Genome-Resolved Metagenomic Analysis Reveals Roles for Candidate Phyla and Other Microbial Community Members in Biogeochemical Transformations in Oil Reservoirs
ABSTRACT Oil reservoirs are major sites of methane production and carbon turnover, processes with significant impacts on energy resources and global biogeochemical cycles. We applied a cultivation-independent genomic approach to define microbial community membership and predict roles for specific or...
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ftdoajarticles:oai:doaj.org/article:f6a7f457a0044edd872aa46355d3bf1c 2023-05-15T13:09:10+02:00 Genome-Resolved Metagenomic Analysis Reveals Roles for Candidate Phyla and Other Microbial Community Members in Biogeochemical Transformations in Oil Reservoirs Ping Hu Lauren Tom Andrea Singh Brian C. Thomas Brett J. Baker Yvette M. Piceno Gary L. Andersen Jillian F. Banfield 2016-03-01T00:00:00Z https://doi.org/10.1128/mBio.01669-15 https://doaj.org/article/f6a7f457a0044edd872aa46355d3bf1c EN eng American Society for Microbiology https://journals.asm.org/doi/10.1128/mBio.01669-15 https://doaj.org/toc/2150-7511 doi:10.1128/mBio.01669-15 2150-7511 https://doaj.org/article/f6a7f457a0044edd872aa46355d3bf1c mBio, Vol 7, Iss 1 (2016) Microbiology QR1-502 article 2016 ftdoajarticles https://doi.org/10.1128/mBio.01669-15 2022-12-31T09:19:32Z ABSTRACT Oil reservoirs are major sites of methane production and carbon turnover, processes with significant impacts on energy resources and global biogeochemical cycles. We applied a cultivation-independent genomic approach to define microbial community membership and predict roles for specific organisms in biogeochemical transformations in Alaska North Slope oil fields. Produced water samples were collected from six locations between 1,128 m (24 to 27°C) and 2,743 m (80 to 83°C) below the surface. Microbial community complexity decreased with increasing temperature, and the potential to degrade hydrocarbon compounds was most prevalent in the lower-temperature reservoirs. Sulfate availability, rather than sulfate reduction potential, seems to be the limiting factor for sulfide production in some of the reservoirs under investigation. Most microorganisms in the intermediate- and higher-temperature samples were related to previously studied methanogenic and nonmethanogenic archaea and thermophilic bacteria, but one candidate phylum bacterium, a member of the Acetothermia (OP1), was present in Kuparuk sample K3. The greatest numbers of candidate phyla were recovered from the mesothermic reservoir samples SB1 and SB2. We reconstructed a nearly complete genome for an organism from the candidate phylum Parcubacteria (OD1) that was abundant in sample SB1. Consistent with prior findings for members of this lineage, the OD1 genome is small, and metabolic predictions support an obligately anaerobic, fermentation-based lifestyle. At moderate abundance in samples SB1 and SB2 were members of bacteria from other candidate phyla, including Microgenomates (OP11), Atribacteria (OP9), candidate phyla TA06 and WS6, and Marinimicrobia (SAR406). The results presented here elucidate potential roles of organisms in oil reservoir biological processes. IMPORTANCE The activities of microorganisms in oil reservoirs impact petroleum resource quality and the global carbon cycle. We show that bacteria belonging to candidate phyla are ... Article in Journal/Newspaper Alaska North Slope north slope Alaska Directory of Open Access Journals: DOAJ Articles mBio 7 1 |
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Microbiology QR1-502 |
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Microbiology QR1-502 Ping Hu Lauren Tom Andrea Singh Brian C. Thomas Brett J. Baker Yvette M. Piceno Gary L. Andersen Jillian F. Banfield Genome-Resolved Metagenomic Analysis Reveals Roles for Candidate Phyla and Other Microbial Community Members in Biogeochemical Transformations in Oil Reservoirs |
topic_facet |
Microbiology QR1-502 |
description |
ABSTRACT Oil reservoirs are major sites of methane production and carbon turnover, processes with significant impacts on energy resources and global biogeochemical cycles. We applied a cultivation-independent genomic approach to define microbial community membership and predict roles for specific organisms in biogeochemical transformations in Alaska North Slope oil fields. Produced water samples were collected from six locations between 1,128 m (24 to 27°C) and 2,743 m (80 to 83°C) below the surface. Microbial community complexity decreased with increasing temperature, and the potential to degrade hydrocarbon compounds was most prevalent in the lower-temperature reservoirs. Sulfate availability, rather than sulfate reduction potential, seems to be the limiting factor for sulfide production in some of the reservoirs under investigation. Most microorganisms in the intermediate- and higher-temperature samples were related to previously studied methanogenic and nonmethanogenic archaea and thermophilic bacteria, but one candidate phylum bacterium, a member of the Acetothermia (OP1), was present in Kuparuk sample K3. The greatest numbers of candidate phyla were recovered from the mesothermic reservoir samples SB1 and SB2. We reconstructed a nearly complete genome for an organism from the candidate phylum Parcubacteria (OD1) that was abundant in sample SB1. Consistent with prior findings for members of this lineage, the OD1 genome is small, and metabolic predictions support an obligately anaerobic, fermentation-based lifestyle. At moderate abundance in samples SB1 and SB2 were members of bacteria from other candidate phyla, including Microgenomates (OP11), Atribacteria (OP9), candidate phyla TA06 and WS6, and Marinimicrobia (SAR406). The results presented here elucidate potential roles of organisms in oil reservoir biological processes. IMPORTANCE The activities of microorganisms in oil reservoirs impact petroleum resource quality and the global carbon cycle. We show that bacteria belonging to candidate phyla are ... |
format |
Article in Journal/Newspaper |
author |
Ping Hu Lauren Tom Andrea Singh Brian C. Thomas Brett J. Baker Yvette M. Piceno Gary L. Andersen Jillian F. Banfield |
author_facet |
Ping Hu Lauren Tom Andrea Singh Brian C. Thomas Brett J. Baker Yvette M. Piceno Gary L. Andersen Jillian F. Banfield |
author_sort |
Ping Hu |
title |
Genome-Resolved Metagenomic Analysis Reveals Roles for Candidate Phyla and Other Microbial Community Members in Biogeochemical Transformations in Oil Reservoirs |
title_short |
Genome-Resolved Metagenomic Analysis Reveals Roles for Candidate Phyla and Other Microbial Community Members in Biogeochemical Transformations in Oil Reservoirs |
title_full |
Genome-Resolved Metagenomic Analysis Reveals Roles for Candidate Phyla and Other Microbial Community Members in Biogeochemical Transformations in Oil Reservoirs |
title_fullStr |
Genome-Resolved Metagenomic Analysis Reveals Roles for Candidate Phyla and Other Microbial Community Members in Biogeochemical Transformations in Oil Reservoirs |
title_full_unstemmed |
Genome-Resolved Metagenomic Analysis Reveals Roles for Candidate Phyla and Other Microbial Community Members in Biogeochemical Transformations in Oil Reservoirs |
title_sort |
genome-resolved metagenomic analysis reveals roles for candidate phyla and other microbial community members in biogeochemical transformations in oil reservoirs |
publisher |
American Society for Microbiology |
publishDate |
2016 |
url |
https://doi.org/10.1128/mBio.01669-15 https://doaj.org/article/f6a7f457a0044edd872aa46355d3bf1c |
genre |
Alaska North Slope north slope Alaska |
genre_facet |
Alaska North Slope north slope Alaska |
op_source |
mBio, Vol 7, Iss 1 (2016) |
op_relation |
https://journals.asm.org/doi/10.1128/mBio.01669-15 https://doaj.org/toc/2150-7511 doi:10.1128/mBio.01669-15 2150-7511 https://doaj.org/article/f6a7f457a0044edd872aa46355d3bf1c |
op_doi |
https://doi.org/10.1128/mBio.01669-15 |
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mBio |
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7 |
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1 |
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1766165204160217088 |