Trypanosoma cruzi Epimastigogenesis: A Method for Studying Trypomastigote to Epimastigote Transformation In Vivo
Trypanosoma cruzi epimastigogenesis naturally occurs in the intestine of the invertebrate host. It is not known whether the morphological changes that occur during transformation of bloodstream trypomastigotes to epimastigotes are identical for different T. cruzi isolates. This research shows a usef...
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Universidad del Zulia,Facultad de Medicina,Departamento de Enfermedades Infecciosas y Tropicales
2012
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ftdoajarticles:oai:doaj.org/article:f698c1e02b034be7a390d9e6f657cac6 2023-10-01T03:54:09+02:00 Trypanosoma cruzi Epimastigogenesis: A Method for Studying Trypomastigote to Epimastigote Transformation In Vivo María Navarro Angi Córdova Karla Fernández Rosa Arteaga Diana Graterol María Domínguez Ana De Lima Wilmer Pineda Víctor Contreras 2012-07-01T00:00:00Z https://doaj.org/article/f698c1e02b034be7a390d9e6f657cac6 EN ES eng spa Universidad del Zulia,Facultad de Medicina,Departamento de Enfermedades Infecciosas y Tropicales https://produccioncientificaluz.org/index.php/kasmera/article/view/4937 https://doaj.org/toc/0075-5222 https://doaj.org/toc/2477-9628 0075-5222 2477-9628 https://doaj.org/article/f698c1e02b034be7a390d9e6f657cac6 Kasmera, Vol 40, Iss 2, Pp 122-133 (2012) Trypanosoma cruzi epimastigogénesis in vitro e in vivo Rhodnius prolixus Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2012 ftdoajarticles 2023-09-03T00:47:38Z Trypanosoma cruzi epimastigogenesis naturally occurs in the intestine of the invertebrate host. It is not known whether the morphological changes that occur during transformation of bloodstream trypomastigotes to epimastigotes are identical for different T. cruzi isolates. This research shows a useful method for studying the events that occur during epimastigogenesis, comparing parasites from epidemiological sources. Rhodnius prolixus V stage nymphs were fed artificially with an ad hoc solution containing blood-like trypomastigotes and the morphological changes were examined during eight days. Anterior intestinal contents were removed by decapitation and squeezing the abdomen of the nymphs, which permitted obtaining quickly almost 100% of the inoculate with loads between 1.9 and 8.9 × 106 trypomastigotes/nymph. The number of parasites per nymph and morphological changes were determined using phase microscopy with Giemsa staining. The nymphs ingested 7.4 times their weight of the infecting solution with volumes between 101 e 357 μL (229 ± 66 mg); 50% of the ingest was eliminated as urine during the first 24 hours. The trypomastigotes transformed to rounded forms before evolving into epimastigotes following different kinetics according to the isolate. This method is proposed for rapid, quantitative study of the early events of epimastigogenesis for T. cruzi in vivo. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic |
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Directory of Open Access Journals: DOAJ Articles |
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topic |
Trypanosoma cruzi epimastigogénesis in vitro e in vivo Rhodnius prolixus Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Trypanosoma cruzi epimastigogénesis in vitro e in vivo Rhodnius prolixus Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 María Navarro Angi Córdova Karla Fernández Rosa Arteaga Diana Graterol María Domínguez Ana De Lima Wilmer Pineda Víctor Contreras Trypanosoma cruzi Epimastigogenesis: A Method for Studying Trypomastigote to Epimastigote Transformation In Vivo |
topic_facet |
Trypanosoma cruzi epimastigogénesis in vitro e in vivo Rhodnius prolixus Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Trypanosoma cruzi epimastigogenesis naturally occurs in the intestine of the invertebrate host. It is not known whether the morphological changes that occur during transformation of bloodstream trypomastigotes to epimastigotes are identical for different T. cruzi isolates. This research shows a useful method for studying the events that occur during epimastigogenesis, comparing parasites from epidemiological sources. Rhodnius prolixus V stage nymphs were fed artificially with an ad hoc solution containing blood-like trypomastigotes and the morphological changes were examined during eight days. Anterior intestinal contents were removed by decapitation and squeezing the abdomen of the nymphs, which permitted obtaining quickly almost 100% of the inoculate with loads between 1.9 and 8.9 × 106 trypomastigotes/nymph. The number of parasites per nymph and morphological changes were determined using phase microscopy with Giemsa staining. The nymphs ingested 7.4 times their weight of the infecting solution with volumes between 101 e 357 μL (229 ± 66 mg); 50% of the ingest was eliminated as urine during the first 24 hours. The trypomastigotes transformed to rounded forms before evolving into epimastigotes following different kinetics according to the isolate. This method is proposed for rapid, quantitative study of the early events of epimastigogenesis for T. cruzi in vivo. |
format |
Article in Journal/Newspaper |
author |
María Navarro Angi Córdova Karla Fernández Rosa Arteaga Diana Graterol María Domínguez Ana De Lima Wilmer Pineda Víctor Contreras |
author_facet |
María Navarro Angi Córdova Karla Fernández Rosa Arteaga Diana Graterol María Domínguez Ana De Lima Wilmer Pineda Víctor Contreras |
author_sort |
María Navarro |
title |
Trypanosoma cruzi Epimastigogenesis: A Method for Studying Trypomastigote to Epimastigote Transformation In Vivo |
title_short |
Trypanosoma cruzi Epimastigogenesis: A Method for Studying Trypomastigote to Epimastigote Transformation In Vivo |
title_full |
Trypanosoma cruzi Epimastigogenesis: A Method for Studying Trypomastigote to Epimastigote Transformation In Vivo |
title_fullStr |
Trypanosoma cruzi Epimastigogenesis: A Method for Studying Trypomastigote to Epimastigote Transformation In Vivo |
title_full_unstemmed |
Trypanosoma cruzi Epimastigogenesis: A Method for Studying Trypomastigote to Epimastigote Transformation In Vivo |
title_sort |
trypanosoma cruzi epimastigogenesis: a method for studying trypomastigote to epimastigote transformation in vivo |
publisher |
Universidad del Zulia,Facultad de Medicina,Departamento de Enfermedades Infecciosas y Tropicales |
publishDate |
2012 |
url |
https://doaj.org/article/f698c1e02b034be7a390d9e6f657cac6 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Kasmera, Vol 40, Iss 2, Pp 122-133 (2012) |
op_relation |
https://produccioncientificaluz.org/index.php/kasmera/article/view/4937 https://doaj.org/toc/0075-5222 https://doaj.org/toc/2477-9628 0075-5222 2477-9628 https://doaj.org/article/f698c1e02b034be7a390d9e6f657cac6 |
_version_ |
1778521522811437056 |