radicals in vitro relative to catechin. The A. altilis and F. exasperata effectively scavenged DPPH radical with IC50 of 593 and 635 μg/mL and, OH radical with IC50 of 487 and 514 μg/mL, respectively. The DPPH and OH radicals scavenging activities followed the order A. altilis>F. exasperata>K. africana. In addition, A. altilis and F. exasperata significantly (P<0.05) inhibited LPO in a dose-dependent manner. The A. altilis extract had the most potent inhibitory activity against LPO with 79% relative to catechin (28%) at 750 μg/mL. The reducing power followed the order: A. altilis>Catechin>F. exasperata>K. africana at 1 000 μg/mL. The A. altilis at 500 and 750 μg/mL significantly (P<0.05) inhibited arginase activity by 63% and 67%, respectively. The flavonoids contents were found to be highest in A. altilis. Conclusions: Extracts of A. altilis and F. exasperata are potent antioxidative agents with strong radical scavenging activity and inhibition of lipid peroxidation. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Biomedicine 4 S492 S499 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Antioxidant lipid peroxidation Arginase Free radicals Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
spellingShingle |
Antioxidant lipid peroxidation Arginase Free radicals Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Olubukola Oyebimpe Akanni Solomon Eduviere Owumi Oluwatosin Adekunle Adaramoye In vitro studies to assess the antioxidative, radical scavenging and arginase inhibitory potentials of extracts from Artocarpus altilis, Ficus exasperate and Kigelia africana |
topic_facet |
Antioxidant lipid peroxidation Arginase Free radicals Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To justify the use of Artocarpus altilis (A. altilis), Ficus exasperata (F. exasperata) and Kigelia africana (K. africana) in ethnomedicine for the treatment of several ailments and to evaluate the in vitro antioxidant, radical scavenging and arginase inhibitory potentials of these herbs and compared with catechin (Standard). Methods: Antioxidant activities were determined by 2,2-diphenyl-1-picrylhydrazyl (DPPH), nitric oxide, hydrogen peroxide (H2O2) and hydroxyl (OH) radicals scavenging methods. The flavonoids and phenolics content, inhibition of arginase activity, Fe2+/ascorbate-induced lipid peroxidation (LPO) and reducing power were also determined. Results: The A. altilis, F. exasperata and K. africana showed dose-dependent and significant scavenging of DPPH, H2O2 and OH radicals in vitro relative to catechin. The A. altilis and F. exasperata effectively scavenged DPPH radical with IC50 of 593 and 635 μg/mL and, OH radical with IC50 of 487 and 514 μg/mL, respectively. The DPPH and OH radicals scavenging activities followed the order A. altilis>F. exasperata>K. africana. In addition, A. altilis and F. exasperata significantly (P<0.05) inhibited LPO in a dose-dependent manner. The A. altilis extract had the most potent inhibitory activity against LPO with 79% relative to catechin (28%) at 750 μg/mL. The reducing power followed the order: A. altilis>Catechin>F. exasperata>K. africana at 1 000 μg/mL. The A. altilis at 500 and 750 μg/mL significantly (P<0.05) inhibited arginase activity by 63% and 67%, respectively. The flavonoids contents were found to be highest in A. altilis. Conclusions: Extracts of A. altilis and F. exasperata are potent antioxidative agents with strong radical scavenging activity and inhibition of lipid peroxidation. |
format |
Article in Journal/Newspaper |
author |
Olubukola Oyebimpe Akanni Solomon Eduviere Owumi Oluwatosin Adekunle Adaramoye |
author_facet |
Olubukola Oyebimpe Akanni Solomon Eduviere Owumi Oluwatosin Adekunle Adaramoye |
author_sort |
Olubukola Oyebimpe Akanni |
title |
In vitro studies to assess the antioxidative, radical scavenging and arginase inhibitory potentials of extracts from Artocarpus altilis, Ficus exasperate and Kigelia africana |
title_short |
In vitro studies to assess the antioxidative, radical scavenging and arginase inhibitory potentials of extracts from Artocarpus altilis, Ficus exasperate and Kigelia africana |
title_full |
In vitro studies to assess the antioxidative, radical scavenging and arginase inhibitory potentials of extracts from Artocarpus altilis, Ficus exasperate and Kigelia africana |
title_fullStr |
In vitro studies to assess the antioxidative, radical scavenging and arginase inhibitory potentials of extracts from Artocarpus altilis, Ficus exasperate and Kigelia africana |
title_full_unstemmed |
In vitro studies to assess the antioxidative, radical scavenging and arginase inhibitory potentials of extracts from Artocarpus altilis, Ficus exasperate and Kigelia africana |
title_sort |
in vitro studies to assess the antioxidative, radical scavenging and arginase inhibitory potentials of extracts from artocarpus altilis, ficus exasperate and kigelia africana |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2014 |
url |
https://doi.org/10.12980/APJTB.4.2014C581 https://doaj.org/article/f721eff740974faa88296be0e2d82353 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 4, Iss S1, Pp S492-S499 (2014) |
op_relation |
http://www.sciencedirect.com/science/article/pii/S2221169115303166 https://doaj.org/toc/2221-1691 2221-1691 doi:10.12980/APJTB.4.2014C581 https://doaj.org/article/f721eff740974faa88296be0e2d82353 |
op_doi |
https://doi.org/10.12980/APJTB.4.2014C581 |
container_title |
Asian Pacific Journal of Tropical Biomedicine |
container_volume |
4 |
container_start_page |
S492 |
op_container_end_page |
S499 |
_version_ |
1766345445898977280 |