IL35 modulation altered survival, cytokine environment and histopathological consequences during malaria infection in mice
Abstract Background The immune modulating potential of IL-35 in multiple human disorders has been reported. Consequent upon the recognition of inflammatory cytokine activation and its preponderance for mediating pathology during malaria infection, the study aimed to characterize the expression and f...
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ftdoajarticles:oai:doaj.org/article:f70af4ee52184b678ef5545d17ed0fa5 2023-05-15T15:12:28+02:00 IL35 modulation altered survival, cytokine environment and histopathological consequences during malaria infection in mice Ramatu Omenesa Bello Maizaton Atmadini Abdullah Roslaini Abd Majid Voon Kin Chin Mohammed Faruq Abd Rachman Isnadi Zaid Osama Ibraheem Mohd Khairi Hussain Mohammed Garba Magaji Rusliza Basir 2019-12-01T00:00:00Z https://doi.org/10.1186/s12936-019-3070-x https://doaj.org/article/f70af4ee52184b678ef5545d17ed0fa5 EN eng BMC https://doi.org/10.1186/s12936-019-3070-x https://doaj.org/toc/1475-2875 doi:10.1186/s12936-019-3070-x 1475-2875 https://doaj.org/article/f70af4ee52184b678ef5545d17ed0fa5 Malaria Journal, Vol 18, Iss 1, Pp 1-23 (2019) Plasmodium berghei Interleukin-35 Immunohistochemistry Cytokines Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2019 ftdoajarticles https://doi.org/10.1186/s12936-019-3070-x 2022-12-31T06:06:35Z Abstract Background The immune modulating potential of IL-35 in multiple human disorders has been reported. Consequent upon the recognition of inflammatory cytokine activation and its preponderance for mediating pathology during malaria infection, the study aimed to characterize the expression and functional contribution(s) of IL-35 in Plasmodium berghei (strain ANKA) infected mice. Methods Plasmodium berghei infection in male ICR mice was used as the rodent model of choice. The time course of IL-35 expression in the systemic circulation and tissues of P. berghei infected mice as well as their healthy control counterparts was assessed by enzyme linked immunosorbent assay and immunohistochemistry respectively. The effect of modulating IL-35 by recombinant IL-35 protein or neutralizing anti-Epstein-Barr virus-induced gene 3 antibody on the cytokine environment during P. berghei infection was assessed by flow cytometry. Furthermore, the influence of modulating IL-35 on histopathological hallmarks of malaria and disease progression was evaluated. Results Interleukin-35 was significantly up regulated in serum and tissues of P. berghei infected mice and correlated with parasitaemia. Neutralization of IL-35 significantly enhanced the release of IFN-γ, decreased the expression of IL-6 and decreased parasitaemia patency. Neutralization of IL-35 was also associated with a tendency towards increased survival as well as the absence of pathological features associated with malaria infection unlike recombinant IL-35 protein administration which sustained a normal course of infection and unfavourable malaria associated histological outcomes in P. berghei infected mice. Conclusion These results indicate the involvement of IL-35 in P. berghei induced malaria infection. IL-35 neutralization strategies may represent viable therapeutic modalities beneficial for the resolution of malaria infection. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 18 1 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
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English |
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Plasmodium berghei Interleukin-35 Immunohistochemistry Cytokines Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
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Plasmodium berghei Interleukin-35 Immunohistochemistry Cytokines Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Ramatu Omenesa Bello Maizaton Atmadini Abdullah Roslaini Abd Majid Voon Kin Chin Mohammed Faruq Abd Rachman Isnadi Zaid Osama Ibraheem Mohd Khairi Hussain Mohammed Garba Magaji Rusliza Basir IL35 modulation altered survival, cytokine environment and histopathological consequences during malaria infection in mice |
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Plasmodium berghei Interleukin-35 Immunohistochemistry Cytokines Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background The immune modulating potential of IL-35 in multiple human disorders has been reported. Consequent upon the recognition of inflammatory cytokine activation and its preponderance for mediating pathology during malaria infection, the study aimed to characterize the expression and functional contribution(s) of IL-35 in Plasmodium berghei (strain ANKA) infected mice. Methods Plasmodium berghei infection in male ICR mice was used as the rodent model of choice. The time course of IL-35 expression in the systemic circulation and tissues of P. berghei infected mice as well as their healthy control counterparts was assessed by enzyme linked immunosorbent assay and immunohistochemistry respectively. The effect of modulating IL-35 by recombinant IL-35 protein or neutralizing anti-Epstein-Barr virus-induced gene 3 antibody on the cytokine environment during P. berghei infection was assessed by flow cytometry. Furthermore, the influence of modulating IL-35 on histopathological hallmarks of malaria and disease progression was evaluated. Results Interleukin-35 was significantly up regulated in serum and tissues of P. berghei infected mice and correlated with parasitaemia. Neutralization of IL-35 significantly enhanced the release of IFN-γ, decreased the expression of IL-6 and decreased parasitaemia patency. Neutralization of IL-35 was also associated with a tendency towards increased survival as well as the absence of pathological features associated with malaria infection unlike recombinant IL-35 protein administration which sustained a normal course of infection and unfavourable malaria associated histological outcomes in P. berghei infected mice. Conclusion These results indicate the involvement of IL-35 in P. berghei induced malaria infection. IL-35 neutralization strategies may represent viable therapeutic modalities beneficial for the resolution of malaria infection. |
format |
Article in Journal/Newspaper |
author |
Ramatu Omenesa Bello Maizaton Atmadini Abdullah Roslaini Abd Majid Voon Kin Chin Mohammed Faruq Abd Rachman Isnadi Zaid Osama Ibraheem Mohd Khairi Hussain Mohammed Garba Magaji Rusliza Basir |
author_facet |
Ramatu Omenesa Bello Maizaton Atmadini Abdullah Roslaini Abd Majid Voon Kin Chin Mohammed Faruq Abd Rachman Isnadi Zaid Osama Ibraheem Mohd Khairi Hussain Mohammed Garba Magaji Rusliza Basir |
author_sort |
Ramatu Omenesa Bello |
title |
IL35 modulation altered survival, cytokine environment and histopathological consequences during malaria infection in mice |
title_short |
IL35 modulation altered survival, cytokine environment and histopathological consequences during malaria infection in mice |
title_full |
IL35 modulation altered survival, cytokine environment and histopathological consequences during malaria infection in mice |
title_fullStr |
IL35 modulation altered survival, cytokine environment and histopathological consequences during malaria infection in mice |
title_full_unstemmed |
IL35 modulation altered survival, cytokine environment and histopathological consequences during malaria infection in mice |
title_sort |
il35 modulation altered survival, cytokine environment and histopathological consequences during malaria infection in mice |
publisher |
BMC |
publishDate |
2019 |
url |
https://doi.org/10.1186/s12936-019-3070-x https://doaj.org/article/f70af4ee52184b678ef5545d17ed0fa5 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 18, Iss 1, Pp 1-23 (2019) |
op_relation |
https://doi.org/10.1186/s12936-019-3070-x https://doaj.org/toc/1475-2875 doi:10.1186/s12936-019-3070-x 1475-2875 https://doaj.org/article/f70af4ee52184b678ef5545d17ed0fa5 |
op_doi |
https://doi.org/10.1186/s12936-019-3070-x |
container_title |
Malaria Journal |
container_volume |
18 |
container_issue |
1 |
_version_ |
1766343143747223552 |