Analysis of spleen histopathology, splenocyte composition and haematological parameters in four strains of mice infected with Plasmodium berghei K173
Abstract Background Malaria is a fatal disease that presents clinically as a continuum of symptoms and severity, which are determined by complex host-parasite interactions. Clearance of infection is believed to be accomplished by the spleen and mononuclear phagocytic system (MPS), independent of art...
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ftdoajarticles:oai:doaj.org/article:f6399290321540f29a9b227d649037a0 2023-05-15T15:18:02+02:00 Analysis of spleen histopathology, splenocyte composition and haematological parameters in four strains of mice infected with Plasmodium berghei K173 Huajing Wang Shuo Li Zhao Cui Tingting Qin Hang Shi Ji Ma Lanfang Li Guihua Yu Tingliang Jiang Canghai Li 2021-06-01T00:00:00Z https://doi.org/10.1186/s12936-021-03786-z https://doaj.org/article/f6399290321540f29a9b227d649037a0 EN eng BMC https://doi.org/10.1186/s12936-021-03786-z https://doaj.org/toc/1475-2875 doi:10.1186/s12936-021-03786-z 1475-2875 https://doaj.org/article/f6399290321540f29a9b227d649037a0 Malaria Journal, Vol 20, Iss 1, Pp 1-12 (2021) Malaria Spleen Filtration Splenocyte Plasmodium berghei K173 Mouse Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2021 ftdoajarticles https://doi.org/10.1186/s12936-021-03786-z 2022-12-31T11:36:17Z Abstract Background Malaria is a fatal disease that presents clinically as a continuum of symptoms and severity, which are determined by complex host-parasite interactions. Clearance of infection is believed to be accomplished by the spleen and mononuclear phagocytic system (MPS), independent of artemisinin treatment. The spleen filters infected red blood cells (RBCs) from circulation through immune-mediated recognition of the infected RBCs followed by phagocytosis. This study evaluated the tolerance of four different strains of mice to Plasmodium berghei strain K173 (P. berghei K173), and the differences in the role of the spleen in controlling P. berghei K173 infection. Methods Using different strains of mice (C57BL/6, BALB/C, ICR, and KM mice) infected with P. berghei K173, the mechanisms leading to splenomegaly, histopathology, splenocyte activation and proliferation, and their relationship to the control of parasitaemia and host mortality were examined and evaluated. Results Survival time of mice infected with P. berghei K173 varied, although the infection was uniformly lethal. Mice of the C57BL/6 strain were the most resistant, while mice of the strain ICR were the most susceptible. BALB/c and KM mice were intermediate. In the course of P. berghei K173 infection, all infected mice experienced significant splenomegaly. Parasites were observed in the red pulp at 3 days post infection (dpi) in all animals. All spleens retained late trophozoite stages as well as a fraction of earlier ring-stage parasites. The percentages of macrophages in infected C57BL/6 and KM mice were higher than uninfected mice on 8 dpi. Spleens of infected ICR and KM mice exhibited structural disorganization and remodelling. Furthermore, parasitaemia was significantly higher in KM versus C57BL/6 mice at 8 dpi. The percentages of macrophages in ICR infected mice were lower than uninfected mice, and the parasitaemia was higher than other strains. Conclusions The results presented here demonstrate the rate of splenic mechanical filtration ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 20 1 |
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Directory of Open Access Journals: DOAJ Articles |
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English |
topic |
Malaria Spleen Filtration Splenocyte Plasmodium berghei K173 Mouse Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
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Malaria Spleen Filtration Splenocyte Plasmodium berghei K173 Mouse Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Huajing Wang Shuo Li Zhao Cui Tingting Qin Hang Shi Ji Ma Lanfang Li Guihua Yu Tingliang Jiang Canghai Li Analysis of spleen histopathology, splenocyte composition and haematological parameters in four strains of mice infected with Plasmodium berghei K173 |
topic_facet |
Malaria Spleen Filtration Splenocyte Plasmodium berghei K173 Mouse Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Malaria is a fatal disease that presents clinically as a continuum of symptoms and severity, which are determined by complex host-parasite interactions. Clearance of infection is believed to be accomplished by the spleen and mononuclear phagocytic system (MPS), independent of artemisinin treatment. The spleen filters infected red blood cells (RBCs) from circulation through immune-mediated recognition of the infected RBCs followed by phagocytosis. This study evaluated the tolerance of four different strains of mice to Plasmodium berghei strain K173 (P. berghei K173), and the differences in the role of the spleen in controlling P. berghei K173 infection. Methods Using different strains of mice (C57BL/6, BALB/C, ICR, and KM mice) infected with P. berghei K173, the mechanisms leading to splenomegaly, histopathology, splenocyte activation and proliferation, and their relationship to the control of parasitaemia and host mortality were examined and evaluated. Results Survival time of mice infected with P. berghei K173 varied, although the infection was uniformly lethal. Mice of the C57BL/6 strain were the most resistant, while mice of the strain ICR were the most susceptible. BALB/c and KM mice were intermediate. In the course of P. berghei K173 infection, all infected mice experienced significant splenomegaly. Parasites were observed in the red pulp at 3 days post infection (dpi) in all animals. All spleens retained late trophozoite stages as well as a fraction of earlier ring-stage parasites. The percentages of macrophages in infected C57BL/6 and KM mice were higher than uninfected mice on 8 dpi. Spleens of infected ICR and KM mice exhibited structural disorganization and remodelling. Furthermore, parasitaemia was significantly higher in KM versus C57BL/6 mice at 8 dpi. The percentages of macrophages in ICR infected mice were lower than uninfected mice, and the parasitaemia was higher than other strains. Conclusions The results presented here demonstrate the rate of splenic mechanical filtration ... |
format |
Article in Journal/Newspaper |
author |
Huajing Wang Shuo Li Zhao Cui Tingting Qin Hang Shi Ji Ma Lanfang Li Guihua Yu Tingliang Jiang Canghai Li |
author_facet |
Huajing Wang Shuo Li Zhao Cui Tingting Qin Hang Shi Ji Ma Lanfang Li Guihua Yu Tingliang Jiang Canghai Li |
author_sort |
Huajing Wang |
title |
Analysis of spleen histopathology, splenocyte composition and haematological parameters in four strains of mice infected with Plasmodium berghei K173 |
title_short |
Analysis of spleen histopathology, splenocyte composition and haematological parameters in four strains of mice infected with Plasmodium berghei K173 |
title_full |
Analysis of spleen histopathology, splenocyte composition and haematological parameters in four strains of mice infected with Plasmodium berghei K173 |
title_fullStr |
Analysis of spleen histopathology, splenocyte composition and haematological parameters in four strains of mice infected with Plasmodium berghei K173 |
title_full_unstemmed |
Analysis of spleen histopathology, splenocyte composition and haematological parameters in four strains of mice infected with Plasmodium berghei K173 |
title_sort |
analysis of spleen histopathology, splenocyte composition and haematological parameters in four strains of mice infected with plasmodium berghei k173 |
publisher |
BMC |
publishDate |
2021 |
url |
https://doi.org/10.1186/s12936-021-03786-z https://doaj.org/article/f6399290321540f29a9b227d649037a0 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 20, Iss 1, Pp 1-12 (2021) |
op_relation |
https://doi.org/10.1186/s12936-021-03786-z https://doaj.org/toc/1475-2875 doi:10.1186/s12936-021-03786-z 1475-2875 https://doaj.org/article/f6399290321540f29a9b227d649037a0 |
op_doi |
https://doi.org/10.1186/s12936-021-03786-z |
container_title |
Malaria Journal |
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20 |
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1 |
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1766348268652986368 |