Isoenzymatic genotyping of Staphylococcus aureus from dairy cattle and human clinical environments reveal evolutionary divergences
ABSTRACT Background The genetic variability of 610 S. aureus isolates from the hands of professional dentists (A), dental clinic environment air (B), bovine milk from cows with and without mastitis (C), an insufflator for milking equipment (D) and milking environment air (E) was studied by isoenzyme...
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ftdoajarticles:oai:doaj.org/article:f5ee5d4166204b3bbe041749fa288e11 2024-09-09T19:26:29+00:00 Isoenzymatic genotyping of Staphylococcus aureus from dairy cattle and human clinical environments reveal evolutionary divergences Marcelo Fabiano Gomes Boriollo Rodrigo Carlos Bassi José Francisco Höfling 2018-09-01T00:00:00Z https://doi.org/10.1590/s1678-9946201860054 https://doaj.org/article/f5ee5d4166204b3bbe041749fa288e11 EN eng Universidade de São Paulo (USP) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652018005000229&lng=en&tlng=en https://doaj.org/toc/1678-9946 1678-9946 doi:10.1590/s1678-9946201860054 https://doaj.org/article/f5ee5d4166204b3bbe041749fa288e11 Revista do Instituto de Medicina Tropical de São Paulo, Vol 60, Iss 0 (2018) Staphylococcus aureus Genetic diversity Environment and anatomical site Propagation dynamics MLEE Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2018 ftdoajarticles https://doi.org/10.1590/s1678-9946201860054 2024-08-05T17:49:30Z ABSTRACT Background The genetic variability of 610 S. aureus isolates from the hands of professional dentists (A), dental clinic environment air (B), bovine milk from cows with and without mastitis (C), an insufflator for milking equipment (D) and milking environment air (E) was studied by isoenzyme genotyping and genetic and cluster analysis. Results Monoclonal and polyclonal patterns of S. aureus were detected in every bacterial population; however, isolates belonging to the same strain were not found among the populations, suggesting the genetic heterogeneity and the intrapopulation spread of strains. Genetic relationship analysis revealed the co-existence of highly related strains at low frequency among populations. Conclusion The data suggest that some strains can adapt and colonize new epidemiologically unrelated habitats. Consequently, the occurrence of an epidemiological genotypic identity can assume a dynamic character (spread to new habitats), however infrequently. A tendency of microevolutionary and genetic divergences among populations of S. aureus from human sources (AB) and bovine milk (DE), and especially the mammary quarter (C), is also suggested. This research can contribute to the knowledge on the distribution and dissemination of strains and the implementation of control measures and eradication of S. aureus in important dental clinic environments, as well as animal environments and dairy production. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Revista do Instituto de Medicina Tropical de São Paulo 60 0 |
institution |
Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Staphylococcus aureus Genetic diversity Environment and anatomical site Propagation dynamics MLEE Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Staphylococcus aureus Genetic diversity Environment and anatomical site Propagation dynamics MLEE Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Marcelo Fabiano Gomes Boriollo Rodrigo Carlos Bassi José Francisco Höfling Isoenzymatic genotyping of Staphylococcus aureus from dairy cattle and human clinical environments reveal evolutionary divergences |
topic_facet |
Staphylococcus aureus Genetic diversity Environment and anatomical site Propagation dynamics MLEE Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
ABSTRACT Background The genetic variability of 610 S. aureus isolates from the hands of professional dentists (A), dental clinic environment air (B), bovine milk from cows with and without mastitis (C), an insufflator for milking equipment (D) and milking environment air (E) was studied by isoenzyme genotyping and genetic and cluster analysis. Results Monoclonal and polyclonal patterns of S. aureus were detected in every bacterial population; however, isolates belonging to the same strain were not found among the populations, suggesting the genetic heterogeneity and the intrapopulation spread of strains. Genetic relationship analysis revealed the co-existence of highly related strains at low frequency among populations. Conclusion The data suggest that some strains can adapt and colonize new epidemiologically unrelated habitats. Consequently, the occurrence of an epidemiological genotypic identity can assume a dynamic character (spread to new habitats), however infrequently. A tendency of microevolutionary and genetic divergences among populations of S. aureus from human sources (AB) and bovine milk (DE), and especially the mammary quarter (C), is also suggested. This research can contribute to the knowledge on the distribution and dissemination of strains and the implementation of control measures and eradication of S. aureus in important dental clinic environments, as well as animal environments and dairy production. |
format |
Article in Journal/Newspaper |
author |
Marcelo Fabiano Gomes Boriollo Rodrigo Carlos Bassi José Francisco Höfling |
author_facet |
Marcelo Fabiano Gomes Boriollo Rodrigo Carlos Bassi José Francisco Höfling |
author_sort |
Marcelo Fabiano Gomes Boriollo |
title |
Isoenzymatic genotyping of Staphylococcus aureus from dairy cattle and human clinical environments reveal evolutionary divergences |
title_short |
Isoenzymatic genotyping of Staphylococcus aureus from dairy cattle and human clinical environments reveal evolutionary divergences |
title_full |
Isoenzymatic genotyping of Staphylococcus aureus from dairy cattle and human clinical environments reveal evolutionary divergences |
title_fullStr |
Isoenzymatic genotyping of Staphylococcus aureus from dairy cattle and human clinical environments reveal evolutionary divergences |
title_full_unstemmed |
Isoenzymatic genotyping of Staphylococcus aureus from dairy cattle and human clinical environments reveal evolutionary divergences |
title_sort |
isoenzymatic genotyping of staphylococcus aureus from dairy cattle and human clinical environments reveal evolutionary divergences |
publisher |
Universidade de São Paulo (USP) |
publishDate |
2018 |
url |
https://doi.org/10.1590/s1678-9946201860054 https://doaj.org/article/f5ee5d4166204b3bbe041749fa288e11 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista do Instituto de Medicina Tropical de São Paulo, Vol 60, Iss 0 (2018) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652018005000229&lng=en&tlng=en https://doaj.org/toc/1678-9946 1678-9946 doi:10.1590/s1678-9946201860054 https://doaj.org/article/f5ee5d4166204b3bbe041749fa288e11 |
op_doi |
https://doi.org/10.1590/s1678-9946201860054 |
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Revista do Instituto de Medicina Tropical de São Paulo |
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60 |
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