Enzyme-Assisted Extraction of Bioactive Material from Chondrus crispus and Codium fragile and Its Effect on Herpes simplex Virus (HSV-1)
Codium fragile and Chondrus crispus are, respectively, green and red seaweeds which are abundant along the North Atlantic coasts. We investigated the chemical composition and antiviral activity of enzymatic extracts of C. fragile (CF) and C. crispus (CC). On a dry weight basis, CF consisted of 11% p...
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ftdoajarticles:oai:doaj.org/article:f6aed94317ba477792a0d1a7aa6ed41a 2023-05-15T17:34:32+02:00 Enzyme-Assisted Extraction of Bioactive Material from Chondrus crispus and Codium fragile and Its Effect on Herpes simplex Virus (HSV-1) Garima Kulshreshtha Anne-Sophie Burlot Christel Marty Alan Critchley Jeff Hafting Gilles Bedoux Nathalie Bourgougnon Balakrishnan Prithiviraj 2015-01-01T00:00:00Z https://doi.org/10.3390/md13010558 https://doaj.org/article/f6aed94317ba477792a0d1a7aa6ed41a EN eng MDPI AG http://www.mdpi.com/1660-3397/13/1/558 https://doaj.org/toc/1660-3397 1660-3397 doi:10.3390/md13010558 https://doaj.org/article/f6aed94317ba477792a0d1a7aa6ed41a Marine Drugs, Vol 13, Iss 1, Pp 558-580 (2015) Codium fragile Chondrus crispus red seaweeds sulfates Herpes simplex virus (HSV-1) Biology (General) QH301-705.5 article 2015 ftdoajarticles https://doi.org/10.3390/md13010558 2022-12-31T00:14:13Z Codium fragile and Chondrus crispus are, respectively, green and red seaweeds which are abundant along the North Atlantic coasts. We investigated the chemical composition and antiviral activity of enzymatic extracts of C. fragile (CF) and C. crispus (CC). On a dry weight basis, CF consisted of 11% protein, 31% neutral sugars, 0.8% sulfate, 0.6% uronic acids, and 49% ash, while CC contained 27% protein, 28% neutral sugars, 17% sulfate, 1.8% uronic acids, and 25% ash. Enzyme-assisted hydrolysis improved the extraction efficiency of bioactive materials. Commercial proteases and carbohydrases significantly improved (p ≤ 0.001) biomass yield (40%–70% dry matter) as compared to aqueous extraction (20%–25% dry matter). Moreover, enzymatic hydrolysis enhanced the recovery of protein, neutral sugars, uronic acids, and sulfates. The enzymatic hydrolysates exhibited significant activity against Herpes simplex virus (HSV-1) with EC50 of 77.6–126.8 μg/mL for CC and 36.5–41.3 μg/mL for CF, at a multiplicity of infection (MOI) of 0.001 ID50/cells without cytotoxity (1–200 μg/mL). The extracts obtained from proteases (P1) and carbohydrases (C3) were also effective at higher virus MOI of 0.01 ID50/cells without cytotoxity. Taken together, these results indicate the potential application of enzymatic hydrolysates of C. fragile and C. crispus in functional food and antiviral drug discovery. Article in Journal/Newspaper North Atlantic Directory of Open Access Journals: DOAJ Articles Marine Drugs 13 1 558 580 |
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language |
English |
topic |
Codium fragile Chondrus crispus red seaweeds sulfates Herpes simplex virus (HSV-1) Biology (General) QH301-705.5 |
spellingShingle |
Codium fragile Chondrus crispus red seaweeds sulfates Herpes simplex virus (HSV-1) Biology (General) QH301-705.5 Garima Kulshreshtha Anne-Sophie Burlot Christel Marty Alan Critchley Jeff Hafting Gilles Bedoux Nathalie Bourgougnon Balakrishnan Prithiviraj Enzyme-Assisted Extraction of Bioactive Material from Chondrus crispus and Codium fragile and Its Effect on Herpes simplex Virus (HSV-1) |
topic_facet |
Codium fragile Chondrus crispus red seaweeds sulfates Herpes simplex virus (HSV-1) Biology (General) QH301-705.5 |
description |
Codium fragile and Chondrus crispus are, respectively, green and red seaweeds which are abundant along the North Atlantic coasts. We investigated the chemical composition and antiviral activity of enzymatic extracts of C. fragile (CF) and C. crispus (CC). On a dry weight basis, CF consisted of 11% protein, 31% neutral sugars, 0.8% sulfate, 0.6% uronic acids, and 49% ash, while CC contained 27% protein, 28% neutral sugars, 17% sulfate, 1.8% uronic acids, and 25% ash. Enzyme-assisted hydrolysis improved the extraction efficiency of bioactive materials. Commercial proteases and carbohydrases significantly improved (p ≤ 0.001) biomass yield (40%–70% dry matter) as compared to aqueous extraction (20%–25% dry matter). Moreover, enzymatic hydrolysis enhanced the recovery of protein, neutral sugars, uronic acids, and sulfates. The enzymatic hydrolysates exhibited significant activity against Herpes simplex virus (HSV-1) with EC50 of 77.6–126.8 μg/mL for CC and 36.5–41.3 μg/mL for CF, at a multiplicity of infection (MOI) of 0.001 ID50/cells without cytotoxity (1–200 μg/mL). The extracts obtained from proteases (P1) and carbohydrases (C3) were also effective at higher virus MOI of 0.01 ID50/cells without cytotoxity. Taken together, these results indicate the potential application of enzymatic hydrolysates of C. fragile and C. crispus in functional food and antiviral drug discovery. |
format |
Article in Journal/Newspaper |
author |
Garima Kulshreshtha Anne-Sophie Burlot Christel Marty Alan Critchley Jeff Hafting Gilles Bedoux Nathalie Bourgougnon Balakrishnan Prithiviraj |
author_facet |
Garima Kulshreshtha Anne-Sophie Burlot Christel Marty Alan Critchley Jeff Hafting Gilles Bedoux Nathalie Bourgougnon Balakrishnan Prithiviraj |
author_sort |
Garima Kulshreshtha |
title |
Enzyme-Assisted Extraction of Bioactive Material from Chondrus crispus and Codium fragile and Its Effect on Herpes simplex Virus (HSV-1) |
title_short |
Enzyme-Assisted Extraction of Bioactive Material from Chondrus crispus and Codium fragile and Its Effect on Herpes simplex Virus (HSV-1) |
title_full |
Enzyme-Assisted Extraction of Bioactive Material from Chondrus crispus and Codium fragile and Its Effect on Herpes simplex Virus (HSV-1) |
title_fullStr |
Enzyme-Assisted Extraction of Bioactive Material from Chondrus crispus and Codium fragile and Its Effect on Herpes simplex Virus (HSV-1) |
title_full_unstemmed |
Enzyme-Assisted Extraction of Bioactive Material from Chondrus crispus and Codium fragile and Its Effect on Herpes simplex Virus (HSV-1) |
title_sort |
enzyme-assisted extraction of bioactive material from chondrus crispus and codium fragile and its effect on herpes simplex virus (hsv-1) |
publisher |
MDPI AG |
publishDate |
2015 |
url |
https://doi.org/10.3390/md13010558 https://doaj.org/article/f6aed94317ba477792a0d1a7aa6ed41a |
genre |
North Atlantic |
genre_facet |
North Atlantic |
op_source |
Marine Drugs, Vol 13, Iss 1, Pp 558-580 (2015) |
op_relation |
http://www.mdpi.com/1660-3397/13/1/558 https://doaj.org/toc/1660-3397 1660-3397 doi:10.3390/md13010558 https://doaj.org/article/f6aed94317ba477792a0d1a7aa6ed41a |
op_doi |
https://doi.org/10.3390/md13010558 |
container_title |
Marine Drugs |
container_volume |
13 |
container_issue |
1 |
container_start_page |
558 |
op_container_end_page |
580 |
_version_ |
1766133414371524608 |