Estimating the genetic structure of Triatoma dimidiata (Hemiptera: Reduviidae) and the transmission dynamics of Trypanosoma cruzi in Boyacá, eastern Colombia.
Chagas disease is considered a public health issue in Colombia, where many regions are endemic. Triatoma dimidiata is an important vector after Rhodnius prolixus, and it is gaining importance in Boyacá, eastern Colombia. Following the recent elimination of R. prolixus in the region, it is pivotal to...
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ftdoajarticles:oai:doaj.org/article:f60932fd4a57418e95dd7f1ea9309536 2023-05-15T15:13:12+02:00 Estimating the genetic structure of Triatoma dimidiata (Hemiptera: Reduviidae) and the transmission dynamics of Trypanosoma cruzi in Boyacá, eastern Colombia. Natalia Velásquez-Ortiz Carolina Hernández Omar Cantillo-Barraza Manuel Medina Mabel Medina-Alfonso Sandra Suescún-Carrero Marina Muñoz Laura Vega Sergio Castañeda Lissa Cruz-Saavedra Nathalia Ballesteros Juan David Ramírez 2022-07-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0010534 https://doaj.org/article/f60932fd4a57418e95dd7f1ea9309536 EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0010534 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0010534 https://doaj.org/article/f60932fd4a57418e95dd7f1ea9309536 PLoS Neglected Tropical Diseases, Vol 16, Iss 7, p e0010534 (2022) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2022 ftdoajarticles https://doi.org/10.1371/journal.pntd.0010534 2022-12-30T23:10:05Z Chagas disease is considered a public health issue in Colombia, where many regions are endemic. Triatoma dimidiata is an important vector after Rhodnius prolixus, and it is gaining importance in Boyacá, eastern Colombia. Following the recent elimination of R. prolixus in the region, it is pivotal to understand the behavior of T. dimidiata and the transmission dynamics of T. cruzi. We used qPCR and Next Generation Sequencing (NGS) to evaluate T. cruzi infection, parasite load, feeding profiles, and T. cruzi genotyping for T. dimidiata specimens collected in nine municipalities in Boyacá and explored T. dimidiata population genetics. We found that T. dimidiata populations are composed by a single population with similar genetic characteristics that present infection rates up to 70%, high parasite loads up to 1.46 × 109 parasite-equivalents/mL, a feeding behavior that comprises at least 17 domestic, synanthropic and sylvatic species, and a wide diversity of TcI genotypes even within a single specimen. These results imply that T. dimidiata behavior is similar to other successful vectors, having a wide variety of blood sources and contributing to the circulation of different genotypes of the parasite, highlighting its importance for T. cruzi transmission and risk for humans. In the light of the elimination of R. prolixus in Boyacá and the results we found, we suggest that T. dimidiata should become a new target for vector control programs. We hope this study provides enough information to enhance surveillance programs and a future effective interruption of T. cruzi vector transmission in endemic regions. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 16 7 e0010534 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
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English |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Natalia Velásquez-Ortiz Carolina Hernández Omar Cantillo-Barraza Manuel Medina Mabel Medina-Alfonso Sandra Suescún-Carrero Marina Muñoz Laura Vega Sergio Castañeda Lissa Cruz-Saavedra Nathalia Ballesteros Juan David Ramírez Estimating the genetic structure of Triatoma dimidiata (Hemiptera: Reduviidae) and the transmission dynamics of Trypanosoma cruzi in Boyacá, eastern Colombia. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Chagas disease is considered a public health issue in Colombia, where many regions are endemic. Triatoma dimidiata is an important vector after Rhodnius prolixus, and it is gaining importance in Boyacá, eastern Colombia. Following the recent elimination of R. prolixus in the region, it is pivotal to understand the behavior of T. dimidiata and the transmission dynamics of T. cruzi. We used qPCR and Next Generation Sequencing (NGS) to evaluate T. cruzi infection, parasite load, feeding profiles, and T. cruzi genotyping for T. dimidiata specimens collected in nine municipalities in Boyacá and explored T. dimidiata population genetics. We found that T. dimidiata populations are composed by a single population with similar genetic characteristics that present infection rates up to 70%, high parasite loads up to 1.46 × 109 parasite-equivalents/mL, a feeding behavior that comprises at least 17 domestic, synanthropic and sylvatic species, and a wide diversity of TcI genotypes even within a single specimen. These results imply that T. dimidiata behavior is similar to other successful vectors, having a wide variety of blood sources and contributing to the circulation of different genotypes of the parasite, highlighting its importance for T. cruzi transmission and risk for humans. In the light of the elimination of R. prolixus in Boyacá and the results we found, we suggest that T. dimidiata should become a new target for vector control programs. We hope this study provides enough information to enhance surveillance programs and a future effective interruption of T. cruzi vector transmission in endemic regions. |
format |
Article in Journal/Newspaper |
author |
Natalia Velásquez-Ortiz Carolina Hernández Omar Cantillo-Barraza Manuel Medina Mabel Medina-Alfonso Sandra Suescún-Carrero Marina Muñoz Laura Vega Sergio Castañeda Lissa Cruz-Saavedra Nathalia Ballesteros Juan David Ramírez |
author_facet |
Natalia Velásquez-Ortiz Carolina Hernández Omar Cantillo-Barraza Manuel Medina Mabel Medina-Alfonso Sandra Suescún-Carrero Marina Muñoz Laura Vega Sergio Castañeda Lissa Cruz-Saavedra Nathalia Ballesteros Juan David Ramírez |
author_sort |
Natalia Velásquez-Ortiz |
title |
Estimating the genetic structure of Triatoma dimidiata (Hemiptera: Reduviidae) and the transmission dynamics of Trypanosoma cruzi in Boyacá, eastern Colombia. |
title_short |
Estimating the genetic structure of Triatoma dimidiata (Hemiptera: Reduviidae) and the transmission dynamics of Trypanosoma cruzi in Boyacá, eastern Colombia. |
title_full |
Estimating the genetic structure of Triatoma dimidiata (Hemiptera: Reduviidae) and the transmission dynamics of Trypanosoma cruzi in Boyacá, eastern Colombia. |
title_fullStr |
Estimating the genetic structure of Triatoma dimidiata (Hemiptera: Reduviidae) and the transmission dynamics of Trypanosoma cruzi in Boyacá, eastern Colombia. |
title_full_unstemmed |
Estimating the genetic structure of Triatoma dimidiata (Hemiptera: Reduviidae) and the transmission dynamics of Trypanosoma cruzi in Boyacá, eastern Colombia. |
title_sort |
estimating the genetic structure of triatoma dimidiata (hemiptera: reduviidae) and the transmission dynamics of trypanosoma cruzi in boyacá, eastern colombia. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2022 |
url |
https://doi.org/10.1371/journal.pntd.0010534 https://doaj.org/article/f60932fd4a57418e95dd7f1ea9309536 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 16, Iss 7, p e0010534 (2022) |
op_relation |
https://doi.org/10.1371/journal.pntd.0010534 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0010534 https://doaj.org/article/f60932fd4a57418e95dd7f1ea9309536 |
op_doi |
https://doi.org/10.1371/journal.pntd.0010534 |
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PLOS Neglected Tropical Diseases |
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16 |
container_issue |
7 |
container_start_page |
e0010534 |
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1766343787042308096 |