Preliminary results with a torsion microbalance indicate that carbon dioxide and exposed carbonic anhydrase in the organic matrix are the basis of calcification on the skeleton surface of living corals
Ocean acidification is altering the calcification of corals, but the mechanism is still unclear. To explore what controls calcification, small pieces from the edges of thin plates of Agaricia agaricites were suspended from a torsion microbalance into gently stirred, temperaturecontrolled, seawater....
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Universidad de Costa Rica
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ftdoajarticles:oai:doaj.org/article:f70174b85be847da9223f2ed74ff85f4 2024-09-15T18:28:28+00:00 Preliminary results with a torsion microbalance indicate that carbon dioxide and exposed carbonic anhydrase in the organic matrix are the basis of calcification on the skeleton surface of living corals Ian M Sandeman 2012-03-01T00:00:00Z https://doaj.org/article/f70174b85be847da9223f2ed74ff85f4 EN ES eng spa Universidad de Costa Rica http://www.scielo.sa.cr/scielo.php?script=sci_arttext&pid=S0034-77442012000500010 https://doaj.org/toc/0034-7744 https://doaj.org/toc/2215-2075 0034-7744 2215-2075 https://doaj.org/article/f70174b85be847da9223f2ed74ff85f4 Revista de Biología Tropical, Vol 60, Pp 109-126 (2012) calcificación de corales CO2 pH temperatura matriz orgánica anhidrasa carbónica coral calcification temperature organic matrix carbonic anhydrase Biology (General) QH301-705.5 article 2012 ftdoajarticles 2024-08-05T17:48:33Z Ocean acidification is altering the calcification of corals, but the mechanism is still unclear. To explore what controls calcification, small pieces from the edges of thin plates of Agaricia agaricites were suspended from a torsion microbalance into gently stirred, temperaturecontrolled, seawater. Net calcification rates were monitored while light, temperature and pH were manipulated singly. The living coral pieces were sensitive to changes in conditions, especially light, and calcification was often suspended for one or two hours or overnight. The mean calcification rate increased from 0.06 in the dark to 0.10 mg.h-1.cm-2 (T test, n=8, p<0.01) in low light (15 μmol.s-1.m-2) and showed a positive linear relationship with temperature. With a reduction of mean pH from 8.2 to 7.6 the mean calcification rate in the light (65 μmol.s-1.m-2) increased from 0.19 to 0.28 mg.h-1.cm-2 (T test, n=8, p<0.05) indicating a dependency on carbon dioxide. After waterpiking and exposure of the skeletal surface/organic matrix to seawater, calcification showed an astonishing initial increase of more than an order of magnitude then decreased following a non-linear generalised Michaelis-Menten growth curve and reached a steady rate. Calcification rate of the freshly waterpiked coral was not influenced by light and was positively correlated with temperature. For a mean pH reduction from 8.1 to 7.6 the mean calcification rate increased from 0.18 to 0.32 mg.h-1.cm-2 (T test, n=11, p<0.02) again indicating a dependency on carbon dioxide. Calcification ceased in the presence of the carbonic anhydrase inhibitor azolamide. Staining confirmed the presence of carbonic anhydrase, particularly on the ridges of septae. After immersion of waterpiked corals in seawater for 48 hours weight gain and loss became linear and positively correlated to temperature. When the mean pH was reduced from 8.2 to 7.5 the mean rate of weight gain decreased from 0.25 to 0.13 mg.h-1.cm-2 (T test, n=6, p<0.05) indicating a dependence on carbonate. At a pH ... Article in Journal/Newspaper Ocean acidification Directory of Open Access Journals: DOAJ Articles |
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calcificación de corales CO2 pH temperatura matriz orgánica anhidrasa carbónica coral calcification temperature organic matrix carbonic anhydrase Biology (General) QH301-705.5 |
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calcificación de corales CO2 pH temperatura matriz orgánica anhidrasa carbónica coral calcification temperature organic matrix carbonic anhydrase Biology (General) QH301-705.5 Ian M Sandeman Preliminary results with a torsion microbalance indicate that carbon dioxide and exposed carbonic anhydrase in the organic matrix are the basis of calcification on the skeleton surface of living corals |
topic_facet |
calcificación de corales CO2 pH temperatura matriz orgánica anhidrasa carbónica coral calcification temperature organic matrix carbonic anhydrase Biology (General) QH301-705.5 |
description |
Ocean acidification is altering the calcification of corals, but the mechanism is still unclear. To explore what controls calcification, small pieces from the edges of thin plates of Agaricia agaricites were suspended from a torsion microbalance into gently stirred, temperaturecontrolled, seawater. Net calcification rates were monitored while light, temperature and pH were manipulated singly. The living coral pieces were sensitive to changes in conditions, especially light, and calcification was often suspended for one or two hours or overnight. The mean calcification rate increased from 0.06 in the dark to 0.10 mg.h-1.cm-2 (T test, n=8, p<0.01) in low light (15 μmol.s-1.m-2) and showed a positive linear relationship with temperature. With a reduction of mean pH from 8.2 to 7.6 the mean calcification rate in the light (65 μmol.s-1.m-2) increased from 0.19 to 0.28 mg.h-1.cm-2 (T test, n=8, p<0.05) indicating a dependency on carbon dioxide. After waterpiking and exposure of the skeletal surface/organic matrix to seawater, calcification showed an astonishing initial increase of more than an order of magnitude then decreased following a non-linear generalised Michaelis-Menten growth curve and reached a steady rate. Calcification rate of the freshly waterpiked coral was not influenced by light and was positively correlated with temperature. For a mean pH reduction from 8.1 to 7.6 the mean calcification rate increased from 0.18 to 0.32 mg.h-1.cm-2 (T test, n=11, p<0.02) again indicating a dependency on carbon dioxide. Calcification ceased in the presence of the carbonic anhydrase inhibitor azolamide. Staining confirmed the presence of carbonic anhydrase, particularly on the ridges of septae. After immersion of waterpiked corals in seawater for 48 hours weight gain and loss became linear and positively correlated to temperature. When the mean pH was reduced from 8.2 to 7.5 the mean rate of weight gain decreased from 0.25 to 0.13 mg.h-1.cm-2 (T test, n=6, p<0.05) indicating a dependence on carbonate. At a pH ... |
format |
Article in Journal/Newspaper |
author |
Ian M Sandeman |
author_facet |
Ian M Sandeman |
author_sort |
Ian M Sandeman |
title |
Preliminary results with a torsion microbalance indicate that carbon dioxide and exposed carbonic anhydrase in the organic matrix are the basis of calcification on the skeleton surface of living corals |
title_short |
Preliminary results with a torsion microbalance indicate that carbon dioxide and exposed carbonic anhydrase in the organic matrix are the basis of calcification on the skeleton surface of living corals |
title_full |
Preliminary results with a torsion microbalance indicate that carbon dioxide and exposed carbonic anhydrase in the organic matrix are the basis of calcification on the skeleton surface of living corals |
title_fullStr |
Preliminary results with a torsion microbalance indicate that carbon dioxide and exposed carbonic anhydrase in the organic matrix are the basis of calcification on the skeleton surface of living corals |
title_full_unstemmed |
Preliminary results with a torsion microbalance indicate that carbon dioxide and exposed carbonic anhydrase in the organic matrix are the basis of calcification on the skeleton surface of living corals |
title_sort |
preliminary results with a torsion microbalance indicate that carbon dioxide and exposed carbonic anhydrase in the organic matrix are the basis of calcification on the skeleton surface of living corals |
publisher |
Universidad de Costa Rica |
publishDate |
2012 |
url |
https://doaj.org/article/f70174b85be847da9223f2ed74ff85f4 |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_source |
Revista de Biología Tropical, Vol 60, Pp 109-126 (2012) |
op_relation |
http://www.scielo.sa.cr/scielo.php?script=sci_arttext&pid=S0034-77442012000500010 https://doaj.org/toc/0034-7744 https://doaj.org/toc/2215-2075 0034-7744 2215-2075 https://doaj.org/article/f70174b85be847da9223f2ed74ff85f4 |
_version_ |
1810469846430777344 |