Detection of hepatitis A antibodies by ELISA using saliva as clinical samples
The possibility of detecting acute infection and immunity using body fluids that are easier to collect than blood, mainly in children, would facilitate the investigation and follow-up of outbreaks of hepatitis A (HAV). Our study was carried out to evaluate the detection of anti-HAV IgM, IgA and tota...
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Universidade de São Paulo (USP)
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ftdoajarticles:oai:doaj.org/article:f6e6083efe614181aee6842e13a8bd7a 2024-09-09T19:26:15+00:00 Detection of hepatitis A antibodies by ELISA using saliva as clinical samples Isabel Takano OBA Angela Maria Miranda SPINA Cláudia Patara SARACENI Marcílio Figueiredo LEMOS Rita de Cássia Ferreira Andrade SENHORAS Regina Célia MOREIRA Celso Francisco Hernandes GRANATO 2000-08-01T00:00:00Z https://doaj.org/article/f6e6083efe614181aee6842e13a8bd7a EN eng Universidade de São Paulo (USP) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652000000400004&tlng=en https://doaj.org/toc/1678-9946 1678-9946 https://doaj.org/article/f6e6083efe614181aee6842e13a8bd7a Revista do Instituto de Medicina Tropical de São Paulo, Vol 42, Iss 4, Pp 197-200 (2000) Hepatitis A Saliva Antibodies Vaccine Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2000 ftdoajarticles 2024-08-05T17:49:30Z The possibility of detecting acute infection and immunity using body fluids that are easier to collect than blood, mainly in children, would facilitate the investigation and follow-up of outbreaks of hepatitis A (HAV). Our study was carried out to evaluate the detection of anti-HAV IgM, IgA and total antibodies in saliva using serum samples as reference. Forty three paired serum and saliva samples were analyzed. From this total, 24 samples were obtained from children and 1 from one adult during the course of acute hepatitis A; an additional 18 samples were obtained from health professionals from Adolfo Lutz Institute. The sensitivity to detect anti-HAV IgM was 100% (95%CI: 79.1 to 100.0%), employing saliva as clinical samples. In detecting anti-HAV IgA, the sensitivity was 80.8% (95%CI: 60.0 to 92.7%) and for the total antibodies was 82.1% (95%CI: 62.4 to 93.2%). The specificity was 100% for each. The rate of agreement was high comparing the results of serum and saliva samples for detecting HAV antibodies. We conclude that saliva is an acceptable alternative specimen for diagnosing acute hepatitis A infection, and for screening individuals to receive hepatitis A vaccine or immunoglobulin. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Hepatitis A Saliva Antibodies Vaccine Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
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Hepatitis A Saliva Antibodies Vaccine Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Isabel Takano OBA Angela Maria Miranda SPINA Cláudia Patara SARACENI Marcílio Figueiredo LEMOS Rita de Cássia Ferreira Andrade SENHORAS Regina Célia MOREIRA Celso Francisco Hernandes GRANATO Detection of hepatitis A antibodies by ELISA using saliva as clinical samples |
topic_facet |
Hepatitis A Saliva Antibodies Vaccine Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
The possibility of detecting acute infection and immunity using body fluids that are easier to collect than blood, mainly in children, would facilitate the investigation and follow-up of outbreaks of hepatitis A (HAV). Our study was carried out to evaluate the detection of anti-HAV IgM, IgA and total antibodies in saliva using serum samples as reference. Forty three paired serum and saliva samples were analyzed. From this total, 24 samples were obtained from children and 1 from one adult during the course of acute hepatitis A; an additional 18 samples were obtained from health professionals from Adolfo Lutz Institute. The sensitivity to detect anti-HAV IgM was 100% (95%CI: 79.1 to 100.0%), employing saliva as clinical samples. In detecting anti-HAV IgA, the sensitivity was 80.8% (95%CI: 60.0 to 92.7%) and for the total antibodies was 82.1% (95%CI: 62.4 to 93.2%). The specificity was 100% for each. The rate of agreement was high comparing the results of serum and saliva samples for detecting HAV antibodies. We conclude that saliva is an acceptable alternative specimen for diagnosing acute hepatitis A infection, and for screening individuals to receive hepatitis A vaccine or immunoglobulin. |
format |
Article in Journal/Newspaper |
author |
Isabel Takano OBA Angela Maria Miranda SPINA Cláudia Patara SARACENI Marcílio Figueiredo LEMOS Rita de Cássia Ferreira Andrade SENHORAS Regina Célia MOREIRA Celso Francisco Hernandes GRANATO |
author_facet |
Isabel Takano OBA Angela Maria Miranda SPINA Cláudia Patara SARACENI Marcílio Figueiredo LEMOS Rita de Cássia Ferreira Andrade SENHORAS Regina Célia MOREIRA Celso Francisco Hernandes GRANATO |
author_sort |
Isabel Takano OBA |
title |
Detection of hepatitis A antibodies by ELISA using saliva as clinical samples |
title_short |
Detection of hepatitis A antibodies by ELISA using saliva as clinical samples |
title_full |
Detection of hepatitis A antibodies by ELISA using saliva as clinical samples |
title_fullStr |
Detection of hepatitis A antibodies by ELISA using saliva as clinical samples |
title_full_unstemmed |
Detection of hepatitis A antibodies by ELISA using saliva as clinical samples |
title_sort |
detection of hepatitis a antibodies by elisa using saliva as clinical samples |
publisher |
Universidade de São Paulo (USP) |
publishDate |
2000 |
url |
https://doaj.org/article/f6e6083efe614181aee6842e13a8bd7a |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista do Instituto de Medicina Tropical de São Paulo, Vol 42, Iss 4, Pp 197-200 (2000) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652000000400004&tlng=en https://doaj.org/toc/1678-9946 1678-9946 https://doaj.org/article/f6e6083efe614181aee6842e13a8bd7a |
_version_ |
1809895915143561216 |