Development of Equine IgG Antivenoms against Major Snake Groups in Mozambique.
BACKGROUND:Snake envenoming is a significant public health problem in underdeveloped and developing countries. In sub-Saharan Africa, it is estimated that 90,000-400,000 envenomations occur each year, resulting in 3,500-32,000 deaths. Envenomings are caused by snakes from the Viperidae (Bitis spp. a...
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ftdoajarticles:oai:doaj.org/article:f6ee1c4cc7b14e1290f44740b47651df 2023-05-15T15:16:35+02:00 Development of Equine IgG Antivenoms against Major Snake Groups in Mozambique. Felipe Raimondi Guidolin Celso Pereira Caricati José Roberto Marcelino Wilmar Dias da Silva 2016-01-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0004325 https://doaj.org/article/f6ee1c4cc7b14e1290f44740b47651df EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC4701360?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0004325 https://doaj.org/article/f6ee1c4cc7b14e1290f44740b47651df PLoS Neglected Tropical Diseases, Vol 10, Iss 1, p e0004325 (2016) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2016 ftdoajarticles https://doi.org/10.1371/journal.pntd.0004325 2022-12-31T08:17:05Z BACKGROUND:Snake envenoming is a significant public health problem in underdeveloped and developing countries. In sub-Saharan Africa, it is estimated that 90,000-400,000 envenomations occur each year, resulting in 3,500-32,000 deaths. Envenomings are caused by snakes from the Viperidae (Bitis spp. and Echis spp.) and Elapidae (Naja spp. and Dendroaspis spp.) families. The African continent has been suffering from a severe antivenom crisis and current antivenom production is only sufficient to treat 25% of snakebite cases. Our aim is to develop high-quality antivenoms against the main snake species found in Mozambique. METHODS:Adult horses primed with the indicated venoms were divided into 5 groups (B. arietans; B. nasicornis + B. rhinoceros; N. melanoleuca; N. mossambica; N. annulifera + D. polylepis + D. angusticeps) and reimmunized two times for antivenom production. Blood was collected, and plasma was separated and subjected to antibody purification using caprylic acid. Plasmas and antivenoms were subject to titration, affinity determination, cross-recognition assays and in vivo venom lethality neutralization. A commercial anti-Crotalic antivenom was used for comparison. RESULTS:The purified antivenoms exhibited high titers against B. arietans, B. nasicornis and B. rhinoceros (5.18 x 106, 3.60 x 106 and 3.50 x 106 U-E/mL, respectively) and N. melanoleuca, N. mossambica and N. annulifera (7.41 x 106, 3.07 x 106 and 2.60 x 106 U-E/mL, respectively), but lower titers against the D. angusticeps and D. polylepis (1.87 x 106 and 1.67 x 106 U-E/mL). All the groups, except anti-N. melanoleuca, showed significant differences from the anti-Crotalic antivenom (7.55 x 106 U-E/mL). The affinity index of all the groups was high, ranging from 31% to 45%. Cross-recognition assays showed the recognition of proteins with similar molecular weight in the venoms and may indicate the possibility of paraspecific neutralization. The three monospecific antivenoms were able to provide in vivo protection. CONCLUSION:Our results ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 10 1 e0004325 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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English |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Felipe Raimondi Guidolin Celso Pereira Caricati José Roberto Marcelino Wilmar Dias da Silva Development of Equine IgG Antivenoms against Major Snake Groups in Mozambique. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
BACKGROUND:Snake envenoming is a significant public health problem in underdeveloped and developing countries. In sub-Saharan Africa, it is estimated that 90,000-400,000 envenomations occur each year, resulting in 3,500-32,000 deaths. Envenomings are caused by snakes from the Viperidae (Bitis spp. and Echis spp.) and Elapidae (Naja spp. and Dendroaspis spp.) families. The African continent has been suffering from a severe antivenom crisis and current antivenom production is only sufficient to treat 25% of snakebite cases. Our aim is to develop high-quality antivenoms against the main snake species found in Mozambique. METHODS:Adult horses primed with the indicated venoms were divided into 5 groups (B. arietans; B. nasicornis + B. rhinoceros; N. melanoleuca; N. mossambica; N. annulifera + D. polylepis + D. angusticeps) and reimmunized two times for antivenom production. Blood was collected, and plasma was separated and subjected to antibody purification using caprylic acid. Plasmas and antivenoms were subject to titration, affinity determination, cross-recognition assays and in vivo venom lethality neutralization. A commercial anti-Crotalic antivenom was used for comparison. RESULTS:The purified antivenoms exhibited high titers against B. arietans, B. nasicornis and B. rhinoceros (5.18 x 106, 3.60 x 106 and 3.50 x 106 U-E/mL, respectively) and N. melanoleuca, N. mossambica and N. annulifera (7.41 x 106, 3.07 x 106 and 2.60 x 106 U-E/mL, respectively), but lower titers against the D. angusticeps and D. polylepis (1.87 x 106 and 1.67 x 106 U-E/mL). All the groups, except anti-N. melanoleuca, showed significant differences from the anti-Crotalic antivenom (7.55 x 106 U-E/mL). The affinity index of all the groups was high, ranging from 31% to 45%. Cross-recognition assays showed the recognition of proteins with similar molecular weight in the venoms and may indicate the possibility of paraspecific neutralization. The three monospecific antivenoms were able to provide in vivo protection. CONCLUSION:Our results ... |
format |
Article in Journal/Newspaper |
author |
Felipe Raimondi Guidolin Celso Pereira Caricati José Roberto Marcelino Wilmar Dias da Silva |
author_facet |
Felipe Raimondi Guidolin Celso Pereira Caricati José Roberto Marcelino Wilmar Dias da Silva |
author_sort |
Felipe Raimondi Guidolin |
title |
Development of Equine IgG Antivenoms against Major Snake Groups in Mozambique. |
title_short |
Development of Equine IgG Antivenoms against Major Snake Groups in Mozambique. |
title_full |
Development of Equine IgG Antivenoms against Major Snake Groups in Mozambique. |
title_fullStr |
Development of Equine IgG Antivenoms against Major Snake Groups in Mozambique. |
title_full_unstemmed |
Development of Equine IgG Antivenoms against Major Snake Groups in Mozambique. |
title_sort |
development of equine igg antivenoms against major snake groups in mozambique. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2016 |
url |
https://doi.org/10.1371/journal.pntd.0004325 https://doaj.org/article/f6ee1c4cc7b14e1290f44740b47651df |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 10, Iss 1, p e0004325 (2016) |
op_relation |
http://europepmc.org/articles/PMC4701360?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0004325 https://doaj.org/article/f6ee1c4cc7b14e1290f44740b47651df |
op_doi |
https://doi.org/10.1371/journal.pntd.0004325 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
10 |
container_issue |
1 |
container_start_page |
e0004325 |
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1766346870758572032 |